公开(公告)号：US11814641B2

公开(公告)日：2023-11-14

申请号：US17192561

申请日：2021-03-04

申请人： AUTOLUS LIMITED

发明人： Martin Pulé ,  Leila Mekkaoui

IPC分类号： C12N15/86 ,  C12N15/867 ,  C12N5/0783 ,  C12N7/00 ,  C12N15/49 ,  C07K14/725 ,  C07K16/28 ,  A61K35/76 ,  A61K38/00 ,  C07K16/00 ,  C07K14/00 ,  A61K39/00

CPC分类号： C12N15/86 ,  A61K35/76 ,  A61K38/00 ,  C07K14/00 ,  C07K14/7051 ,  C07K16/00 ,  C07K16/2809 ,  C07K16/2818 ,  A61K2039/507 ,  C07K2317/622 ,  C07K2317/75 ,  C12N2740/13032 ,  C12N2740/13045 ,  C12N2740/13052 ,  C12N2740/15032 ,  C12N2740/15045 ,  C12N2740/15052 ,  C12N2810/852 ,  C12N2810/855 ,  C12N2810/859

摘要： The present invention provides a retroviral or lentiviral vector having a viral envelope which comprises: (i) a mitogenic T-cell activating transmembrane protein which comprises a mitogenic domain and a transmembrane domain; and/or (ii) a cytokine-based T-cell activating transmembrane protein which comprises a cytokine domain and a transmembrane domain, wherein the mitogenic or cytokine-based T-cell activating transmembrane protein is not part of a viral envelope glycoprotein. When cells such as T-cells of Natural Killer cells are transduced by such a viral vector, they are simultaneously activated by the mitogenic T-cell activating transmembrane protein and/or the cytokine-based T-cell activating transmembrane protein.

公开(公告)号：US11795474B2

公开(公告)日：2023-10-24

申请号：US16606281

申请日：2018-04-18

申请人： GlaxoSmithKline Intellectual Property Development Limited

发明人： Conrad Vink

IPC分类号： C12N15/00 ,  C12N15/86 ,  C07K14/16

CPC分类号： C12N15/86 ,  C07K14/161 ,  C07K14/162 ,  C12N2740/13052 ,  C12N2740/15052 ,  C12N2740/16052 ,  C12N2799/027 ,  C12N2830/003 ,  C12N2830/006 ,  C12N2830/60

摘要： The invention relates to retroviral producer cell comprising nucleic acid sequences encoding: gag and pol proteins; envelope protein or a functional substitute thereof; amplifiable selection marker; and the RNA genome of the retroviral vector particle, wherein said nucleic acid sequences are all integrated at a single locus within the retroviral producer cell genome. The invention also relates to nucleic acid vectors comprising a non-mammalian origin of replication and the ability to hold at least 25 kilobases (kb) of DNA, characterized in that said nucleic acid vector comprises retroviral nucleic acid sequences encoding: gag and pol proteins, and an env protein or a functional substitute thereof. The nucleic acid vector additionally comprises nucleic acid sequences encoding an amplifiable selection marker. The invention also relates to uses and methods using said nucleic acid vector in order to produce stable retroviral packaging and producer cell lines.

公开(公告)号：US09023648B2

公开(公告)日：2015-05-05

申请号：US12980938

申请日：2010-12-29

申请人： Mario Stevenson ,  Rajnish Kaushik ,  Xiaonan Zhu

发明人： Mario Stevenson ,  Rajnish Kaushik ,  Xiaonan Zhu

IPC分类号： C12N15/00 ,  C12N15/86 ,  A61K39/12 ,  A61K39/21 ,  C07K14/005 ,  C12N7/00 ,  A61K48/00

CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2740/13052 ,  C12N2740/15022 ,  C12N2740/15033 ,  C12N2740/16043 ,  C12N2740/16045 ,  C12N2740/16052

摘要： The invention is directed to a chimeric gammaretrovirus comprising an gammaretroviral virion which contains a lentiviral Vpx protein and methods of use thereof. In a particular aspect, the chimeric gammaretrovirus is a chimeric murine leukemia virus (MLV) comprising an MLV virion which contains a lentiviral Vpx protein. The invention is also directed to use of the chimeric gammaretrovirus to produce a MLV that can transduce a non-dividing cell (G1/S/G2), transduce a non-dividing cell; enhance the ability of a MLV to transduce a non-dividing cell; transduce a quiescent (G0) cell; and enhance the ability of a human immunodeficiency virus 1 (HIV-1) to transduce a quiescent (G0) cell.

摘要翻译： 本发明涉及包含含有慢病毒Vpx蛋白的瘤胃病毒病毒粒子的嵌合型马马虎病毒及其使用方法。 在特定方面，嵌合型马马克病毒是包含含有慢病毒Vpx蛋白的MLV病毒粒子的嵌合鼠白血病病毒（MLV）。 本发明还涉及使用嵌合型马马克病毒产生可以转导非分裂细胞（G1 / S / G2），转导非分裂细胞的MLV; 增强MLV转导不分裂细胞的能力; 转移静止（G0）细胞; 并增强人类免疫缺陷病毒1（HIV-1）转导静止（G0）细胞的能力。

公开(公告)号：US08703480B1

公开(公告)日：2014-04-22

申请号：US09007123

申请日：1998-01-14

申请人： Dakai Liu ,  Elazar Rabbani

发明人： Dakai Liu ,  Elazar Rabbani

IPC分类号： A61K39/295 ,  A61K39/145

CPC分类号： C12N15/86 ,  C12N2740/11043 ,  C12N2740/13043 ,  C12N2740/13044 ,  C12N2740/13052 ,  C12N2750/14143 ,  C12N2750/14151 ,  C12N2810/6054 ,  C12N2810/609

摘要： Provided are novel vectors comprising virus or viral portions in which biological function is effected by components on the surface or envelope. Biological function includes viral nucleic acid propagation and delivery in the same vector as well as other important functions such as enhanced target cell specificity and other favorable properties for endocytosis, nuclear localization, integration into the target cell genome and enhanced transduction efficiency functions. Also provided by this invention are chimeric cells useful as packaging cell lines or as target cells or even as both. By virtue of their unique sequences, such chimeric cells provide the machinery for viral nucleic acid propagation and packaging combined with other useful properties for delivering genetic information to any target cells of interest. Also provided are other vectors including those comprising initial nucleic acid wherein the initial nucleic acid has been modified and novel reconstituted vectors and fused vectors.

摘要翻译： 提供了包含病毒或病毒部分的新载体，其中生物功能由表面或信封上的组分实现。 生物学功能包括在相同载体中的病毒核酸繁殖和递送以及其他重要功能，例如增强的靶细胞特异性和其他有利的内吞，核定位，整合入靶细胞基因组和增强的转导效率功能的有利特征。 本发明还提供了可用作包装细胞系或靶细胞或甚至两者的嵌合细胞。 由于其独特的序列，这种嵌合细胞为病毒核酸传播和包装提供了与用于将遗传信息传递给感兴趣的任何靶细胞的其它有用特性的机制。 还提供了其它载体，包括那些包含初始核酸的载体，其中初始核酸已经被修饰和新的重构载体和融合载体。

公开(公告)号：US20120087894A1

公开(公告)日：2012-04-12

申请号：US13376827

申请日：2010-06-17

申请人： Douglas J. Jolly ,  Carlos Ibanez

发明人： Douglas J. Jolly ,  Carlos Ibanez

IPC分类号： C12N15/867 ,  A61K35/76 ,  A61P37/02 ,  A61P25/16 ,  A61P25/28 ,  C12N5/10 ,  A61P35/00

CPC分类号： C12N15/86 ,  C12N2740/13043 ,  C12N2740/13051 ,  C12N2740/13052

摘要： The disclosure provide cell lines and methods for the production of vectors and viral particles useful in gene therapy.

摘要翻译： 本公开提供用于生产用于基因治疗的载体和病毒颗粒的细胞系和方法。

公开(公告)号：US20060110361A1

公开(公告)日：2006-05-25

申请号：US10520745

申请日：2003-07-11

申请人： Colin Casimir

发明人： Colin Casimir

IPC分类号： A61K48/00 ,  C12N15/867

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N7/00 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2740/13051 ,  C12N2740/13052 ,  C12N2740/13061 ,  C12N2810/851 ,  C12N2810/859

摘要： The present invention relates to a method for packaging viral particles such that one or more peptides on the surface of the article are derived from the packaging cell. By incorporating certain peptides it is possible to target viral particles to specific cell types. Such a system is of use, for example, in gene therapy treatments.

摘要翻译： 本发明涉及包装病毒颗粒的方法，使得制品表面上的一种或多种肽源自包装细胞。 通过掺入某些肽，可能将病毒颗粒靶向特定的细胞类型。 这样的系统在例如基因疗法治疗中是有用的。

公开(公告)号：US20030199093A1

公开(公告)日：2003-10-23

申请号：US10260516

申请日：2002-10-01

发明人： Mitchell H. Finer ,  Margo R. Roberts ,  Thomas J. Dull ,  Krisztina M. Zsebo ,  Lu Qin ,  Deborah A. Farson ,  Keegan Cooke

IPC分类号： C12N015/867 ,  C12N007/00 ,  C12N005/06

CPC分类号： C12N7/00 ,  A61K48/00 ,  C07K14/005 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/721 ,  C07K16/10 ,  C07K2319/00 ,  C12N15/86 ,  C12N15/87 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/13062

摘要： The invention provides a novel retroviral packaging system, in which retroviral packaging plasmids and packagable vector transcripts are produced from high expression plasmids after stable or transient transfection in mammalian cells. High titers of recombinant retrovirus are produced in these transfected mammalian cells and can then transduce a mammalian target cell by cocultivation or supernatant infection. The methods of the invention include the use of the novel retroviral packaging plasmids and vectors to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation or supernatant infection at high efficiencies. The invention is useful for the rapid production of high titer viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.

公开(公告)号：US06489167B1

公开(公告)日：2002-12-03

申请号：US09147920

申请日：1999-07-12

申请人： Richard Morgan ,  Jarmo Wahlfors ,  Kleanthis Xanthopoulos

发明人： Richard Morgan ,  Jarmo Wahlfors ,  Kleanthis Xanthopoulos

IPC分类号： C12N1586

CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2740/13052

摘要： This invention provides a Togavirus-amplified retrovirus vector and a novel method for packaging a retrovirus cassette that contains a heterologous nucleic acid, which is amplified in a packaging cell cytoplasm by a Togavirus vector. The retroviral cassette is packaged into infectious retrovirus particles by retroviral packaging cells. These retroviral particles carrying the retroviral packaging cassette are then used to infect host cells.

摘要翻译： 本发明提供了一种披膜病毒扩增的逆转录病毒载体和用于包装逆转录病毒盒的新方法，其包含异源核酸，其通过番薯病毒载体在包装细胞质细胞中扩增。 逆转录病毒盒通过逆转录病毒包装细胞包装成感染性逆转录病毒颗粒。 携带逆转录病毒包装盒的这些逆转录病毒颗粒然后用于感染宿主细胞。

公开(公告)号：US20020173038A1

公开(公告)日：2002-11-21

申请号：US09147920

申请日：1999-07-12

发明人： RICHARD MORGAN ,  JARMO WAHLFORS ,  KLEANTHIS XANTHOPOULOS

IPC分类号： C12Q001/70 ,  C12P013/14 ,  C12N007/00 ,  C12N007/01 ,  C12N015/86

CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2740/13052

摘要： This invention provides a Togavirus-amplified retrovirus vector and a novel method for packaging a retrovirus cassette that contains a heterologous nucleic acid, which is amplified in a packaging cell cytoplasm by a Togavirus vector. The retroviral cassette is packaged into infectious retrovirus particles by retroviral packaging cells. These retroviral particles carrying the retroviral packaging cassette are then used to infect host cells.

摘要翻译： 本发明提供了一种披膜病毒扩增的逆转录病毒载体和用于包装逆转录病毒盒的新方法，其包含异源核酸，其通过番薯病毒载体在包装细胞质细胞中扩增。 逆转录病毒盒通过逆转录病毒包装细胞包装成感染性逆转录病毒颗粒。 携带逆转录病毒包装盒的这些逆转录病毒颗粒然后用于感染宿主细胞。

公开(公告)号：US06410316B1

公开(公告)日：2002-06-25

申请号：US09526983

申请日：2000-03-15

申请人： Philip L. Sheridan ,  Mordechai Bodner ,  Nicholas J. DePolo ,  Sybille Sauter ,  Stephen M. W. Chang

发明人： Philip L. Sheridan ,  Mordechai Bodner ,  Nicholas J. DePolo ,  Sybille Sauter ,  Stephen M. W. Chang

IPC分类号： C12N1500

CPC分类号： C12N7/00 ,  A61K48/00 ,  A61K48/0091 ,  C07K14/005 ,  C12N15/86 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2760/20134 ,  C12N2760/20222

摘要： Retroviral vector particle producing cells are provided, wherein the cell (a) has greater than 5 stably integrated copies of a retroviral vector construct; (b) produces greater than 10 infectious recombinant retroviral vector particles per cell per day; and (c) produces replication incompetent retroviral vector particles.

摘要翻译： 提供了逆转录病毒载体产生细胞，其中细胞（a）具有大于5个逆转录病毒载体构建体的稳定整合的拷贝; （b）每个细胞每天产生大于10个感染性重组逆转录病毒载体颗粒; 和（c）产生复制无能的逆转录病毒载体颗粒。