公开(公告)号：US20210310023A1

公开(公告)日：2021-10-07

申请号：US16905930

申请日：2020-06-19

申请人： Institute of Hydrobiology, Chinese Academy of Sciences

发明人： Zongbin Cui ,  Tong Zhou ,  Bolan Zhou ,  Yong Long ,  Guili Song ,  Qing Li ,  Zuoyan Zhu

IPC分类号： C12N15/85 ,  A01K67/027

摘要： A method for preparing a fish skin mucous gland bioreactor and its application, including: identifying genes specifically expressed in fish skin mucinous gland cells, promoters and secreted protein signal peptides, constructing transgenic expression vectors that can specifically express endogenous or heterologous biologically active substances in fish skin and mucous gland cells, developing stable genetic and transgenic fish that secrete bioactive substances into fish mucus, and using bioactive substances secreted by mucus glands for animal and plant growth, stress resistance and disease resistance, human health care and disease prevention, and commercial enzymes. The fish skin mucous gland bioreactor developed by the invention has the characteristics of easy breeding and expansion, more skin mucus secretion, convenient mucus collection, and easy purification of bioactive substances, and can realize the large-scale production of fish skin mucous gland bioreactor and efficient application.