公开(公告)号：US20210310023A1

公开(公告)日：2021-10-07

申请号：US16905930

申请日：2020-06-19

申请人： Institute of Hydrobiology, Chinese Academy of Sciences

发明人： Zongbin Cui ,  Tong Zhou ,  Bolan Zhou ,  Yong Long ,  Guili Song ,  Qing Li ,  Zuoyan Zhu

IPC分类号： C12N15/85 ,  A01K67/027

摘要： A method for preparing a fish skin mucous gland bioreactor and its application, including: identifying genes specifically expressed in fish skin mucinous gland cells, promoters and secreted protein signal peptides, constructing transgenic expression vectors that can specifically express endogenous or heterologous biologically active substances in fish skin and mucous gland cells, developing stable genetic and transgenic fish that secrete bioactive substances into fish mucus, and using bioactive substances secreted by mucus glands for animal and plant growth, stress resistance and disease resistance, human health care and disease prevention, and commercial enzymes. The fish skin mucous gland bioreactor developed by the invention has the characteristics of easy breeding and expansion, more skin mucus secretion, convenient mucus collection, and easy purification of bioactive substances, and can realize the large-scale production of fish skin mucous gland bioreactor and efficient application.

公开(公告)号：US10701907B2

公开(公告)日：2020-07-07

申请号：US15870334

申请日：2018-01-12

申请人： Institute of Hydrobiology, Chinese Academy of Sciences

发明人： Wei Hu ,  Mouyan Jiang ,  Yongming Li ,  Binbin Tao ,  Ji Chen ,  Zuoyan Zhu

IPC分类号： A01K67/027 ,  C12N15/00 ,  A01K61/17 ,  C12N15/01 ,  A61K31/565 ,  A61D19/02 ,  A01K61/95 ,  A01K61/80 ,  A61K31/167

摘要： Method for producing YY super-male and XY physiological female common carps, with which YY-chromosome super-male common carp can be cultivated and androgenetic YY super-male common carp is produced, where microsatellite markers are used for paternity testing and test crossing confirmation. The YY common carp is crossed with normal female common carp to produce the progenies of only male common carp, and without sex identification, the juvenile male common carp with known sex are subjected to artificial sex reversal to produce XY physiological female common carp.

公开(公告)号：US09187764B2

公开(公告)日：2015-11-17

申请号：US14247145

申请日：2014-04-07

申请人： Institute of Hydrobiology, Chinese Academy of Sciences

发明人： Wei Hu ,  Yunsheng Zhang ,  Jun Dai ,  Zuoyan Zhu

IPC分类号： C12N15/85 ,  A01K67/027

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/058 ,  A01K2217/15 ,  A01K2227/40 ,  A01K2267/02 ,  C07K14/461 ,  C12N2830/002

摘要： The invention discloses a method for controlling fish reproduction, the method comprises the following steps of: A, constructing a recombinant gene CMV-eGFP-SV40-CMV-Ga14-SV40 and establishing a GAL4 transgenic zebrafish line; B, constructing a recombinant gene CMV-RFP-SV40-UAS-antisense dnd and establishing a uas-antisensednd transgenic zebrafish line; and C, hybridizing by taking homozygous transgenic fish of the two lines as parents, and the hybrid generation fish was sterility. The method of the present invention takes a strategy that two fertilizable lines hybridized but hybrid generation is sterility, establishing a novel method with a universal meaning for controlling fish fertility; The method solves the contradiction of the reproductively sterility of fish and the hereditability of characters in a reproduction operation research and can be effectively applied to cultivation of new fish specie and population control.

摘要翻译： 本发明公开了一种鱼繁殖控制方法，该方法包括：构建重组基因CMV-eGFP-SV40-CMV-Ga14-SV40，建立GAL4转基因斑马鱼系; B，构建重组基因CMV-RFP-SV40-UAS反义dnd，建立uas-antensednd转基因斑马鱼系; 和C，通过以两条线的纯合转基因鱼作为亲本杂交，并且杂交代鱼不育。 本发明的方法采取两条可施肥的杂交品系，但是杂交代为不育的策略，建立了一种具有控制鱼类生育能力的普遍意义的新方法; 该方法解决了鱼类繁殖不育与繁殖作业研究中人物遗传性的矛盾，可有效应用于新种鱼种群和群体控制的培育。