公开(公告)号：US11198846B2

公开(公告)日：2021-12-14

申请号：US16611844

申请日：2018-05-11

申请人： Institute of Hydrobiology, Chinese Academy of Sciences

发明人： Guitang Wang ,  Weishan Zhao ,  Ming Li ,  Shangong Wu ,  Hong Zou ,  Wenxiang Li ,  Fan Xiong

IPC分类号： A01K67/033 ,  C12N1/00 ,  C12N1/10 ,  C12N5/00

摘要： A medium for culturing Balantidium ctenopharyngodoni in vitro, method for preparing the medium and method for culture in vitro are provided, which belongs to technical field of in vitro culture of intestinal protozoa. The formulation of the culture medium includes: 100 ml of Ringer's solution, 0.5 g of yeast extract, 1.0 g of proteose peptone, 3-6 ml of fetal bovine serum, 6-10 ml of horse serum, 300-500 μl of Bacillus licheniformis suspension, 200-300 mg of aseptic starch. Culture steps include: inoculating collected Balantidium ctenopharyngodoni into a prepared medium, filling with nitrogen and sealing, culturing at 15° C. for 48-72 hours, then transferring to another fresh medium, cycling back and forth, proliferating the Balantidium ctenopharyngodoni continuously. Balantidium ctenopharyngodoni are capable of achieving cell division and proliferation in the culture medium, and lays foundation for the physiological and experimental ecology research of the Balantidium ctenopharyngodoni.

公开(公告)号：US20210144977A1

公开(公告)日：2021-05-20

申请号：US16611844

申请日：2018-05-11

申请人： Institute of Hydrobiology,Chinese Academy of Sciences

发明人： Guitang Wang ,  Weishan Zhao ,  Ming Li ,  Shangong Wu ,  Hong Zou ,  Wenxiang Li ,  Fan Xiong

IPC分类号： A01K67/033 ,  C12N5/00

摘要： A medium for culturing Balantidium ctenopharyngodoni in vitro, method for preparing the medium and method for culture in vitro are provided, which belongs to technical field of in vitro culture of intestinal protozoa. The formulation of the culture medium includes: 100 ml of Ringer's solution, 0.5 g of yeast extract, 1.0 g of proteose peptone, 3-6 ml of fetal bovine serum, 6-10 ml of horse serum, 300-500 μl of Bacillus licheniformis suspension, 200-300 mg of aseptic starch. Culture steps include: inoculating collected Balantidium ctenopharyngodoni into a prepared medium, filling with nitrogen and sealing, culturing at 15° C. for 48-72 hours, then transferring to another fresh medium, cycling back and forth, proliferating the Balantidium ctenopharyngodoni continuously. Balantidium ctenopharyngodoni are capable of achieving cell division and proliferation in the culture medium, and lays foundation for the physiological and experimental ecology research of the Balantidium ctenopharyngodoni.