公开(公告)号：US20170211077A1

公开(公告)日：2017-07-27

申请号：US15143493

申请日：2016-04-29

申请人： Intact Genomics

发明人： Cheng-Cang Charles Wu

IPC分类号： C12N15/80 ,  C12N15/52 ,  C12N15/10 ,  C12N15/69

CPC分类号： C12N15/80 ,  C12N15/1082 ,  C12N15/1093 ,  C12N15/52 ,  C12N15/69 ,  C12N2800/20 ,  C12N2800/70 ,  C12N2820/002 ,  C12N2820/10 ,  C12N2820/55 ,  C12Q2525/191

摘要： Fungal artificial chromosome (FAC) vectors are disclosed. A vector can be replicated in a bacterial or a fungal host, and can comprise an insert of heterologous DNA up to about 500 kilobases. A vector can be used for cloning and expressing a secondary metabolite (SM) gene cluster. An insert sequence can be modified by homologous recombination. A vector can be a plasmid comprising bacterial and fungal origins of replication, as well as bacterial and fungal selection marker genes. Also disclosed are vectors that can be integrated into a fungal genome, and dual function vectors which can be replicated in a bacterial or a fungal host and can also be integrated into a fungal genome. Also disclosed are methods of generating plasmid libraries including vectors comprising intact SM gene clusters.