公开(公告)号：US20240344057A1

公开(公告)日：2024-10-17

申请号：US18647281

申请日：2024-04-26

Applicant: Twist Bioscience Corporation

Inventor： Jeremy LACKEY ,  David DODD

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/1093 ,  C12N15/102 ,  C12N15/113 ,  C12N2310/315 ,  C12N2320/33

Abstract: Disclosed herein are methods and compositions comprising a polymerase and a phosphorylated nucleoside, wherein the polymerase and the nucleoside are covalently linked by a cleavable linker at the terminal phosphate group. Further disclosed herein are enzymatic polynucleotide synthesis using polymerase and nucleotide conjugation strategies.

公开(公告)号：US20240336967A1

公开(公告)日：2024-10-10

申请号：US18377068

申请日：2023-10-05

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Mirna Jarosz ,  Paul Hardenbol ,  Michael Schnall-Levin ,  Kevin Ness ,  Serge Saxonov

IPC: C12Q1/6874 ,  C12N15/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/683

CPC classification number: C12Q1/6874 ,  C12N15/00 ,  C12N15/10 ,  C12N15/1093 ,  C12Q1/683 ,  C12Q1/6806 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/179

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing. In some cases, this disclosure provides methods for the generation of polynucleotide barcode libraries, and for the attachment of such polynucleotides to target polynucleotides.

公开(公告)号：US20240309362A1

公开(公告)日：2024-09-19

申请号：US18577037

申请日：2022-07-07

Applicant: ARC BIO, LLC

Inventor： Stephane B. GOURGUECHON ,  Manuel Bernhard KRISPIN

IPC: C12N15/10 ,  C12N9/22 ,  C12N15/11

CPC classification number: C12N15/1093 ,  C12N9/22 ,  C12N15/11 ,  C12N2310/20

Abstract: The present invention provides methods for selectively depleting unwanted sequences from a pool of nucleic acids, including (1) methods that can be used to deplete an unwanted sequence from a single-stranded DNA library, and (2) methods that can be used to deplete an unwanted non-polyadenylated RNA from a pool of single-stranded RNA molecules. By depleting the unwanted sequences, the methods enrich a sample for sequences of interest.

公开(公告)号：US12084652B2

公开(公告)日：2024-09-10

申请号：US17705115

申请日：2022-03-25

Applicant: Viome Life Sciences, Inc.

Inventor： Momchilo Vuyisich ,  Andrew Hatch ,  Ryan Toma ,  Brittany Twibell ,  James Horne ,  Kinga Canfield

IPC: C12N15/10 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q1/689 ,  C40B40/06 ,  C40B40/08

CPC classification number: C12N15/1068 ,  C12N15/1065 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6844 ,  C12Q1/689 ,  C40B40/06 ,  C40B40/08 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12Q2527/143

Abstract: Provided herein are methods and composition for processing samples that contain nucleic acids, or cells containing nucleic acids, of a microbiome, using amounts of primers within a range of mole values and rounds of polymerase chain reaction (PCR) within a range of numbers of rounds.

公开(公告)号：US20240279647A1

公开(公告)日：2024-08-22

申请号：US18016857

申请日：2021-11-18

Applicant: Nanodigmbio?Nanjing?Biotechnology Co., LTD

Inventor： Biao WANG ,  Yugang HU ,  Qiang WU

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6855

CPC classification number: C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6855

Abstract: The present application provides a library construction element, a kit and a library construction method compatible with double sequencing platforms. The library construction method comprises: performing library construction on target samples using primers or adaptors with a 5′ phosphorylation modification to obtain a linear amplification library with a 5′ phosphorylation modification, that is a linear library suitable for Illumina sequencing platform; or further cyclizing the linear amplification library with a 5′ phosphorylation modification to obtain a cyclization library suitable for the MGI sequencing platform.

公开(公告)号：US12037707B2

公开(公告)日：2024-07-16

申请号：US17045120

申请日：2019-04-05

Applicant: Massachusetts Eye and Ear Infirmary ,  The Schepens Eye Research Institute, Inc.

Inventor： Luk H. Vandenberghe ,  Eric Zinn ,  Heikki Turunen

IPC: C40B50/06 ,  C12N15/10 ,  C12Q1/6897 ,  C40B40/08

CPC classification number: C40B50/06 ,  C12N15/1093 ,  C12Q1/6897 ,  C40B40/08

Abstract: This disclosure describes compositions, methods, and systems for constructing defined variation in a contiguous functional genetic unit in association with a unique sequence identifier (“a barcode”) in a combinatorial manner.

公开(公告)号：US11999950B2

公开(公告)日：2024-06-04

申请号：US16871754

申请日：2020-05-11

Applicant: The Chinese University of Hong Kong

Inventor： Kwan Chee Chan ,  Wanxia Gai ,  Yuk-Ming Dennis Lo

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1093 ,  C12N15/1031 ,  C12Q1/68 ,  C12Q2521/101 ,  C12Q2533/101 ,  C12Q2535/125 ,  C12N15/1075 ,  C12Q2521/101 ,  C12Q2533/101 ,  C12N15/1075 ,  C12Q2521/101 ,  C12Q2533/101 ,  C12Q2535/125

Abstract: Particular forward and reverse primers may be used to link distant regions of the same large DNA molecule into a smaller DNA molecule. A reverse primer R1 can have a first portion complementary to an ending sequence of region A and can have a second portion having an overlapping sequence. A forward primer F2 can have a first portion complementary to a starting sequence of region B, where the forward primer includes a complementary overlapping sequence (e.g., the same first portion or a second portion) that is complementary to the overlapping sequence. The first portion of F2 may be the entire primer. The smaller DNA molecules can be used to determine haplotypes of regions. Kits including the particular forward and reverse primers are also described.

公开(公告)号：US20240141332A1

公开(公告)日：2024-05-02

申请号：US18051966

申请日：2022-11-02

Applicant: Illumina, Inc.

Inventor： Xi-Jun Chen ,  Tarun Khurana

IPC: C12N15/10

CPC classification number: C12N15/1093

Abstract: Systems, methods and compositions provided herein relate to the preparation of nucleic acid libraries. Some embodiments include the preparation of nucleic acid libraries by ligation of single-stranded nucleic acids.

公开(公告)号：US11970697B2

公开(公告)日：2024-04-30

申请号：US17504358

申请日：2021-10-18

Applicant: Twist Bioscience Corporation

Inventor： Jeremy Lackey ,  David Dodd

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/1093 ,  C12N15/102 ,  C12N15/113 ,  C12N2310/315 ,  C12N2320/33

Abstract: Disclosed herein are methods and compositions comprising a polymerase and a phosphorylated nucleoside, wherein the polymerase and the nucleoside are covalently linked by a cleavable linker at the terminal phosphate group. Further disclosed herein are enzymatic polynucleotide synthesis using polymerase and nucleotide conjugation strategies.

公开(公告)号：US11959075B2

公开(公告)日：2024-04-16

申请号：US18184967

申请日：2023-03-16

Applicant: President and Fellows of Harvard College

Inventor： Xiaowei Zhuang ,  Kok-Hao Chen ,  Alistair Boettiger ,  Jeffrey R. Moffitt ,  Siyuan Wang

IPC: C12N15/10 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6869 ,  G06N7/01 ,  G16B25/00 ,  G16B25/20 ,  G16B40/10 ,  G16C20/10 ,  C12Q1/6816 ,  G16B30/00 ,  G16B40/00

CPC classification number: C12N15/1065 ,  C07H21/02 ,  C07H21/04 ,  C12N15/10 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6837 ,  G16B25/00 ,  G16B25/20 ,  G16B40/10 ,  G16C20/10 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6869 ,  G06N7/01 ,  G16B30/00 ,  G16B40/00 ,  C12Q1/6806 ,  C12Q2521/107 ,  C12Q2525/143 ,  C12Q2525/179 ,  C12Q2537/143 ,  C12Q2565/514 ,  C12Q1/6816 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2563/179 ,  C12Q1/6816 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2563/179 ,  C12Q2565/102

Abstract: The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.