公开(公告)号：US20090212776A1

公开(公告)日：2009-08-27

申请号：US12371963

申请日：2009-02-17

申请人： Isao Kitagawa ,  Michiya Okada ,  Kazuo Saitoh

发明人： Isao Kitagawa ,  Michiya Okada ,  Kazuo Saitoh

IPC分类号： G01R33/30 ,  G01R33/465 ,  G01R33/483

CPC分类号： G01R33/307 ,  G01N24/087 ,  G01N24/088 ,  G01R33/465

摘要： An NMR measurement apparatus adopts a circulation flow scheme using a sample solution containing a high molecular compound representing a measuring object and a low molecular compound solution containing a low molecular compound representing a ligand. The measurement apparatus comprises a mixing filter 32 for mixing the sample solution and the low molecular compound solution, a separation filter 34 for performing separation therebetween, a flow channel 1 through which the sample solution drained out of the separation filter 34 is injected to the mixing filter 32, a flow channel 2 through which the low molecular compound solution drained out of the separation filter 34 is injected to the mixing filter 32, and a flow channel 3 through which the mixture solution drained out of the mixing filter 32 is injected to the separation filter 34 by way of a reservoir 10.

摘要翻译： NMR测定装置采用使用含有表示测定对象的高分子化合物的样品溶液和含有表示配体的低分子化合物的低分子化合物溶液的循环流程图。 测量装置包括用于混合样品溶液和低分子化合物溶液的混合过滤器32，用于在其间进行分离的分离过滤器34，将从分离过滤器34排出的样品溶液注入混合物的流动通道1 过滤器32将从分离过滤器34排出的低分子化合物溶液注入到流通道2中，并将混合溶液排出混合过滤器32的流路3注入到混合过滤器32中， 分离过滤器34通过储存器10。

公开(公告)号：US07898256B2

公开(公告)日：2011-03-01

申请号：US12371963

申请日：2009-02-17

申请人： Isao Kitagawa ,  Michiya Okada ,  Kazuo Saitoh

发明人： Isao Kitagawa ,  Michiya Okada ,  Kazuo Saitoh

IPC分类号： G01V3/00

CPC分类号： G01R33/307 ,  G01N24/087 ,  G01N24/088 ,  G01R33/465

摘要： An NMR measurement apparatus adopts a circulation flow scheme using a sample solution containing a high molecular compound representing a measuring object and a low molecular compound solution containing a low molecular compound representing a ligand. The measurement apparatus comprises a mixing filter 32 for mixing the sample solution and the low molecular compound solution, a separation filter 34 for performing separation therebetween, a flow channel 1 through which the sample solution drained out of the separation filter 34 is injected to the mixing filter 32, a flow channel 2 through which the low molecular compound solution drained out of the separation filter 34 is injected to the mixing filter 32, and a flow channel 3 through which the mixture solution drained out of the mixing filter 32 is injected to the separation filter 34 by way of a reservoir 10.

摘要翻译： NMR测定装置采用使用含有表示测定对象的高分子化合物的样品溶液和含有表示配体的低分子化合物的低分子化合物溶液的循环流程图。 测量装置包括用于混合样品溶液和低分子化合物溶液的混合过滤器32，用于在其间进行分离的分离过滤器34，将从分离过滤器34排出的样品溶液注入混合物的流动通道1 过滤器32将从分离过滤器34排出的低分子化合物溶液注入到流通道2中，并将混合溶液排出混合过滤器32的流路3注入到混合过滤器32中， 分离过滤器34通过储存器10。

公开(公告)号：US20080290872A1

公开(公告)日：2008-11-27

申请号：US12153349

申请日：2008-05-16

申请人： Isao Kitagawa ,  Michiya Okada ,  Kazuo Saitoh

发明人： Isao Kitagawa ,  Michiya Okada ,  Kazuo Saitoh

IPC分类号： G01R33/30

CPC分类号： G01R33/307

摘要： A sample tube is used to ensure uniformity in a static magnetic field and uniformity in electromagnetic wave irradiation for NMR measurement for continuous sample injection. The sample tube is formed of a signal detecting tube having a length lying between 80% and 100% of the length of an antenna, the signal detecting tube accommodating a sample at the position of the antenna; first and second joint tubes each having an outside diameter equal to the outside diameter of the signal detecting tube and having an inside diameter smaller than the inside diameter of the signal detecting tube; and injection and ejection supporting tubes each having an inside diameter smaller than the inside diameter of the signal detecting tube. The first and second joint tubes have magnetic susceptibility matched to or brought close to that of a sample solvent.

摘要翻译： 使用样品管来确保静电磁场的均匀性和用于连续样品注入的NMR测量的电磁波照射的均匀性。 样品管由长度位于天线长度的80％至100％之间的信号检测管形成，信号检测管在天线的位置容纳样品; 第一和第二接头管的外径等于信号检测管的外径，内径小于信号检测管的内径; 以及各自具有小于信号检测管的内径的内径的注射和喷射支撑管。 第一和第二接合管具有与样品溶剂匹配或接近的磁化率。

公开(公告)号：US20070273381A1

公开(公告)日：2007-11-29

申请号：US11798015

申请日：2007-05-09

申请人： Isao Kitagawa ,  Michiya Okada

发明人： Isao Kitagawa ,  Michiya Okada

IPC分类号： G01V3/00

CPC分类号： G01R33/307 ,  G01N24/08 ,  G01N24/087 ,  G01N24/088 ,  G01R33/465

摘要： Briefly, the present invention provides an apparatus and a method for performing a nuclear magnetic resonance (NMR) measurement on a sample solution containing small molecules and large molecules such as proteins, and provides an apparatus and a method for repeatedly performing the NMR measurement while stably controlling concentration conditions in a sample and changing a concentration of small molecules.A sample solution containing small molecules and large molecules is circularly transferred between a vessel equipped with a nuclear magnetic resonance probe and a control section in which injection and filtration of small molecules are performed. By transferring the sample solution between them, the NMR measurement can be performed while maintaining a concentration of large molecules to be constant and increasing or reducing a concentration of small molecules so that a concentration ratio of the small molecules relative to the large molecules is changed, the two types of molecules being present in the sample solution.

摘要翻译： 简而言之，本发明提供了对含有小分子和大分子如蛋白质的样品溶液进行核磁共振（NMR）测量的装置和方法，并且提供了一种在稳定地重复进行NMR测量的装置和方法 控制样品中的浓度条件和改变小分子的浓度。 含有小分子和大分子的样品溶液在配备有核磁共振探针的容器和其中进行小分子的注射和过滤的对照部分之间循环转移。 通过在它们之间转移样品溶液，可以在保持大分子浓度恒定并增加或降低小分子浓度的同时进行NMR测量，从而改变小分子相对于大分子的浓度比， 存在于样品溶液中的两种类型的分子。

公开(公告)号：US07492157B2

公开(公告)日：2009-02-17

申请号：US11940398

申请日：2007-11-15

申请人： Isao Kitagawa ,  Michiya Okada

发明人： Isao Kitagawa ,  Michiya Okada

IPC分类号： G01V3/00

CPC分类号： G01R33/307

摘要： The present invention makes it possible to measure a plurality of samples under a certain concentration condition of the small molecule by the circulation transfer of a small molecule solution through a small molecule concentration control portion for injecting, discharging or feeding the small molecule solution, a mixing filter portion for mixing a solution (sample) containing the large molecule sample with the small molecule solution, a container set in a nuclear magnetic resonance probe, a separating filter portion for separating the mixture into the sample and the small molecule solution, and the small molecule concentration control portion; injecting the sample from the mixing filter portion; holding the sample in a measurement portion in the container; and discharging the sample from the separating filter portion after completion of the NMR measurement.

摘要翻译： 本发明使得可以通过小分子溶液的循环转移通过小分子浓度控制部分小分子溶液的注入，排出或进料来测量小分子在一定浓度条件下的多个样品，混合 用于将含有大分子样品的溶液（样品）与小分子溶液混合的过滤器部分，设置在核磁共振探针中的容器，用于将混合物分离成样品的分离过滤部分和小分子溶液，以及小分子溶液 分子浓度控制部分; 从混合过滤器部分注入样品; 将样品保持在容器中的测量部分中; 并在NMR测量完成之后从分离过滤器部分排出样品。

公开(公告)号：US20090146661A1

公开(公告)日：2009-06-11

申请号：US12366999

申请日：2009-02-06

申请人： Isao Kitagawa ,  Michiya Okada

发明人： Isao Kitagawa ,  Michiya Okada

IPC分类号： G01R33/30

CPC分类号： G01R33/307

摘要： An apparatus for nuclear magnetic resonance measurement includes a measurement portion having a magnet for applying a magnetic field to a sample, a bore within the magnet, a nuclear magnetic resonance probe disposed in the bore, and a container for retaining the sample therein; a mixing filter portion for mixing a small molecule solution with a sample solution; a separating filter portion; a small molecule concentration controlling portion; a transmitter/receiver system; a unit for carrying out circulation solution transfer; a unit for injecting the small molecule solution; a unit for controlling the small molecule concentration; a unit for injecting the sample solution; a unit for holding the sample solution in the measurement portion; a unit for discharging the sample solution; and a unit for carrying out nuclear magnetic resonance measurement of the sample solution.

摘要翻译： 用于核磁共振测量的装置包括具有用于向样品施加磁场的磁体的测量部分，磁体内的孔，设置在孔中的核磁共振探针和用于将样品保持在其中的容器; 用于将小分子溶液与样品溶液混合的混合过滤器部分; 分离过滤器部分; 小分子浓度控制部分; 发射机/接收机系统; 用于进行循环溶液转移的单元; 用于注射小分子溶液的单元; 用于控制小分子浓度的单元; 用于注射样品溶液的单元; 用于将样品溶液保持在测量部分中的单元; 用于排出样品溶液的单元; 以及用于进行样品溶液的核磁共振测量的单元。

公开(公告)号：US07449890B2

公开(公告)日：2008-11-11

申请号：US11798015

申请日：2007-05-09

申请人： Isao Kitagawa ,  Michiya Okada

发明人： Isao Kitagawa ,  Michiya Okada

IPC分类号： G01V3/00

CPC分类号： G01R33/307 ,  G01N24/08 ,  G01N24/087 ,  G01N24/088 ,  G01R33/465

摘要： Briefly, the present invention provides an apparatus and a method for performing a nuclear magnetic resonance (NMR) measurement on a sample solution containing small molecules and large molecules such as proteins, and provides an apparatus and a method for repeatedly performing the NMR measurement while stably controlling concentration conditions in a sample and changing a concentration of small molecules.A sample solution containing small molecules and large molecules is circularly transferred between a vessel equipped with a nuclear magnetic resonance probe and a control section in which injection and filtration of small molecules are performed. By transferring the sample solution between them, the NMR measurement can be performed while maintaining a concentration of large molecules to be constant and increasing or reducing a concentration of small molecules so that a concentration ratio of the small molecules relative to the large molecules is changed, the two types of molecules being present in the sample solution.

摘要翻译： 简而言之，本发明提供了对含有小分子和大分子如蛋白质的样品溶液进行核磁共振（NMR）测量的装置和方法，并且提供了一种在稳定地重复进行NMR测量的装置和方法 控制样品中的浓度条件和改变小分子的浓度。 含有小分子和大分子的样品溶液在配备有核磁共振探针的容器和其中进行小分子的注射和过滤的对照部分之间循环转移。 通过在它们之间转移样品溶液，可以在保持大分子浓度恒定并增加或降低小分子浓度的同时进行NMR测量，从而改变小分子相对于大分子的浓度比， 存在于样品溶液中的两种类型的分子。

公开(公告)号：US20080174312A1

公开(公告)日：2008-07-24

申请号：US11940398

申请日：2007-11-15

申请人： Isao Kitagawa ,  Michiya Okada

发明人： Isao Kitagawa ,  Michiya Okada

IPC分类号： G01R33/30

CPC分类号： G01R33/307

摘要： The present invention makes it possible to measure a plurality of samples under a certain concentration condition of the small molecule by the circulation transfer of a small molecule solution through a small molecule concentration control portion for injecting, discharging or feeding the small molecule solution, a mixing filter portion for mixing a solution (sample) containing the large molecule sample with the small molecule solution, a container set in a nuclear magnetic resonance probe, a separating filter portion for separating the mixture into the sample and the small molecule solution, and the small molecule concentration control portion; injecting the sample from the mixing filter portion; holding the sample in a measurement portion in the container; and discharging the sample from the separating filter portion after completion of the NMR measurement.

摘要翻译： 本发明使得可以通过小分子溶液的循环转移通过小分子浓度控制部分小分子溶液的注入，排出或进料来测量小分子在一定浓度条件下的多个样品，混合 用于将含有大分子样品的溶液（样品）与小分子溶液混合的过滤器部分，设置在核磁共振探针中的容器，用于将混合物分离成样品的分离过滤部分和小分子溶液，以及小分子溶液 分子浓度控制部分; 从混合过滤器部分注入样品; 将样品保持在容器中的测量部分中; 并在NMR测量完成之后从分离过滤器部分排出样品。

公开(公告)号：US20080231277A1

公开(公告)日：2008-09-25

申请号：US12010292

申请日：2008-01-23

申请人： Hiroyuki Yamamoto ,  Kazuo Saitoh ,  Haruhiro Hasegawa ,  Masaya Takahashi ,  Michiya Okada

发明人： Hiroyuki Yamamoto ,  Kazuo Saitoh ,  Haruhiro Hasegawa ,  Masaya Takahashi ,  Michiya Okada

IPC分类号： G01R33/31

CPC分类号： G01R33/34076 ,  G01R33/30 ,  G01R33/307 ,  G01R33/34007 ,  G01R33/34023 ,  G01R33/34046 ,  G01R33/34053 ,  G01R33/34069 ,  G01R33/56536

摘要： The present invention provides a highly-sensitive nuclear magnetic resonance (NMR) spectrometer which achieves a high Q factor using a superconductor, and concurrently which is provided with a probe antenna maintaining the magnetic homogeneity of the static magnetic field in a sample space. An antenna coil is fabricated by using a wire having a superconducting layer formed on the surface of a metal wire.

摘要翻译： 本发明提供使用超导体实现高Q因子的高灵敏度核磁共振（NMR）光谱仪，并且同时具有保持样品空间中的静磁场的磁均匀性的探针天线。 通过使用在金属线的表面上形成有超导层的导线来制造天线线圈。

公开(公告)号：US07173424B2

公开(公告)日：2007-02-06

申请号：US11196279

申请日：2005-08-04

申请人： Kazuo Saitoh ,  Haruhiro Hasegawa ,  Hiroyuki Yamamoto ,  Hisaaki Ochi ,  Tsuyoshi Wakuda ,  Michiya Okada

发明人： Kazuo Saitoh ,  Haruhiro Hasegawa ,  Hiroyuki Yamamoto ,  Hisaaki Ochi ,  Tsuyoshi Wakuda ,  Michiya Okada

IPC分类号： G01V3/00

CPC分类号： G01R33/34053 ,  G01R33/34007 ,  G01R33/34023 ,  G01R33/34069 ,  G01R33/34092

摘要： A configuration of an NMR apparatus is provided. In the NMR apparatus, a magnetic field space of higher uniformity is generated by split superconducting magnets. At the same time, it is provided with a cryo probe excellent in cooling capability and sensitivity between two superconducting solenoid coils constructed in very close proximity to each other. For this purpose, a probe coil provided between the two superconducting solenoid coils is so constructed that the following is implemented: a certain distance is ensured between a substrate with a coil formed thereon and another, and the substrates and spacer substrates for cooling are alternately laminated. The spacer substrates are cooled by a cold head of sapphire. When the probe coil is inserted in the same direction as a sample tube (direction perpendicular to the static magnetic field), the spacer substrates cannot be coupled directly by the cold head of sapphire. Therefore, they are cooled through a fixed substrate for thermal conduction of sapphire coupled with the cold head.

摘要翻译： 提供了NMR装置的结构。 在NMR装置中，通过分体超导磁体产生更高均匀性的磁场空间。 同时，它提供了一种低温探针，该低温探针具有非常接近的两个超导螺线管线圈之间的冷却能力和灵敏度。 为此，设置在两个超导螺线管线圈之间的探针线圈被构造成使得实现以下功能：在其上形成有线圈的基板和另一个之间确保一定距离，并且用于冷却的基板和间隔基板交替层压 。 间隔基板被蓝宝石的冷头冷却。 当探针线圈沿着与样品管相同的方向（垂直于静磁场的方向）插入时，间隔基板不能由蓝宝石的冷头直接耦合。 因此，它们通过固定的基板进行冷却，用于与冷头连接的蓝宝石的热传导。