公开(公告)号：US09057065B2

公开(公告)日：2015-06-16

申请号：US14021260

申请日：2013-09-09

Applicant: Japan Science and Technology Agency

Inventor： Tan Inoue ,  Hirohide Saito ,  Yusho Kato ,  Kenichi Yoshikawa ,  Ayako Yamada ,  Toru Yamanaka

IPC: C07H21/04 ,  C12N15/11 ,  C12N15/67

CPC classification number: C12N15/11 ,  C12N15/111 ,  C12N15/67 ,  C12N2310/141 ,  C12N2320/00 ,  C12N2330/10

Abstract: An object of the present invention is to construct an mRNA which specifically responds to a short RNA sequence and can activate, repress, and regulate the translation of the desired gene, and to construct an artificial cell model system using a liposome comprising the mRNA and a cell-free translational system encapsulated therein. The present invention provides: an mRNA comprising a target RNA-binding site located immediately 5′ to the ribosome-binding site, and a nucleotide sequence located 5′ to the target RNA-binding site, the nucleotide sequence being complementary to the ribosome-binding site; an mRNA comprising a small RNA-binding site located 3′ to the start codon, and a nucleotide sequence located 3′ to the small RNA-binding site, the nucleotide sequence encoding a protein; and a liposome comprising any of these mRNAs encapsulated therein.

Abstract translation: 本发明的目的是构建对短RNA序列具有特异性响应并可以活化，抑制和调节所需基因翻译的mRNA，并构建使用包含mRNA和脂质体的脂质体的人造细胞模型系统 封装在其中的无细胞翻译系统。 本发明提供：包含位于核糖体结合位点5'处的靶RNA结合位点和位于靶RNA结合位点5'的核苷酸序列的mRNA，所述核苷酸序列与核糖体结合位置互补 现场; 包含位于起始密码子3'的小RNA结合位点和位于小RNA结合位点3'的核苷酸序列的mRNA，编码蛋白质的核苷酸序列; 和包含其中包含的任何这些mRNA的脂质体。

公开(公告)号：US20140030707A1

公开(公告)日：2014-01-30

申请号：US14021260

申请日：2013-09-09

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Tan Inoue ,  Hirohide Saito ,  Yusho Kato ,  Kenichi Yoshikawa ,  Ayako Yamada ,  Toru Yamanaka

IPC: C12N15/11

CPC classification number: C12N15/11 ,  C12N15/111 ,  C12N15/67 ,  C12N2310/141 ,  C12N2320/00 ,  C12N2330/10

Abstract: An object of the present invention is to construct an mRNA which specifically responds to a short RNA sequence and can activate, repress, and regulate the translation of the desired gene, and to construct an artificial cell model system using a liposome comprising the mRNA and a cell-free translational system encapsulated therein. The present invention provides: an mRNA comprising a target RNA-binding site located immediately 5′ to the ribosome-binding site, and a nucleotide sequence located 5′ to the target RNA-binding site, the nucleotide sequence being complementary to the ribosome-binding site; an mRNA comprising a small RNA-binding site located 3′ to the start codon, and a nucleotide sequence located 3′ to the small RNA-binding site, the nucleotide sequence encoding a protein; and a liposome comprising any of these mRNAs encapsulated therein.

Abstract translation: 本发明的目的是构建对短RNA序列具有特异性响应并可以活化，抑制和调节所需基因翻译的mRNA，并构建使用包含mRNA和脂质体的脂质体的人造细胞模型系统 封装在其中的无细胞翻译系统。 本发明提供：包含位于核糖体结合位点5'处的靶RNA结合位点和位于靶RNA结合位点5'的核苷酸序列的mRNA，所述核苷酸序列与核糖体结合位置互补 现场; 包含位于起始密码子3'的小RNA结合位点和位于小RNA结合位点3'的核苷酸序列的mRNA，编码蛋白质的核苷酸序列; 和包含其中包含的任何这些mRNA的脂质体。