公开(公告)号：US20200140830A1

公开(公告)日：2020-05-07

申请号：US16620525

申请日：2018-04-13

申请人： JIANGSU AOSAIKANG PHARMACEUTICAL CO., LTD. ,  EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

发明人： Huilei YU ,  Yan ZHANG ,  Jianhe XU ,  Qian ZHAO ,  Jiang PAN ,  Feng LIU ,  Guoqiang LIN

IPC分类号： C12N9/02 ,  C12P17/16 ,  C12P17/10 ,  C12P11/00

摘要： A Bradyrhizobium monooxygenase, a gene for encoding the monooxygenase, a recombinant expression vector comprising the gene and a recombinant transformant, a method of preparing the monooxygenase by the recombinant expression transformant, and a method of preparing an optically pure chiral sulfoxide by the monooxygenase, in particular to a method of preparing prazole drugs by means of catalyzing the asymmetric oxidation of thioether, a prazole precursor. As compared with other methods of preparing an optically pure sulfoxide, the product produced by the monooxygenase of the present invention as a catalyst has high optical purity, avoids the generation of the byproduct sulfone, and has advantages of mild reaction conditions, simple and convenient operations, easy amplification, etc.