公开(公告)号：US20230212654A1

公开(公告)日：2023-07-06

申请号：US17797393

申请日：2021-02-07

Applicant: JIANGSU UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Wei SHEN ,  Jun SUN ,  Tong QI ,  Sheng TANG ,  Chang SONG

IPC: C12Q1/6834

CPC classification number: C12Q1/6834 ,  C12Q2525/207 ,  C12Q2563/107 ,  C12Q2565/137

Abstract: A liquid chromatography method for simultaneously detecting multiple microRNAs based on a duplex-specific nuclease (DSN) cyclic amplification technology comprises the following steps: designing a fluorophore-modified single-stranded DNA probe according to a target microRNA to be detected and loading the probe onto a surface of a streptavidin-coated magnetic bead (MB) to serve as a detection probe; adding a target microRNA sample to be detected and DSN to the detection probe, fully mixing the same, and incubating the mixture; after the incubation, completely removing the magnetic bead and the unreacted DNA probe to obtain a separated solution; and injecting the separated solution into a high-performance liquid chromatography system for separation and quantification.