公开(公告)号：US20230212654A1

公开(公告)日：2023-07-06

申请号：US17797393

申请日：2021-02-07

Applicant: JIANGSU UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Wei SHEN ,  Jun SUN ,  Tong QI ,  Sheng TANG ,  Chang SONG

IPC: C12Q1/6834

CPC classification number: C12Q1/6834 ,  C12Q2525/207 ,  C12Q2563/107 ,  C12Q2565/137

Abstract: A liquid chromatography method for simultaneously detecting multiple microRNAs based on a duplex-specific nuclease (DSN) cyclic amplification technology comprises the following steps: designing a fluorophore-modified single-stranded DNA probe according to a target microRNA to be detected and loading the probe onto a surface of a streptavidin-coated magnetic bead (MB) to serve as a detection probe; adding a target microRNA sample to be detected and DSN to the detection probe, fully mixing the same, and incubating the mixture; after the incubation, completely removing the magnetic bead and the unreacted DNA probe to obtain a separated solution; and injecting the separated solution into a high-performance liquid chromatography system for separation and quantification.

公开(公告)号：US20220155222A1

公开(公告)日：2022-05-19

申请号：US17440189

申请日：2020-02-28

Applicant: JIANGSU UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Sheng TANG ,  Wei SHEN ,  Tong QI ,  Mengchan XU ,  Mengyuan XU ,  Anni ZHU

IPC: G01N21/33 ,  G01N1/22 ,  G01N33/04

Abstract: The present invention provides a method for determining hydrogen sulfide (H2S) by headspace single-drop liquid phase microextraction and intelligent device colorimetry, which comprises: taking a silver-gold core-shell triangular nanosheet (Ag@Au TNS) as a nanodetection probe, in combination with an analysis method of headspace single-drop microextraction (HS-SDME), specifically extracting H2S volatilized from a sample to be detected by the nanodetection probe, and detecting H2S in the extracted sample with the help of the photographing function of an intelligent device and a color picking software. Compared with the prior art, the present invention adopts intelligent device colorimetry, with the limit of detection of about 65 nM and the linear range of 0.1-100 μM, and the established method can be applied to the determination of H2S in actual samples such as egg white, milk and other opaque samples, and has the advantages of few procedures, simple operation, high detection efficiency and the like.