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    Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda PL promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof
    1.
    发明授权
    Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda PL promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof 失效
    用于表达含有λPL启动子的人超氧化物歧化酶（SOD）类似物用于代替核糖体结合位点的工程化限制性位点的质粒及其使用方法

    公开(公告)号：US5162217A

    公开(公告)日：1992-11-10

    申请号：US449125

    申请日：1989-12-08

    申请人： Jacob R. Hartman ,  Amos B. Oppenheim ,  Marian Gorecki ,  Haim Aviv ,  Rachel Oren

    发明人： Jacob R. Hartman ,  Amos B. Oppenheim ,  Marian Gorecki ,  Haim Aviv ,  Rachel Oren

    IPC分类号： A23K1/165 ,  A61K38/44 ,  C07K14/61 ,  C07K14/775 ,  C12N9/02 ,  C12N15/53 ,  C12N15/69 ,  C12N15/73

    CPC分类号： C12N9/0089 ,  A23K20/168 ,  A61K38/446 ,  C07K14/61 ,  C07K14/775 ,  C12N15/69 ,  C12N15/73 ,  Y10S514/886

    摘要： An improved plasmid for the production of superoxide dismutase which upon introduction into a host containing the thermolabile repressor C.sub.I renders the host capable of effecting expression of a gene encoding superoxide dismutase. The plasmid which includes in 5' to 3' order the following: the promoter and operator P.sub.L O.sub.L from lambda bacteriophage; the N utilization site; a first restriction enzyme site permitting replacement of the ribosomal binding site which follows thereafter; a ribosomal binding site; an ATG initiation codon; a second restriction enzyme site; a gene encoding superoxide dismutase; an origin of replication and a gene associated with a selectable or identifiable phenotypic trait manifested when the plasmid is present in the host. The distance between the 3' end of P.sub.L O.sub.L and the 5' end of the N utilization site is less than about 80 base pairs. The distance between the 3' end of the N utilization site and the 5' end of the ribosomal binding site is less than about 300 base pairs.Host vector systems have been constructed from these plasmids and used to produce human superoxide dismutase and analogs thereof. Such SOD or analogs may be used to catalyze the reduction of superoxide radicals, reduce reperfusion injury, prolong the survival time of isolated organs and reduce spinal cord injury.

    摘要翻译： 用于生产超氧化物歧化酶的改良质粒，其在引入含有不耐热阻遏物CI的宿主中时，使宿主能够实现编码超氧化物歧化酶的基因的表达。 包含5'至3'的质粒命名如下：来自λ噬菌体的启动子和操纵子PLOL; N利用场所; 第一限制性内切酶位点，其允许置换随后的核糖体结合位点; 核糖体结合位点; ATG起始密码子; 第二限制酶位点; 编码超氧化物歧化酶的基因; 复制起点和当质粒存在于宿主中时显示的与可选择或可鉴定的表型性状相关的基因。 PLOL的3'端与N利用位点的5'端之间的距离小于约80个碱基对。 N利用位点的3'末端与核糖体结合位点的5'末端之间的距离小于约300个碱基对。 宿主载体系统已经由这些质粒构建并用于产生人超氧化物歧化酶及其类似物。 这种SOD或类似物可用于催化超氧自由基的减少，减少再灌注损伤，延长分离器官的存活时间并减少脊髓损伤。

    Therapeutic compositions comprising a mixture of human CuZn superoxide dismutase analogs
    3.
    发明授权
    Therapeutic compositions comprising a mixture of human CuZn superoxide dismutase analogs 失效
    包含人CuZn超氧化物歧化酶类似物的混合物的治疗组合物

    公开(公告)号：US5455029A

    公开(公告)日：1995-10-03

    申请号：US933500

    申请日：1992-08-21

    申请人： Jacob R. Hartman ,  Amos B. Oppenheim ,  Marian Gorecki ,  Haim Aviv ,  Rachel Oren

    发明人： Jacob R. Hartman ,  Amos B. Oppenheim ,  Marian Gorecki ,  Haim Aviv ,  Rachel Oren

    IPC分类号： A23K1/165 ,  A61K38/44 ,  C07K14/61 ,  C07K14/775 ,  C12N9/02 ,  C12N15/53 ,  C12N15/69 ,  C12N15/73

    CPC分类号： C12N9/0089 ,  A23K20/168 ,  A61K38/446 ,  C07K14/61 ,  C07K14/775 ,  C12N15/69 ,  C12N15/73 ,  Y10S514/886

    摘要： An improved plasmid for the production of superoxide dismutase on an analog thereof which upon introduction into a suitable host containing the thermolabile repressor C.sub.I renders the host capable of effecting expression of a gene encoding superoxide dismutase or the analog. The plasmid is a double-stranded DNA molecule which includes in 5' to 3' order the following: the promoter and operator P.sub.L O.sub.L from lambda bacteriophage; the N utilization site; a first restriction enzyme site permitting replacement of the ribosomal binding site which follows thereafter; a ribosomal binding site; an ATG initiation codon; a second restriction enzyme site; a gene encoding superoxide dismutase or the analog; an origin of replication and a gene associated with a selectable or identifiable phenotypic trait manifested when the plasmid is present in the host. The distance between the 3' end of P.sub.L O.sub.L and the 5' end of the N utilization site is less than about 80 base pairs. The distance between the 3' end of the N utilization site and the 5' end of the ribosomal binding site is less than about 300 base pairs.Host vector systems have been constructed from these plasmids and used to produce human superoxide dismutase and analogs thereof. Such SOD or analogs may be used to catalyze the reduction of superoxide radicals, reduce reperfusion injury, prolong the survival time of isolated organs and reduce spinal cord injury.

    摘要翻译： 用于在其类似物上产生超氧化物歧化酶的改进的质粒，其在引入含有不耐热阻遏物CI的合适宿主中时，使宿主能够实现编码超氧化物歧化酶或类似物的基因的表达。 该质粒是双链DNA分子，其以5'至3'的顺序包括以下：来自λ噬菌体的启动子和操纵子PLOL; N利用场所; 第一限制性内切酶位点，其允许置换随后的核糖体结合位点; 核糖体结合位点; ATG起始密码子; 第二限制酶位点; 编码超氧化物歧化酶或类似物的基因; 复制起点和当质粒存在于宿主中时显示的与可选择或可鉴定的表型性状相关的基因。 PLOL的3'端与N利用位点的5'端之间的距离小于约80个碱基对。 N利用位点的3'末端与核糖体结合位点的5'末端之间的距离小于约300个碱基对。 宿主载体系统已经由这些质粒构建并用于产生人超氧化物歧化酶及其类似物。 这种SOD或类似物可用于催化超氧自由基的减少，减少再灌注损伤，延长分离器官的存活时间并减少脊髓损伤。