公开(公告)号：US20240218380A1

公开(公告)日：2024-07-04

申请号：US18582939

申请日：2024-02-21

申请人： Eligo Bioscience ,  Institut Pasteur

发明人： Antoine Decrulle ,  Jesus Fernandez Rodriguez ,  Xavier Duportet ,  David Bikard

IPC分类号： C12N15/73 ,  A61K35/76 ,  A61P31/04 ,  C12N7/00 ,  C12N15/74

CPC分类号： C12N15/73 ,  A61K35/76 ,  A61P31/04 ,  C12N7/00 ,  C12N15/74 ,  C12N2795/10332 ,  C12N2800/10 ,  C12N2800/101

摘要： The present invention relates to a vector, preferably included in a delivery vehicle, comprising no more than 100, preferably no more than 10, restriction sites recognized by the restriction enzymes encoded by each bacterium of a group of bacteria of interest. The invention also relates to the use of said vector, preferably included in a delivery vehicle, as a drug, especially in the treatment of a disease in a patient in need thereof.

公开(公告)号：US11760986B2

公开(公告)日：2023-09-19

申请号：US17123632

申请日：2020-12-16

申请人： President and Fellows of Harvard College

发明人： David R. Liu ,  Bryan Dickinson ,  Michael S. Packer ,  Ahmed Hussein Badran

IPC分类号： C12N15/10 ,  C12N9/50 ,  C12N15/73 ,  C12N7/00 ,  C12N9/12 ,  C12N15/52 ,  C12N15/62 ,  C12N15/86

CPC分类号： C12N9/50 ,  C12N7/00 ,  C12N9/1247 ,  C12N15/1037 ,  C12N15/1058 ,  C12N15/52 ,  C12N15/62 ,  C12N15/73 ,  C12N15/86 ,  C12Y207/07006 ,  C12Y304/00 ,  C07K2319/50 ,  C12N2795/00022

摘要： Some aspects of this disclosure provide methods for phage-assisted continuous evolution (PACE) of proteases. Some aspects of this invention provide methods for evaluating and selecting protease inhibitors based on the likelihood of the emergence of resistant proteases as determined by the protease PACE methods provided herein. Some aspects of this disclosure provide strategies, methods, and reagents for protease PACE, including fusion proteins for translating a desired protease activity into a selective advantage for phage particles encoding a protease exhibiting such an activity and improved mutagenesis-promoting expression constructs. Evolved proteases that recognize target cleavage sites which differ from their canonical cleavage site are also provided herein.

公开(公告)号：US20220213224A1

公开(公告)日：2022-07-07

申请号：US17470778

申请日：2021-09-09

申请人： Hoffmann-La Roche Inc.

发明人： Christian KLEIN ,  Ekkehard MOESSNER ,  Ralf HOSSE ,  Peter BRUENKER ,  Pablo UMANA ,  Christiane NEUMANN

IPC分类号： C07K16/46 ,  C07K16/28 ,  C07K16/30 ,  A61K39/00 ,  A61K39/385 ,  C12N15/73

摘要： The present invention generally relates to novel bispecific antigen binding molecules for T cell activation and re-direction to specific target cells comprising a common light chain. In addition, the present invention relates to polynucleotides encoding such bispecific antigen binding molecules, and vectors and host cells comprising such polynucleotides. The invention further relates to methods for producing the bispecific antigen binding molecules of the invention, and to methods of using these bispecific antigen binding molecules in the treatment of disease.

公开(公告)号：US11046963B2

公开(公告)日：2021-06-29

申请号：US15569024

申请日：2016-04-29

申请人： enGenes Biotech GmbH

发明人： Juergen Mairhofer ,  Gerald Striedner ,  Reingard Grabherr ,  Monika Wilde

IPC分类号： C12N5/00 ,  C12N15/73 ,  C07K14/005 ,  C12N9/12

摘要： The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to methods of increasing the expression level of a protein of interest of a bacterial host cell in a production process. The invention relates particularly to improving the capacity of a bacterial host cell to express a protein of interest by expressing a phage protein during the production process which inhibits growth of the bacterial host cell. Decoupling growth of the bacterial host cell of manufacturing of the protein of interest during the production process reduces (i) the metabolic burden, (ii) oxygen demand, (iii) metabolic heat development, and (iv) avoids stress response caused by heterologous protein expression and thereby increases the capacity of a host cell to produce the protein of interest. The present invention also relates to uses of the host cell for protein expression, cell culture technology, and more specifically to culturing host cells to produce a protein of interest.

公开(公告)号：US20190161779A1

公开(公告)日：2019-05-30

申请号：US16063651

申请日：2016-11-17

申请人： Jiangnan University

发明人： Zhiming RAO ,  Junping ZHOU ,  Taowei YANG ,  Xian ZHANG ,  Meijuan XU ,  CaiZhe ZHANG ,  Yunlong QI ,  Junxian ZHENG

IPC分类号： C12P13/04 ,  C12N15/73

摘要： The present invention discloses a single-cell factory for efficiently synthesizing α-aminobutyric acid and construction and application thereof, which belong to the technical field of microorganisms. The present invention expresses an L-threonine deaminase gene, an L-amino acid dehydrogenase gene and a dehydrogenase gene for providing cofactor NADH cycle in tandem to construct a recombinant Escherichia coli single-cell factory which is used for efficiently synthesizing α-aminobutyric acid. The expression level of the L-threonine deaminase is optimized and controlled by an RBS sequence, so that the problem of transformation inhibition caused by the rapid accumulation of an intermediate product ketobutyric acid is solved, moreover, the expression level of the dehydrogenase for providing cofactor NADH cycle is optimized and controlled by a promoter and an RBS sequence, consequently, the NADH regeneration rate is increased, and ultimately, yield is increased. Utilizing the single-cell factory to carry out whole-cell transformation can reduce obstacles to substances getting in and out, increase the transformation rate and promote the intracellular cycle of cofactors without requiring exogenous addition, and the cost is low. Within 20 h, the yield of the recombinant Escherichia coli single-cell factory in a 5 L fermentation tank is 204 g·L−1, the space-time yield is 10.2 g·L−1·h−1, and a practical effective strategy is provided for industrialized production

公开(公告)号：US20180282737A1

公开(公告)日：2018-10-04

申请号：US15569024

申请日：2016-04-29

申请人： enGenes Biotech GmbH

发明人： Juergen MAIRHOFER

IPC分类号： C12N15/73 ,  C12N9/12 ,  C07K14/005

摘要： The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to methods of increasing the expression level of a protein of interest of a bacterial host cell in a production process. The invention relates particularly to improving the capacity of a bacterial host cell to express a protein of interest by expressing a phage protein during the production process which inhibits growth of the bacterial host cell. Decoupling growth of the bacterial host cell of manufacturing of the protein of interest during the production process reduces (i) the metabolic burden, (ii) oxygen demand, (iii) metabolic heat development, and (iv) avoids stress response caused by heterologous protein expression and thereby increases the capacity of a host cell to produce the protein of interest. The present invention also relates to uses of the host cell for protein expression, cell culture technology, and more specifically to culturing host cells to produce a protein of interest.

公开(公告)号：US09765343B2

公开(公告)日：2017-09-19

申请号：US14681591

申请日：2015-04-08

申请人： Lucigen Corporation

发明人： Ronald Godiska ,  David A. Mead ,  Nikolai V. Ravin

IPC分类号： C12N15/70 ,  C12N15/74 ,  C12N15/73 ,  C12N9/90 ,  C12P19/34

CPC分类号： C12N15/73 ,  C12N9/90 ,  C12N15/70 ,  C12P19/34

摘要： Linear vectors derived from bacteriophage of E. coli and host cells suitable for cloning are provided. The linear vectors include a left arm comprising a left telomere and a first selectable marker, a right arm comprising a right telomere and a second selectable marker and a cloning region located between the left arm and the right arm. Optional further components of the vector include transcriptional termination sequences, multiple cloning sites and reporter stuffer regions.

公开(公告)号：US09469857B2

公开(公告)日：2016-10-18

申请号：US13274637

申请日：2011-10-17

申请人： Michael R. Slater ,  James Robert Hartnett

发明人： Michael R. Slater ,  James Robert Hartnett

IPC分类号： C12N15/73 ,  C12P21/02 ,  C12N15/63 ,  C12N15/10 ,  C12N15/64 ,  C12N15/65 ,  C12N15/66 ,  G06F19/28

CPC分类号： C12N15/73 ,  C12N15/10 ,  C12N15/63 ,  C12N15/64 ,  C12N15/65 ,  C12N15/66 ,  G06F19/28

摘要： The invention provides vectors and methods for directional cloning.

公开(公告)号：US08962292B2

公开(公告)日：2015-02-24

申请号：US13641584

申请日：2011-04-15

申请人： Philippe Jais

发明人： Philippe Jais

IPC分类号： C12N15/73 ,  C12N9/12 ,  C12N9/10 ,  C12N9/14 ,  A61K38/00

CPC分类号： C12P19/34 ,  A61K38/00 ,  C07K2319/00 ,  C12N9/1007 ,  C12N9/1241 ,  C12N9/1247 ,  C12N9/14 ,  C12Y201/01056 ,  C12Y306/04013 ,  Y02A50/411 ,  Y02A50/469

摘要： The invention provides a chimeric enzyme comprising at least one catalytic domain of a RNA triphosphatase, at least one catalytic domain of a guanylyltransferase, at least one catalytic domain of a N7-guanine methyltransferase, and at least one catalytic domain of a DNA-dependant RNA polymerase. The invention also provides pharmaceutical composition comprising said chimeric enzyme and uses of said chimeric enzyme.

摘要翻译： 本发明提供了包含至少一种RNA三磷酸酶的催化​​结构域，鸟苷酰基转移酶的至少一个催化结构域，N7-鸟嘌呤甲基转移酶的至少一个催化结构域和至少一个DNA依赖性RNA的催化结构域的嵌合酶 聚合酶。 本发明还提供了包含所述嵌合酶的药物组合物和所述嵌合酶的用途。

公开(公告)号：US20140147890A1

公开(公告)日：2014-05-29

申请号：US14131320

申请日：2012-07-06

申请人： Cedric Szpirer

发明人： Cedric Szpirer

IPC分类号： C12N15/73

CPC分类号： C12N7/00 ,  C12N15/70 ,  C12N2795/10343 ,  C12N2795/10351 ,  C12N2795/10352 ,  C12P1/04 ,  C12P21/02

摘要： The present invention relates to a genetically modified phage and use thereof in a method for producing a bio molecule of interest.

摘要翻译： 本发明涉及遗传修饰的噬菌体及其在生产感兴趣的生物分子的方法中的用途。