公开(公告)号：US20110118127A1

公开(公告)日：2011-05-19

申请号：US12720134

申请日：2010-03-09

申请人： Jae-Chun RYU ,  Youn Jung KIM ,  Mee SONG ,  Mi-Kyung SONG ,  Han Seam CHOI ,  Ji Hee KIM ,  Jina LEE

发明人： Jae-Chun RYU ,  Youn Jung KIM ,  Mee SONG ,  Mi-Kyung SONG ,  Han Seam CHOI ,  Ji Hee KIM ,  Jina LEE

IPC分类号： C40B30/00 ,  C40B40/08

CPC分类号： C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/142 ,  C12Q2600/158

摘要： The present invention relates to a marker gene for screening a drug inducing toxicity in human and a screening method using the same. More precisely, the invention relates to a microarray on which marker genes up-or down-regulated specifically by 16 drugs inducing pulmonary toxicity, teratogenicity, nephrotoxicity, cardiotoxicity or mutation (Methotrexate, Nitrofurantoin, Amiodarone, Carbamazepine, Valproic acid, Thalidomide, Cisplatin, Gentamycin, Amphotericine, Furylfuramide, N-nitroso-N-methylurea, methylmethanesulfonate, 4-nitroquinoline-N-oxide, 2-nitrofluorene, Doxorubicin and Daunorubicin) are integrated, a kit comprising the said microarray, and a screening method of a drug inducing toxicity in human using the same. The DNA microarray containing the marker gene of the present invention facilitates the construction of Toxtarget Array for screening a drug inducing toxicity in human using drug-specific genes, suggesting that this chip can be effectively used for monitoring drugs or chemicals carrying toxicity to human or determining risks thereof and also it can be used as a tool for examining mechanisms of toxicity/side effects caused by the drugs.

摘要翻译： 本发明涉及用于筛选诱导人体毒性的药物的标记基因和使用其的筛选方法。 更准确地说，本发明涉及一种微阵列，其上由16种药物诱导肺毒性，致畸性，肾毒性，心脏毒性或突变特异性地上调或下调的标记基因（甲氨蝶呤，呋喃妥因，胺碘酮，卡马西平，丙戊酸，沙利度胺，顺铂， 整合了庆大霉素，两性霉素，呋喃酰胺，N-亚硝基-N-甲基脲，甲基磺酸甲酯，4-硝基喹啉-N-氧化物，2-硝基芴，多柔比星和柔红霉素），包含所述微阵列的试剂盒和诱导药物的筛选方法 人体毒性相同。 含有本发明标记基因的DNA微阵列有利于构建Toxtarget Array，用于筛选使用药物特异性基因在人体中诱导毒性的药物，表明该芯片可有效用于监测对人体具有毒性或确定性的药物或化学物质 也可以作为检测药物毒性/副作用机制的工具。