公开(公告)号：US08053235B2

公开(公告)日：2011-11-08

申请号：US11261429

申请日：2005-10-28

申请人： Jane H. Buckner ,  Mindi R. Walker

发明人： Jane H. Buckner ,  Mindi R. Walker

IPC分类号： A01N1/02 ,  C12N5/071

CPC分类号： C12N5/0636 ,  A61K39/0008 ,  A61K39/001 ,  A61K2035/122 ,  A61K2039/5158 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2502/11

摘要： The present invention provides methods for generating mammalian T cell populations comprising antigen-specific CD4+CD25+ regulatory T cells from freshly isolated CD4+CD25− T cells. The method comprises selecting CD4+CD25− T cells from a sample obtained from a mammalian subject; determining the MHC Class II type of the subject; inducing the generation of antigen-specific regulatory T cells by contacting the isolated CD4+CD25− T cells in a culture vessel with an induction agent for a time period sufficient to generate antigen-specific CD4+CD25+ regulatory T cells; and selecting the CD4+CD25+ antigen-specific regulatory T cells by sorting the cells in the induction culture with a selection agent comprising at least one artificial multimeric MHC Class II/peptide complex that corresponds to the MHC Class II type of the subject.

摘要翻译： 本发明提供了用于从新分离的CD4 + CD25-T细胞产生包含抗原特异性CD4 + CD25 +调节性T细胞的哺乳动物T细胞群的方法。 该方法包括从哺乳动物受试者获得的样品中选择CD4 + CD25-T细胞; 确定受试者的MHC II类型; 通过使培养容器中的分离的CD4 + CD25-T细胞与诱导剂接触足以产生抗原特异性CD4 + CD25 +调节性T细胞的时间段来诱导产生抗原特异性调节性T细胞; 以及通过用包含至少一种对应于受试者的MHC II类类型的人造多聚体MHC II类/肽复合物的选择剂分选诱导培养物中的细胞来选择CD4 + CD25 +抗原特异性调节性T细胞。