公开(公告)号：US20190263915A1

公开(公告)日：2019-08-29

申请号：US16227597

申请日：2018-12-20

Applicant: Janssen Biotech, Inc.

Inventor： Shalom GOLDBERG ,  Steven JACOBS ,  Tricia LIN ,  Karyn O'Neil

IPC: C07K16/28 ,  G01N33/74 ,  C07K14/78

Abstract: Cysteine engineered monospecific and bispecific EGFR and/or c-Met FN3 domain containing molecules comprising one or more free cysteine amino acids are prepared by mutagenizing a nucleic acid sequence of a parent molecule and replacing one or more amino acid residues by cysteine to encode the cysteine engineered FN3 domain containing monospecific or bispecific molecules; expressing the cysteine engineered FN3 domain containing molecules; and recovering the cysteine engineered FN3 domain containing molecule. Isolated cysteine engineered monospecific or bispecific FN3 domain containing molecules may be covalently attached to a detection label or a drug moiety and used therapeutically.