公开(公告)号：US20040268432A1

公开(公告)日：2004-12-30

申请号：US10625821

申请日：2003-07-22

Applicant: Japan Science and Technology Corporation

Inventor： Satoshi Mori ,  Hiromi Nakanishi ,  Hiroyuki Oki ,  Hirotaka Yamaguchi

IPC: C07H021/04 ,  C12N015/82 ,  C12N015/87

CPC classification number: C12N9/0036 ,  C12N15/8216 ,  C12N15/8243 ,  C12N15/8261 ,  Y02A40/146

Abstract: A method for achieving the sufficient expression of a gene in a useful higher plant which has been transformed by transferring the above gene encoding a protein having a function carried by another organism so as to impart the function to the plant. Namely, a method for transforming a useful plant by transferring a gene of another species into the plant characterized in that the region of a factor relating to the poly(A) addition of the mRNA of the useful plant to be transformed contained in the base sequence of the gene of the other species is denatured into another base sequence not relating to the poly(A) addition of the mRNA without substantially altering the function of the protein encoded by the gene to be transferred; and a gene usable in the gene transfer.

Abstract translation: 通过转移上述编码具有由另一种生物体携带的功能的蛋白质的基因转化的有用的较高植物中的基因的充分表达以赋予植物功能的方法。 即，通过将其他物种的基因转移到植物中来转化有用植物的方法，其特征在于与碱基序列中包含的待转化有用植物的mRNA的poly（A）加成相关的因子的区域 的其他物种的基因被变性成不与mRNA的poly（A）添加无关的另一个碱基序列，而基本上不改变由待转移的基因编码的蛋白质的功能; 和可用于基因转移的基因。