公开(公告)号：US20060194259A1

公开(公告)日：2006-08-31

申请号：US10518298

申请日：2003-06-16

申请人： Jean-Luc Galzi ,  Marcel Hibert ,  Jean-Jacques Bourguignon ,  Emalina Maillet

发明人： Jean-Luc Galzi ,  Marcel Hibert ,  Jean-Jacques Bourguignon ,  Emalina Maillet

IPC分类号： G01N33/567 ,  G01N33/53

CPC分类号： G01N33/566 ,  C07K5/06086 ,  G01N33/542 ,  G01N33/557

摘要： A method for isolating an allosteric effector of a receptor, by determining the variation of the dissociation kinetics of the complex formed between the receptor and one of its ligands in the presence of the allosteric effector, as compared to the kinetics dissociation, in the absence of the effector, and/or the amplitude of the linkage formed between the receptor and the ligand in the presence of the allosteric effector, as compared to the amplitude in the absence of the effector. The receptor and ligand are being involved in at least one biological response, and the allosteric effector is capable of modulating at least one of the responses. The receptor is marked by at least one fluorescent protein, and the ligand by a molecule capable of absorbing light, or by a fluorescent substance.

摘要翻译： 通过在不存在的情况下通过确定在变构效应物存在下与受体与其配体之间形成的络合物的解离动力学的变化，与动力学解离相比，分离受体的变构效应物的方法 与在不存在效应物时的振幅相比，在变构效应器存在下，在受体和配体之间形成的连接的效应物和/或振幅。 受体和配体参与至少一种生物反应，并且变构效应子能够调节至少一种应答。 该受体由至少一种荧光蛋白标记，该配体由能够吸收光的分子或荧光物质标记。