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公开(公告)号：US20050158711A1
公开(公告)日：2005-07-21
申请号：US10987388
申请日：2004-11-12
IPC分类号： A61K38/17 , C07H21/04 , C07K20060101 , C07K14/705 , C12N7/00 , C12N15/86 , C12Q1/68 , C12Q1/70
CPC分类号： C07H21/04
摘要： The invention provides for polynucleotides and vectors comprising at least two tag sequences. In particular, preferred vectors are viral vectors. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for methods of using the polynucleotides and vectors of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.
摘要翻译： 本发明提供了包含至少两个标签序列的多核苷酸和载体。 特别地，优选的载体是病毒载体。 本发明还提供了包含链霉亲和素结合肽序列和钙调蛋白结合肽序列的多核苷酸和载体。 本发明还提供多核苷酸和载体，其中目的基因与至少两个标签序列融合，例如链霉抗生物素蛋白结合肽序列和钙调蛋白结合肽序列。 本发明还提供了使用本发明的多核苷酸和载体用于检测和/或分离蛋白质复合物或鉴定感兴趣的蛋白质的结合配偶体的方法。
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公开(公告)号：US20050106663A1
公开(公告)日：2005-05-19
申请号：US10712137
申请日：2003-11-13
IPC分类号： A61K38/17 , C07H21/04 , C07K20060101 , C07K14/705 , C12N7/00 , C12N15/86 , C12Q1/68 , C12Q1/70
CPC分类号： C07H21/04
摘要： The invention provides for polynucleotides and vectors comprising at least two tag sequences. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for the chimeric proteins encoded by these polynucleotides. The invention also provides for methods of using the polynucleotides of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.
摘要翻译： 本发明提供了包含至少两个标签序列的多核苷酸和载体。 本发明还提供了包含链霉亲和素结合肽序列和钙调蛋白结合肽序列的多核苷酸和载体。 本发明还提供多核苷酸和载体，其中目的基因与至少两个标签序列融合，例如链霉亲和素结合肽序列和钙调蛋白结合肽序列。 本发明还提供了由这些多核苷酸编码的嵌合蛋白质。 本发明还提供了使用本发明的多核苷酸检测和/或分离蛋白质复合物或鉴定感兴趣的蛋白质的结合配偶体的方法。
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公开(公告)号：US20080213760A1
公开(公告)日：2008-09-04
申请号：US11773353
申请日：2007-07-03
申请人： Jeffrey C. Braman , Carsten-Peter Carstens , Natalia Novoradovskaya , Rajesh Bagga , Lee Scott Basehore
发明人： Jeffrey C. Braman , Carsten-Peter Carstens , Natalia Novoradovskaya , Rajesh Bagga , Lee Scott Basehore
IPC分类号： C12Q1/68
CPC分类号： C07H21/04
摘要： The invention provides for polynucleotides and vectors comprising at least two tag sequences. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for the chimeric proteins encoded by these polynucleotides. The invention also provides for methods of using the polynucleotides of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.
摘要翻译： 本发明提供了包含至少两个标签序列的多核苷酸和载体。 本发明还提供了包含链霉亲和素结合肽序列和钙调蛋白结合肽序列的多核苷酸和载体。 本发明还提供多核苷酸和载体，其中目的基因与至少两个标签序列融合，例如链霉抗生物素蛋白结合肽序列和钙调蛋白结合肽序列。 本发明还提供了由这些多核苷酸编码的嵌合蛋白质。 本发明还提供了使用本发明的多核苷酸检测和/或分离蛋白质复合物或鉴定感兴趣的蛋白质的结合配偶体的方法。
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公开(公告)号：US07238478B2
公开(公告)日：2007-07-03
申请号：US10712137
申请日：2003-11-13
申请人： Jeffrey C. Braman , Carsten-Peter Carstens , Natalia Novoradovskaya , Rajesh Bagga , Lee Scott Basehore
发明人： Jeffrey C. Braman , Carsten-Peter Carstens , Natalia Novoradovskaya , Rajesh Bagga , Lee Scott Basehore
CPC分类号： C07H21/04
摘要： The invention provides for polynucleotides and vectors comprising at least two tag sequences. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for the chimeric proteins encoded by these polynucleotides. The invention also provides for methods of using the polynucleotides of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.
摘要翻译： 本发明提供了包含至少两个标签序列的多核苷酸和载体。 本发明还提供了包含链霉亲和素结合肽序列和钙调蛋白结合肽序列的多核苷酸和载体。 本发明还提供多核苷酸和载体，其中目的基因与至少两个标签序列融合，例如链霉抗生物素蛋白结合肽序列和钙调蛋白结合肽序列。 本发明还提供了由这些多核苷酸编码的嵌合蛋白质。 本发明还提供了使用本发明的多核苷酸检测和/或分离蛋白质复合物或鉴定感兴趣的蛋白质的结合配偶体的方法。
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公开(公告)号：US07235362B2
公开(公告)日：2007-06-26
申请号：US10987388
申请日：2004-11-12
申请人： Jeffrey Carl Braman , Carsten-Peter Carstens , Natalia Novoradovskaya , Rajesh Bagga , Lee Scott Basehore
发明人： Jeffrey Carl Braman , Carsten-Peter Carstens , Natalia Novoradovskaya , Rajesh Bagga , Lee Scott Basehore
CPC分类号： C07H21/04
摘要： The invention provides for polynucleotides and vectors comprising at least two tag sequences. In particular, preferred vectors are viral vectors. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for methods of using the polynucleotides and vectors of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.
摘要翻译： 本发明提供了包含至少两个标签序列的多核苷酸和载体。 特别地，优选的载体是病毒载体。 本发明还提供了包含链霉亲和素结合肽序列和钙调蛋白结合肽序列的多核苷酸和载体。 本发明还提供多核苷酸和载体，其中目的基因与至少两个标签序列融合，例如链霉抗生物素蛋白结合肽序列和钙调蛋白结合肽序列。 本发明还提供了使用本发明的多核苷酸和载体用于检测和/或分离蛋白质复合物或鉴定感兴趣的蛋白质的结合配偶体的方法。
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