-
公开(公告)号：US07901670B2
公开(公告)日：2011-03-08
申请号：US10581597
申请日：2004-12-04
申请人： Ji Sook Park , Jong Sang Chung , Min Ji Baek , Jee Won Ahn , Ki Wan Kim , Hyung Ki Park , Dong Eok Lee , Myung Suk Oh
发明人： Ji Sook Park , Jong Sang Chung , Min Ji Baek , Jee Won Ahn , Ki Wan Kim , Hyung Ki Park , Dong Eok Lee , Myung Suk Oh
CPC分类号： C07K14/565 , C12P21/02
摘要： Provided is a process for purifying human interferon beta from a recombinant human interferon beta-containing culture comprising performing affinity chromatography and cation exchange chromatography, wherein the affinity chromatography includes: adsorbing the interferon beta-containing culture to an equilibrated affinity chromatography column, followed by washing with an equilibration buffer solution; washing the column with a washing buffer solution A of pH 6.5-7.5 containing 30-60 wt % of propylene glycol and a washing buffer solution B of pH 6.5-7.5 containing 10-30 wt % of propylene glycol and 1-2M NaCl; and eluting a human interferon beta-containing fraction with a buffer solution of pH 6.5-7.5 containing 40-60 wt % of propylene glycol and 1-2M NaCl.
摘要翻译： 提供了一种从重组人类干扰素β的培养物中纯化人类干扰素β的方法，包括进行亲和层析和阳离子交换层析，其中亲和层析包括：将含干扰素β的培养物吸附到平衡的亲和色谱柱上，然后洗涤 用平衡缓冲溶液; 用含有30-60重量％丙二醇的pH 6.5-7.5的洗涤缓冲溶液A和pH6.5-7.5的洗涤缓冲液B洗涤柱子，其中含有10-30重量％的丙二醇和1-2M的NaCl; 并用含有40-60重量％丙二醇和1-2M NaCl的pH 6.5-7.5的缓冲溶液洗脱人类含有干扰素β的级分。
-
公开(公告)号：US07780960B2
公开(公告)日：2010-08-24
申请号：US10581602
申请日：2004-12-04
申请人： Ji Sook Park , Jong Sang Chung , Min Ji Baek , Jee Won Ahn , Ki Wan Kim , Hyung Ki Park , Dong Eok Lee , Myung Suk Oh
发明人： Ji Sook Park , Jong Sang Chung , Min Ji Baek , Jee Won Ahn , Ki Wan Kim , Hyung Ki Park , Dong Eok Lee , Myung Suk Oh
CPC分类号： C07K14/565 , C07K5/06139
摘要： Provided is a process for purifying human interferon beta from a recombinant human interferon beta-containing culture comprising performing affinity chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), wherein the affinity chromatography includes: adsorbing the interferon beta-containing culture to an equilibrated affinity chromatography column, followed by washing with an equilibration buffer solution; washing the column with a washing buffer solution A of pH 6.5-7.5 containing 30-60 wt % of propylene glycol and a washing buffer solution B of pH 6.5-7.5 containing 10-30 wt % of propylene glycol and 1-2M NaCl; and eluting a human interferon beta-containing fraction with a buffer solution of pH 6.5-7.5 containing 40-60 wt % of propylene glycol and 1-2M NaCl.
摘要翻译： 提供了一种从重组人类干扰素β培养物中纯化人类干扰素β的方法，包括进行亲和层析和反相高效液相色谱（RP-HPLC），其中亲和层析包括：吸附含有干扰素β的培养物 平衡的亲和层析柱，然后用平衡缓冲液洗涤; 用含有30-60重量％丙二醇的pH 6.5-7.5的洗涤缓冲溶液A和pH6.5-7.5的洗涤缓冲液B洗涤柱子，其中含有10-30重量％的丙二醇和1-2M的NaCl; 并用含有40-60重量％丙二醇和1-2M NaCl的pH 6.5-7.5的缓冲溶液洗脱人类含有干扰素β的级分。
-
公开(公告)号：US07311903B2
公开(公告)日：2007-12-25
申请号：US10653350
申请日：2003-09-02
申请人： Eun Jung Lee , Hyung Ki Park , Hyun Seok Kim , Ji Sook Park , Yeon Hyang Kim , Hyune Soo Lee , Hyung Kon Koh , Myung Suk Oh
发明人： Eun Jung Lee , Hyung Ki Park , Hyun Seok Kim , Ji Sook Park , Yeon Hyang Kim , Hyune Soo Lee , Hyung Kon Koh , Myung Suk Oh
CPC分类号： C07K14/56
摘要： The present invention relates to an amino acid-modified human interferon alpha isoform having at least one of the Asn-Xaa-Ser/Thr (N-X-S/T) sequence formed at a specific site so that glycosylation takes place at this site and a gene encoding the same, an expression vector comprising the gene, and a method for producing glycosylated human interferon alpha isoform by transforming or transfecting an eukaryotic cell with the expression vector, culturing the transfected or transformed cell and isolating the glycosylated human interferon alpha isoform from the culture, the glycosylated human interferon alpha isoform produced therefrom and a pharmaceutical composition comprising the same.
摘要翻译： 本发明涉及具有在特定部位形成的至少一种Asn-Xaa-Ser / Thr（NXS / T）序列的氨基酸修饰的人类干扰素α同种型，使得在该位点发生糖基化，并编码基因 相同的包含该基因的表达载体和通过用表达载体转化或转染真核细胞来产生糖基化的人干扰素α同种型的方法，培养转染或转化的细胞并从培养物中分离糖基化的人干扰素α同种型， 由其制备的糖基化的人干扰素α同种型和包含其的药物组合物。
-
-
搜索结果
3 条记录 (耗时 0.033 秒)
