公开(公告)号：US20220204953A1

公开(公告)日：2022-06-30

申请号：US17694779

申请日：2022-03-15

申请人： Jiangnan University

发明人： Liming Liu ,  Xin Xu ,  Wei Song ,  Xiulai Chen ,  Jia Liu ,  Cong Gao ,  Jing Wu ,  Liang Guo

IPC分类号： C12N9/16 ,  C12N15/70 ,  C12P17/04

摘要： Disclosed is a mutant of acid phosphatase and an application thereof, belonging to the technical field of biological engineering. The disclosure provides a mutant of acid phosphatase PaAPaseMu3. By expressing the mutant of acid phosphatase PaAPaseMu3 in Escherichia coli and using a whole-cell conversion method, L-ascorbic acid is transformed into L-ascorbate-2-phosphate. Moreover, a catalytic system of the mutant of acid phosphatase PaAPaseMu3 is optimized from the aspects of reaction pH and temperature, so that a yield of L-ascorbate-2-phosphate can reach 90.1 g/L and a molar yield can reach 75.1%. Therefore, the problems of a high substrate cost, environmental pollution and the like before are greatly reduced, laying a foundation for the industrial green production of L-ascorbate-2-phosphate.