公开(公告)号：US20220204953A1

公开(公告)日：2022-06-30

申请号：US17694779

申请日：2022-03-15

申请人： Jiangnan University

发明人： Liming Liu ,  Xin Xu ,  Wei Song ,  Xiulai Chen ,  Jia Liu ,  Cong Gao ,  Jing Wu ,  Liang Guo

IPC分类号： C12N9/16 ,  C12N15/70 ,  C12P17/04

摘要： Disclosed is a mutant of acid phosphatase and an application thereof, belonging to the technical field of biological engineering. The disclosure provides a mutant of acid phosphatase PaAPaseMu3. By expressing the mutant of acid phosphatase PaAPaseMu3 in Escherichia coli and using a whole-cell conversion method, L-ascorbic acid is transformed into L-ascorbate-2-phosphate. Moreover, a catalytic system of the mutant of acid phosphatase PaAPaseMu3 is optimized from the aspects of reaction pH and temperature, so that a yield of L-ascorbate-2-phosphate can reach 90.1 g/L and a molar yield can reach 75.1%. Therefore, the problems of a high substrate cost, environmental pollution and the like before are greatly reduced, laying a foundation for the industrial green production of L-ascorbate-2-phosphate.

公开(公告)号：US10308919B2

公开(公告)日：2019-06-04

申请号：US15386588

申请日：2016-12-21

申请人： Jiangnan University

发明人： Liming Liu ,  Wei Song ,  Jing Wu ,  Kai Zheng ,  Xiulai Chen

IPC分类号： C12P17/04 ,  C12N9/16 ,  C12N15/70

摘要： The present invention provides a recombinant strain, construction method thereof and a method for producing acid phosphatase using the recombinant strain. In the invention, the phosphatase gene is obtained from Pseudomonas aeruginosa by a molecular biology method, the constructed expression plasmid is transformed into E. coli BL21 (DE3). The purified enzyme and whole cells were used for the conversion of ascorbic acid to ascorbic acid-2-phosphate. Ascorbic acid-2-phosphate can be efficiently produced by controlling the ratio of substrates. When the conversion reaction is performed at pH 4.5 under 40° C. for 8 h, the output of ascorbic acid-2-phosphate reaches 54.8 g/L, the conversion is 42.9% and the space time yield is 6.9 g/L/h.