公开(公告)号：US10308919B2

公开(公告)日：2019-06-04

申请号：US15386588

申请日：2016-12-21

申请人： Jiangnan University

发明人： Liming Liu ,  Wei Song ,  Jing Wu ,  Kai Zheng ,  Xiulai Chen

IPC分类号： C12P17/04 ,  C12N9/16 ,  C12N15/70

摘要： The present invention provides a recombinant strain, construction method thereof and a method for producing acid phosphatase using the recombinant strain. In the invention, the phosphatase gene is obtained from Pseudomonas aeruginosa by a molecular biology method, the constructed expression plasmid is transformed into E. coli BL21 (DE3). The purified enzyme and whole cells were used for the conversion of ascorbic acid to ascorbic acid-2-phosphate. Ascorbic acid-2-phosphate can be efficiently produced by controlling the ratio of substrates. When the conversion reaction is performed at pH 4.5 under 40° C. for 8 h, the output of ascorbic acid-2-phosphate reaches 54.8 g/L, the conversion is 42.9% and the space time yield is 6.9 g/L/h.