公开(公告)号：US20240060062A1

公开(公告)日：2024-02-22

申请号：US18489954

申请日：2023-10-19

Applicant: Jiangnan University

Inventor： Jie LONG ,  Ziying YE ,  Zhengyu JIN ,  Cheng Lu ,  Xingfei Li ,  Yaoqi TIAN ,  Yuxiang BAI ,  Xing ZHOU ,  Chao QIU ,  Long CHEN ,  Zhengjun XIE

IPC: C12N9/38 ,  C12N15/63

CPC classification number: C12N9/2468 ,  C12N15/63 ,  C12Y302/01081

Abstract: Disclosed are an agarase mutant with improved thermal stability and application thereof, belonging to the fields of genetic engineering technology and enzyme engineering. The present disclosure provides an agarase mutant, which is obtained by mutating the amino acid at the 86th site, the 373rd site, the 374th site, the 496th site, the 507th site, or the 747th site of agarase with an amino acid sequence as shown in SEQ ID NO.1. The agarase mutant provided by the present disclosure improves the thermal stability and the hydrolytic activity of the agarase. Compared with the wild type enzyme, the mutant enzyme shows excellent heat resistance and can be industrially used at a relatively high temperature, so that the utilization rate of agar raw materials and the yield of oligosaccharides from agar are improved, and the mutant enzyme has a good industrial application prospect.

公开(公告)号：US20210371302A1

公开(公告)日：2021-12-02

申请号：US17402871

申请日：2021-08-16

Applicant: Jiangnan University

Inventor： Jinpeng WANG ,  Jing LI ,  Zhengyu JIN ,  Xueming XU ,  Yaoqi TIAN ,  Jie LONG ,  Xing ZHOU ,  Zhengjun XIE

IPC: C02F1/28 ,  B01J20/24 ,  B01J20/28 ,  B01J20/30

Abstract: The disclosure provides a modified cyclodextrin/mesoporous silica for adsorbing Pb and Cd and application thereof, belonging to the technical field of adsorbent materials. By using surface modification, chloroacetic acid is used as anions, and a modified cyclodextrin is grafted onto a surface of a cyclodextrin/mesoporous silica by nucleophilic substitution to prepare the modified cyclodextrin/mesoporous silica adsorbent material. The modified cyclodextrin/mesoporous silica adsorbent material prepared in the disclosure has the advantages of simple preparation method, strong adsorbability, easy separation, good biocompatibility and the like. When the material is used as an adsorbent to adsorb heavy metal ions Pb2+ and Cd2+, maximum removal rates can reach 97.8% and 81.29% respectively. Therefore, the material has wide application prospects in removal of heavy metals in sewage and the like, thereby improving the water environment that people depend on for survival.

公开(公告)号：US20190194708A1

公开(公告)日：2019-06-27

申请号：US16288765

申请日：2019-02-28

Applicant: Jiangnan University

Inventor： Yuxiang BAI ,  Zhengyu JIN ,  Liuxi XIA ,  Yun WU ,  Xueming XU ,  Zhengjun XIE ,  Jinpeng WANG ,  Xing ZHOU

IPC: C12P19/04 ,  C12P19/18

CPC classification number: C12P19/04 ,  C12P19/18 ,  C12P19/20

Abstract: The disclosure herein relates to a method for preparing branched cyclodextrin and application thereof, and belongs to the technical field of synthesis of branched cyclodextrin. The method comprises the following steps: (1) dissolving maltodextrin in a phosphate buffer solution, and adding CGTase for reacting; (2) reducing enzyme activity by a physical method; and (3) adding a saccharifying enzyme to the reaction system of step (2), and performing high-temperature enzyme deactivation to obtain the branched cyclodextrin. The method has mild reaction conditions, and at the same time, cyclodextrin and unreacted substrates can be hydrolyzed via the weak coupling activity of CGTase, thereby realizing effective separation of the branched cyclodextrin.

公开(公告)号：US20240174770A1

公开(公告)日：2024-05-30

申请号：US18525796

申请日：2023-11-30

Applicant: Jiangnan University

Inventor： Xing ZHOU ,  Xiaoli LIANG ,  Zhengyu JIN ,  Ren WANG ,  Zhengjun XIE ,  Jianwei ZHAO ,  Jie LONG ,  Long CHEN ,  Chao QIU

IPC: C08B30/12 ,  A23L29/30

CPC classification number: C08B30/12 ,  A23L29/30

Abstract: A method for preparing V-type porous starch includes: mixing starch and a first ethanol solution in a reaction kettle, adding concentrated hydrochloric acid to the reaction kettle for acidity regulation, and controlling a temperature of a reaction system in the reaction kettle at 80-150° C.; cooling the reaction kettle, and adding a sodium hydroxide solution to the reaction system in the reaction kettle, until a pH of a mixture in the reaction kettle is neutral, to yield a starch solution; and centrifuging the starch solution, washing a collected precipitate with a second ethanol solution, leaching, drying, cooling, and pulverizing, to yield V-type porous starch.

公开(公告)号：US20200000131A1

公开(公告)日：2020-01-02

申请号：US16542740

申请日：2019-08-16

Applicant: Jiangnan University

Inventor： Yaoqi TIAN ,  Canxin CAI ,  Jinling ZHAN ,  Zhengjun XIE ,  Zhengyu JIN ,  Lizhong QIU

IPC: A23L7/104 ,  A23P10/25 ,  C08B31/04

Abstract: The present disclosure discloses a preparation method of low-glycemic index slowly digestible starch-based reconstituted instant rice, and belongs to the technical field of food processing. The preparation method is based on high-temperature fluidization technology to carry out non-crystallization treatment on a solid phase broken rice raw material in a short period of time, and then the material is subjected to a one-step reactive extrusion technology combining enzymatic hydrolysis and esterification treatment to prepare the reconstituted instant rice with high content of slowly digestible starch and low glycemic index. The reconstituted instant rice prepared by the one-step molding extrusion technology is suitable for type II diabetes patients to eat, and at the same time increases the added value of agricultural and sideline products, reduces production cost, and improves production efficiency.