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31 条记录 (耗时 0.023 秒)

    Agarase mutant with improved thermal stability and application thereof
    1.
    发明授权
    Agarase mutant with improved thermal stability and application thereof 有权

    公开(公告)号：US11999981B2

    公开(公告)日：2024-06-04

    申请号：US18489954

    申请日：2023-10-19

    申请人： Jiangnan University

    发明人： Jie Long ,  Ziying Ye ,  Zhengyu Jin ,  Cheng Lu ,  Xingfei Li ,  Yaoqi Tian ,  Yuxiang Bai ,  Xing Zhou ,  Chao Qiu ,  Long Chen ,  Zhengjun Xie

    IPC分类号： C12N9/38 ,  C12N15/63

    CPC分类号： C12N9/2468 ,  C12N15/63 ,  C12Y302/01081

    摘要： Disclosed are an agarase mutant with improved thermal stability and application thereof, belonging to the fields of genetic engineering technology and enzyme engineering. The present disclosure provides an agarase mutant, which is obtained by mutating the amino acid at the 86th site, the 373rd site, the 374th site, the 496th site, the 507th site, or the 747th site of agarase with an amino acid sequence as shown in SEQ ID NO. 1. The agarase mutant provided by the present disclosure improves the thermal stability and the hydrolytic activity of the agarase. Compared with the wild type enzyme, the mutant enzyme shows excellent heat resistance and can be industrially used at a relatively high temperature, so that the utilization rate of agar raw materials and the yield of oligosaccharides from agar are improved, and the mutant enzyme has a good industrial application prospect.

    Agarase and gene thereof
    3.
    发明授权
    Agarase and gene thereof 失效
    琼脂糖酶及其基因

    公开(公告)号：US07622291B2

    公开(公告)日：2009-11-24

    申请号：US11948650

    申请日：2007-11-30

    申请人： Jun Tomono ,  Hiroaki Sagawa ,  Ikunoshin Kato

    发明人： Jun Tomono ,  Hiroaki Sagawa ,  Ikunoshin Kato

    IPC分类号： C07H21/04 ,  C12N1/21 ,  C12N15/63 ,  C12P19/26 ,  C12N9/24 ,  C12N9/40

    CPC分类号： C12Y302/01081 ,  C12N9/2468

    摘要： Production of efficiently producing agaro-oligosaccharide; a polypeptide having an agarase activity which is usable in, for example, efficiently extracting a substance such as a nucleic acid from an agarose gel; the amino acid sequence of the polypeptide; a gene encoding the polypeptide; a process for producing the polypeptide; a process for producing agaro-oligosaccharide; and a process for extracting a substance such as a nucleic acid from an agarose gel.

    摘要翻译： 高效生产琼脂寡糖的生产; 具有琼脂糖酶活性的多肽，其可用于例如从琼脂糖凝胶中有效地提取诸如核酸的物质; 多肽的氨基酸序列; 编码该多肽的基因; 一种生产该多肽的方法; 制备琼脂寡糖的方法; 以及从琼脂糖凝胶中提取核酸等物质的方法。

    AGARASE AND GENE THEREOF
    7.
    发明申请
    AGARASE AND GENE THEREOF 失效
    其基因及其基因

    公开(公告)号：US20080096249A1

    公开(公告)日：2008-04-24

    申请号：US11948650

    申请日：2007-11-30

    申请人： Jun TOMONO ,  Hiroaki Sagawa ,  Ikunoshin Kato

    发明人： Jun TOMONO ,  Hiroaki Sagawa ,  Ikunoshin Kato

    IPC分类号： C12N9/00 ,  C12N15/00 ,  C12N15/11 ,  C12P21/04 ,  C12N5/06

    CPC分类号： C12Y302/01081 ,  C12N9/2468

    摘要： Production of efficiently producing agaro-oligosaccharide; a polypeptide having an agarase activity which is usable in, for example, efficiently extracting a substance such as a nucleic acid from an agarose gel; the amino acid sequence of the polypeptide; a gene encoding the polypeptide; a process for producing the polypeptide; a process for producing agaro-oligosaccharide; and a process for extracting a substance such as a nucleic acid from an agarose gel.

    摘要翻译： 高效生产琼脂寡糖的生产; 具有琼脂糖酶活性的多肽，其可用于例如从琼脂糖凝胶中有效地提取诸如核酸的物质; 多肽的氨基酸序列; 编码该多肽的基因; 一种生产该多肽的方法; 制备琼脂寡糖的方法; 以及从琼脂糖凝胶中提取核酸等物质的方法。

    Agarase and gene thereof
    8.
    发明申请
    Agarase and gene thereof 失效
    琼脂糖酶及其基因

    公开(公告)号：US20040132036A1

    公开(公告)日：2004-07-08

    申请号：US10467824

    申请日：2003-08-13

    发明人： Jun Tomono ,  Hiroaki Sagawa ,  Ikunoshin Kato

    IPC分类号： C12Q001/68 ,  C07H021/04 ,  C12N009/24

    CPC分类号： C12Y302/01081 ,  C12N9/2468

    摘要： Production of efficiently producing agaro-oligosaccharide; a polypeptide having an agarase activity which is usable in, for example, efficiently extracting a substance such as a nucleic acid from an agarose gel; the amino acid sequence of the polypeptide; a gene encoding the polypeptide; a process for producing the polypeptide; a process for producing agaro-oligosaccharide; and a process for extracting a substance such as a nucleic acid from an agarose gel.

    摘要翻译： 高效生产琼脂寡糖的生产; 具有琼脂糖酶活性的多肽，其可用于例如从琼脂糖凝胶中有效地提取诸如核酸的物质; 多肽的氨基酸序列; 编码该多肽的基因; 一种生产该多肽的方法; 制备琼脂寡糖的方法; 以及从琼脂糖凝胶中提取核酸等物质的方法。

    Preparation and use of biofilm-degrading, multiple-specificity, hydrolytic enzyme mixtures
    9.
    发明授权
    Preparation and use of biofilm-degrading, multiple-specificity, hydrolytic enzyme mixtures 有权
    生物膜降解，多特异性，水解酶混合物的制备和使用

    公开(公告)号：US06759040B1

    公开(公告)日：2004-07-06

    申请号：US09508431

    申请日：2000-08-10

    申请人： David M. Manyak ,  Ronald M. Weiner ,  Peter S. Carlson ,  Ernesto J. Quintero

    发明人： David M. Manyak ,  Ronald M. Weiner ,  Peter S. Carlson ,  Ernesto J. Quintero

    IPC分类号： A61K3846

    CPC分类号： A61L2/186 ,  A61K38/00 ,  A61L12/082 ,  A61L2202/24 ,  C11D3/0078 ,  C11D3/38636 ,  C11D11/0041 ,  C12N9/14 ,  C12N9/2442 ,  C12N9/2468 ,  C12Y302/01014 ,  C12Y302/01081

    摘要： The present invention is directed to the production and use of custom tailored, bacterial enzyme mixtures or components thereof for degrading biofilms in both industrial and therapeutic applications. The industrial applications include but are not limited to the use biofilm-degrading, multiple specificity, hydrolytic enzyme mixtures for removing or preventing the formation of biofilms in water cooling towers, industrial process piping, heat exchangers, in food processing or food preparation, in potable water systems, reservoirs, swimming pools, or related sanitary water systems, and on membranes such as those used for desalinization, industrial processes, or related applications. The therapeutic applications include but are not limited to the use of therapeutically-useful, multiple-specificity, hydrolytic enzyme mixtures for the prevention or treatment of dental caries, periodontal disease, cystic fibrosis or the complications or symptoms of cystic fibrosis, removal of biofilms from contact lenses, and diseases or complications associated with biofilm formation on implantable medical devices such as cardiovascular devices.

    摘要翻译： 本发明涉及定制的细菌酶混合物或其组分在工业和治疗应用中降解生物膜的生产和使用。 工业应用包括但不限于使用生物膜降解，多重特异性，水解酶混合物，用于去除或防止水冷却塔，工业过程管道，热交换器，食品加工或食品制备中的生物膜的形成，在饮用水 水系统，水库，游泳池或相关的卫生水系统，以及诸如用于脱盐，工业过程或相关应用的膜。 治疗应用包括但不限于使用治疗有用的多特异性水解酶混合物来预防或治疗龋齿，牙周病，囊性纤维化或囊性纤维化的并发症或症状，从生物膜中去除生物膜 隐形眼镜，以及与可植入医疗装置如心血管装置上的生物膜形成相关的疾病或并发症。