公开(公告)号：US06790937B2

公开(公告)日：2004-09-14

申请号：US10083424

申请日：2002-02-26

申请人： Jin-Kyoo Kim ,  Jae-Yong Han ,  Ki-Duck Song ,  Sung-Won Kim ,  Won-Gi Min ,  Eun-Jung Son ,  Hyun Soon Lillehoj ,  Erik Peter Lillehoj

发明人： Jin-Kyoo Kim ,  Jae-Yong Han ,  Ki-Duck Song ,  Sung-Won Kim ,  Won-Gi Min ,  Eun-Jung Son ,  Hyun Soon Lillehoj ,  Erik Peter Lillehoj

IPC分类号： C12P2108

CPC分类号： C07K16/20 ,  C07K2317/622 ,  Y10S530/822

摘要： The present invention relates to (a) variable regions of heavy and light chains of an antibody specific to a surface antigen in sporozoite of Eimeria spp.; (b) a recombinant scFV (single chain variable fragment) antibody prepared using the variable regions; (c) a method for preparing a recombinant scFv antibody; and (d) an expression vector for expressing a recombinant scFv antibody.

摘要翻译： 本发明涉及（a）艾美球虫子孢子中表面抗原特异性抗体的重链和轻链的可变区; （b）使用可变区制备的重组scFV（单链可变片段）抗体; （c）制备重组scFv抗体的方法; 和（d）用于表达重组scFv抗体的表达载体。

公开(公告)号：US20120245082A1

公开(公告)日：2012-09-27

申请号：US13071112

申请日：2011-03-24

申请人： Hyun Soon Lillehoj ,  Sung Hyen Lee ,  Yeong ho Hong

发明人： Hyun Soon Lillehoj ,  Sung Hyen Lee ,  Yeong ho Hong

IPC分类号： A61K38/17 ,  A61P33/02 ,  C07K14/465

CPC分类号： C07K14/465 ,  A61K38/17 ,  C07K14/52

摘要： NK-2 synthetic peptide derived from cationic core region of porcine NK-lysin has an antimicrobial and antitumor polypeptide that is considered to play a pivotal role in innate defense immunity. To evaluate the antimicrobial properties of synthetic chicken NK-lysin peptides, we investigated cytolytic activity against apicomplexan parasites like Eimeria sporozoites, Neospora tachyzoites and Cryptosporidum sporozoites. The chicken NK-2 (cNK-2) lytic peptide which corresponds to amino acid residues of porcine NK-2 peptide significantly disrupted Eimeria and Cryptosporidum sporozoites and Neospora tachyzoites. In contrast, no bactericidal activity was observed on E. coli BMH71-18. The cNK-2 lytic peptide is novel antimicrobial agent which can be used to intervene and treat economically costly infections in the animal industry.

摘要翻译： 来源于猪NK-lysin的阳离子核心区域的NK-2合成肽具有抗原和抗肿瘤多肽，被认为在先天免疫中具有关键作用。 为了评估合成鸡NK-赖氨酸肽的抗菌性能，我们研究了针对致密虫寄生虫如艾美球虫子孢子，新孢子虫速殖子和隐孢子虫子孢子的溶细胞活性。 对应于NK-2肽氨基酸残基的鸡NK-2（cNK-2）裂解肽显着破坏艾美球虫和隐孢子虫子孢子和新孢子虫速殖子。 相反，在大肠杆菌BMH71-18上没有观察到杀菌活性。 cNK-2裂解肽是新型抗菌剂，可用于干预和治疗动物行业经济上昂贵的感染。

公开(公告)号：US08691943B2

公开(公告)日：2014-04-08

申请号：US13071112

申请日：2011-03-24

申请人： Hyun Soon Lillehoj ,  Sung Hyen Lee ,  Yeong ho Hong

发明人： Hyun Soon Lillehoj ,  Sung Hyen Lee ,  Yeong ho Hong

IPC分类号： A61K38/00 ,  C07K5/00 ,  C07K7/00 ,  C07K16/00 ,  C07K17/00 ,  A61K38/04 ,  C07K1/00 ,  A01N37/18 ,  A61P33/02 ,  A61K51/00

CPC分类号： C07K14/465 ,  A61K38/17 ,  C07K14/52

摘要： NK-2 synthetic peptide derived from cationic core region of porcine NK-lysin has an antimicrobial and antitumor polypeptide that is considered to play a pivotal role in innate defense immunity. To evaluate the antimicrobial properties of synthetic chicken NK-lysin peptides, we investigated cytolytic activity against apicomplexan parasites like Eimeria sporozoites, Neospora tachyzoites and Cryptosporidum sporozoites. The chicken NK-2 (cNK-2) lytic peptide which corresponds to amino acid residues of porcine NK-2 peptide significantly disrupted Eimeria and Cryptosporidum sporozoites and Neospora tachyzoites. In contrast, no bactericidal activity was observed on E. coli BMH71-18. The cNK-2 lytic peptide is novel antimicrobial agent which can be used to intervene and treat economically costly infections in the animal industry.

摘要翻译： 来源于猪NK-lysin的阳离子核心区域的NK-2合成肽具有抗原和抗肿瘤多肽，被认为在先天免疫中具有关键作用。 为了评估合成鸡NK-赖氨酸肽的抗菌性能，我们研究了针对致密虫寄生虫如艾美球虫子孢子，新孢子虫速殖子和隐孢子虫子孢子的溶细胞活性。 对应于NK-2肽氨基酸残基的鸡NK-2（cNK-2）裂解肽显着破坏艾美球虫和隐孢子虫子孢子和新孢子虫速殖子。 相反，在大肠杆菌BMH71-18上没有观察到杀菌活性。 cNK-2裂解肽是新型抗菌剂，可用于干预和治疗动物行业经济上昂贵的感染。