公开(公告)号：US08809300B2

公开(公告)日：2014-08-19

申请号：US13512142

申请日：2010-10-25

申请人： Jinhua Zhao ,  Hui Kang ,  Mingyi Wu ,  Weizhen Zeng ,  Zi Li ,  Yuan Gao ,  Jing Cui ,  Zhiguo Wang ,  Hanlin Feng ,  Lin Yu

发明人： Jinhua Zhao ,  Hui Kang ,  Mingyi Wu ,  Weizhen Zeng ,  Zi Li ,  Yuan Gao ,  Jing Cui ,  Zhiguo Wang ,  Hanlin Feng ,  Lin Yu

IPC分类号： A61K31/726 ,  A61K31/715 ,  C08B37/00 ,  C07H5/06 ,  A61P7/02 ,  A61K31/737

CPC分类号： A61K31/737 ,  A61K31/726 ,  C08B37/0063

摘要： Disclosed is a depolymerized glycosaminoglycan from Thelenota ananas (dTHG), weight average molecular weight of which is about 8000˜20000 Da, and monosaccharide components of which are acetylgalactosamine (GalNAc), glucuronic acid (GlcUA), fucose (Fuc) or their sulfates (expressed as —OSO3−), in which molar ratio of GalNAc:GlcUA:Fuc:—OSO3− is about 1:(1±0.3):(1±0.3):(3.5±0.5). Said dTHG is a potent endogenous inhibitor of factor X, which has good anticoagulant and antithrombotic activity, and can be used for the prevention and/or treatment of thrombotic diseases. Also provided is a method for preparing said dTHG, which comprises steps of 1) extracting and obtaining fucosylated glycosaminoglycan (THG) from the body wall of Thelenota ananas; 2) depolymerizing THG to obtain dTHG by method of peroxide depolymerization or method of peroxide depolymerization catalyzed by catalyst of the fourth period transition metal ions; 3) removing impurities with lower and/or higher molecular weight in dTHG.

摘要翻译： 公开了来自Thelenota ananas（dTHG）的解聚糖胺聚糖（dTHG），其重均分子量为约8000-20000Da，其单糖组分为乙酰半乳糖胺（GalNAc），葡糖醛酸（GlcUA），岩藻糖（Fuc）或其硫酸盐 表达为-OSO3-），其中GalNAc：GlcUA：Fuc：-OSO3-的摩尔比为约1：（1±0.3）：（1±0.3）：（3.5±0.5）。 所述dTHG是一种有效的因子X的内源性抑制剂，其具有良好的抗凝血和抗血栓形成活性，可用于预防和/或治疗血栓性疾病。 还提供了一种制备所述dTHG的方法，其包括以下步骤：1）从Thelenota ananas的体壁提取并获得岩藻糖基化的糖胺聚糖（THG）; 2）解聚THG，通过过氧化物解聚法或第四周期过渡金属离子催化剂催化的过氧化物解聚方法得到dTHG; 3）去除dTHG中具有较低和/或更高分子量的杂质。

公开(公告)号：US20120270834A1

公开(公告)日：2012-10-25

申请号：US13512142

申请日：2010-10-25

申请人： Jinhua Zhao ,  Hui Kang ,  Mingyi Wu ,  Weizhen Zeng ,  Zi LI ,  Yuan Gao ,  Jing Cui ,  Zhiguo Wang ,  Hanlin Feng ,  Lin Yu

发明人： Jinhua Zhao ,  Hui Kang ,  Mingyi Wu ,  Weizhen Zeng ,  Zi LI ,  Yuan Gao ,  Jing Cui ,  Zhiguo Wang ,  Hanlin Feng ,  Lin Yu

IPC分类号： A61K31/726 ,  A61P7/02 ,  C08B37/00

CPC分类号： A61K31/737 ,  A61K31/726 ,  C08B37/0063

摘要： Disclosed is a depolymerized glycosaminoglycan from Thelenota ananas (dTHG), weight average molecular weight of which is about 8000˜20000 Da, and monosaccharide components of which are acetylgalactosamine (GalNAc), glucuronic acid (GlcUA), fucose (Fuc) or their sulfates (expressed as —OSO3−), in which molar ratio of GalNAc:GlcUA:Fuc:—OSO3− is about 1:(1±0.3):(1±0.3):(3.5±0.5). Said dTHG is a potent endogenous inhibitor of factor X, which has good anticoagulant and antithrombotic activity, and can be used for the prevention and/or treatment of thrombotic diseases. Also provided is a method for preparing said dTHG, which comprises steps of 1) extracting and obtaining fucosylated glycosaminoglycan (THG) from the body wall of Thelenota ananas; 2) depolymerizing THG to obtain dTHG by method of peroxide depolymerization or method of peroxide depolymerization catalyzed by catalyst of the fourth period transition metal ions; 3) removing impurities with lower and/or higher molecular weight in dTHG.

摘要翻译： 公开了来自Thelenota ananas（dTHG）的解聚糖胺聚糖（dTHG），其重均分子量为约8000-20000Da，其单糖组分为乙酰半乳糖胺（GalNAc），葡糖醛酸（GlcUA），岩藻糖（Fuc）或其硫酸盐 表达为-OSO3-），其中GalNAc：GlcUA：Fuc：-OSO3-的摩尔比为约1：（1±0.3）：（1±0.3）：（3.5±0.5）。 所述dTHG是一种有效的因子X的内源性抑制剂，其具有良好的抗凝血和抗血栓形成活性，可用于预防和/或治疗血栓性疾病。 还提供了一种制备所述dTHG的方法，其包括以下步骤：1）从Thelenota ananas的体壁提取并获得岩藻糖基化的糖胺聚糖（THG）; 2）解聚THG，通过过氧化物解聚法或第四周期过渡金属离子催化剂催化的过氧化物解聚方法得到dTHG; 3）去除dTHG中具有较低和/或更高分子量的杂质。