公开(公告)号：US20120178107A1

公开(公告)日：2012-07-12

申请号：US13340805

申请日：2011-12-30

申请人： Jochen G. Salfeld ,  Deborah J. Allen ,  Hendricus R.J.M. Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

发明人： Jochen G. Salfeld ,  Deborah J. Allen ,  Hendricus R.J.M. Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

IPC分类号： G01N33/566 ,  C07K16/24 ,  C12N9/96

CPC分类号： C07K16/241 ,  A61K38/00 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  Y02A50/412 ,  Y10S424/80 ,  Y10S424/81 ,  Y10S530/868

摘要： Human antibodies, preferably recombinant human antibodies, that specifically bind to human tumor necrosis factor α (hTNFα) are disclosed. These antibodies have high affinity for hTNFα (e.g., Kd=10−8 M or less), a slow off rate for hTNFα dissociation (e.g., Koff=10−3 sec−1 or less) and neutralize hTNFα activity in vitro and in vivo. An antibody of the invention can be a full-length antibody or an antigen-binding portion thereof. The antibodies, or antibody portions, of the invention are useful for detecting hTNFα and for inhibiting hTNFα activity, e.g., in a human subject suffering from a disorder in which hTNFα activity is detrimental. Nucleic acids, vectors and host cells for expressing the recombinant human antibodies of the invention, and methods of synthesizing the recombinant human antibodies, are also encompassed by the invention.

摘要翻译： 公开了特异性结合人肿瘤坏死因子α（hTNFα）的人抗体，优选重组人抗体。 这些抗体对hTNFα具有高亲和力（例如，Kd = 10-8M或更低），hTNFα解离的缓慢速率（例如，Koff = 10-3秒-1或更低）并且在体外和体内中和hTNFα活性 。 本发明的抗体可以是全长抗体或其抗原结合部分。 本发明的抗体或抗体部分可用于检测hTNFα和抑制hTNFα活性，例如在患有hTNFα活性有害的病症的人类受试者中。 用于表达本发明的重组人抗体的核酸，载体和宿主细胞以及合成重组人抗体的方法也包括在本发明中。

公开(公告)号：US08414894B2

公开(公告)日：2013-04-09

申请号：US13340805

申请日：2011-12-30

申请人： Jochen G. Salfeld ,  John A. Mankovich ,  Deborah J. Allen ,  Hendricus R.J.M. Hoogenboom ,  Boris Labkovsky ,  Brian T. McGuinness ,  Paul Sakorafas ,  Michael White ,  Alison J. Wilton ,  Zehra Kaymakcalan ,  Andrew J. Roberts ,  David Schoenhaut ,  Tristan J. Vaughan

发明人： Jochen G. Salfeld ,  John A. Mankovich ,  Deborah J. Allen ,  Hendricus R.J.M. Hoogenboom ,  Boris Labkovsky ,  Brian T. McGuinness ,  Paul Sakorafas ,  Michael White ,  Alison J. Wilton ,  Zehra Kaymakcalan ,  Andrew J. Roberts ,  David Schoenhaut ,  Tristan J. Vaughan

IPC分类号： A61K39/395 ,  C07K16/00 ,  G01N33/53

CPC分类号： C07K16/241 ,  A61K38/00 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  Y02A50/412 ,  Y10S424/80 ,  Y10S424/81 ,  Y10S530/868

摘要： Human antibodies, preferably recombinant human antibodies, that specifically bind to human tumor necrosis factor α (hTNFα) are disclosed. These antibodies have high affinity for hTNFα (e.g., Kd=10−8 M or less), a slow off rate for hTNFα dissociation (e.g., Koff=10−3 sec−1 or less) and neutralize hTNFα activity in vitro and in vivo. An antibody of the invention can be a full-length antibody or an antigen-binding portion thereof. The antibodies, or antibody portions, of the invention are useful for detecting hTNFα and for inhibiting hTNFα activity, e.g., in a human subject suffering from a disorder in which hTNFα activity is detrimental. Nucleic acids, vectors and host cells for expressing the recombinant human antibodies of the invention, and methods of synthesizing the recombinant human antibodies, are also encompassed by the invention.

摘要翻译： 公开了特异性结合人肿瘤坏死因子α（hTNFα）的人抗体，优选重组人抗体。 这些抗体对hTNFα具有高亲和力（例如，Kd = 10-8M或更低），hTNFα解离的缓慢速率（例如，Koff = 10-3秒-1或更低），并在体外和体内中和hTNFα活性 。 本发明的抗体可以是全长抗体或其抗原结合部分。 本发明的抗体或抗体部分可用于检测hTNFα和抑制hTNFα活性，例如在患有hTNFα活性有害的病症的人类受试者中。 用于表达本发明的重组人抗体的核酸，载体和宿主细胞以及合成重组人抗体的方法也包括在本发明中。

公开(公告)号：US20130115224A1

公开(公告)日：2013-05-09

申请号：US13736931

申请日：2013-01-08

申请人： Jochen G. SALFELD ,  Deborah J. Allen ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  Hendricus R.j.m. Hoogenboom ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

发明人： Jochen G. SALFELD ,  Deborah J. Allen ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  Hendricus R.j.m. Hoogenboom ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

IPC分类号： C07K16/24

CPC分类号： C07K16/241 ,  A61K38/00 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/565 ,  Y02A50/412 ,  Y10S424/81

摘要： Human antibodies, preferably recombinant human antibodies, that specifically bind to human tumor necrosis factor α (hTNFα) are disclosed. These antibodies have high affinity for hTNFα (e.g., Kd=10−8 M or less), a slow off rate for hTNFα dissociation (e.g., Koff=10−3 sec−1 or less) and neutralize hTNFα activity in vitro and in vivo. An antibody of the invention can be a full-length antibody or an antigen-binding portion thereof. The antibodies, or antibody portions, of the invention are useful for detecting hTNFα and for inhibiting hTNFα activity, e.g., in a human subject suffering from a disorder in which hTNFα activity is detrimental. Nucleic acids, vectors and host cells for expressing the recombinant human antibodies of the invention, and methods of synthesizing the recombinant human antibodies, are also encompassed by the invention.

摘要翻译： 公开了特异性结合人肿瘤坏死因子α（hTNFα）的人抗体，优选重组人抗体。 这些抗体对hTNFalpha（例如，Kd = 10-8M或更低）具有高亲和力，hTNFalpha解离的缓慢速率（例如，Koff = 10-3秒-1或更低），并在体外和体内中和hTNFalpha活性 。 本发明的抗体可以是全长抗体或其抗原结合部分。 本发明的抗体或抗体部分可用于检测hTNFα和抑制hTNFα活性，例如在患有hTNFα活性有害的病症的人类受试者中。 用于表达本发明的重组人抗体的核酸，载体和宿主细胞以及合成重组人抗体的方法也包括在本发明中。

公开(公告)号：US20090155205A1

公开(公告)日：2009-06-18

申请号：US12369451

申请日：2009-02-11

申请人： Jochen G. Salfeld ,  Deborah J. Allen ,  Hendricus R.J.M Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

发明人： Jochen G. Salfeld ,  Deborah J. Allen ,  Hendricus R.J.M Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

IPC分类号： A61K38/20 ,  A61K39/395 ,  A61K38/21 ,  A61P37/00

CPC分类号： C07K16/241 ,  A61K38/00 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/565 ,  Y02A50/412 ,  Y10S424/81

摘要： Human antibodies, preferably recombinant human antibodies, that specifically bind to human tumor necrosis factor α (hTNFα) are disclosed. These antibodies have high affinity for hTNFα (e.g., Kd=10−8 M or less), a slow off rate for hTNFα dissociation (e.g., Koff=10−3 sec−1 or less) and neutralize hTNFα activity in vitro and in vivo. An antibody of the invention can be a full-length antibody or an antigen-binding portion thereof. The antibodies, or antibody portions, of the invention are useful for detecting hTNFα and for inhibiting hTNFα activity, e.g., in a human subject suffering from a disorder in which hTNFα activity is detrimental. Nucleic acids, vectors and host cells for expressing the recombinant human antibodies of the invention, and methods of synthesizing the recombinant human antibodies, are also encompassed by the invention.

摘要翻译： 公开了特异性结合人肿瘤坏死因子α（hTNFα）的人抗体，优选重组人抗体。 这些抗体对hTNFalpha（例如，Kd = 10-8M或更低）具有高亲和力，hTNFalpha解离的缓慢速率（例如，Koff = 10-3秒-1或更低），并在体外和体内中和hTNFalpha活性 。 本发明的抗体可以是全长抗体或其抗原结合部分。 本发明的抗体或抗体部分可用于检测hTNFα和抑制hTNFα活性，例如在患有hTNFα活性有害的病症的人类受试者中。 用于表达本发明的重组人抗体的核酸，载体和宿主细胞以及合成重组人抗体的方法也包括在本发明中。

公开(公告)号：US20100040604A1

公开(公告)日：2010-02-18

申请号：US12578487

申请日：2009-10-13

申请人： Jochen G. SALFELD ,  Deborah J. Allen ,  Hendricus R.J.M. Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

发明人： Jochen G. SALFELD ,  Deborah J. Allen ,  Hendricus R.J.M. Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

IPC分类号： A61K39/395

CPC分类号： C07K16/241 ,  A61K38/00 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/565 ,  Y02A50/412 ,  Y10S424/81

摘要： Human antibodies, preferably recombinant human antibodies, that specifically bind to human tumor necrosis factor α (hTNFα) are disclosed. These antibodies have high affinity for hTNFα (e.g., Kd=10−8 M or less), a slow off rate for hTNFα dissociation (e.g., Koff=10−3 sec−1 or less) and neutralize hTNFα activity in vitro and in vivo. An antibody of the invention can be a full-length antibody or an antigen-binding portion thereof. The antibodies, or antibody portions, of the invention are useful for detecting hTNFα and for inhibiting hTNFα activity, e.g., in a human subject suffering from a disorder in which hTNFα activity is detrimental. Nucleic acids, vectors and host cells for expressing the recombinant human antibodies of the invention, and methods of synthesizing the recombinant human antibodies, are also encompassed by the invention.

摘要翻译： 公开了特异性结合人肿瘤坏死因子α（hTNFα）的人抗体，优选重组人抗体。 这些抗体对hTNFα具有高亲和力（例如，Kd = 10-8M或更低），hTNFα解离的缓慢速率（例如，Koff = 10-3秒-1或更低），并在体外和体内中和hTNFα活性 。 本发明的抗体可以是全长抗体或其抗原结合部分。 本发明的抗体或抗体部分可用于检测hTNFα和抑制hTNFα活性，例如在患有hTNFα活性有害的病症的人类受试者中。 用于表达本发明的重组人抗体的核酸，载体和宿主细胞以及合成重组人抗体的方法也包括在本发明中。

公开(公告)号：US20100016557A1

公开(公告)日：2010-01-21

申请号：US12401469

申请日：2009-03-10

申请人： Jochen G. Salfeld ,  Deborah J. Allen ,  Hendricus R.J.M. Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

发明人： Jochen G. Salfeld ,  Deborah J. Allen ,  Hendricus R.J.M. Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

IPC分类号： C07K16/24 ,  C07H21/04

CPC分类号： C07K16/241 ,  A61K38/00 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  Y02A50/412 ,  Y10S424/80 ,  Y10S424/81 ,  Y10S530/868

摘要： Human antibodies, preferably recombinant human antibodies, that specifically bind to human tumor necrosis factor α (hTNFα) are disclosed. These antibodies have high affinity for hTNFα (e.g., Kd=10−8 M or less), a slow off rate for hTNFα dissociation (e.g., Koff=10−3 sec−1 or less) and neutralize hTNFα activity in vitro and in vivo. An antibody of the invention can be a full-length antibody or an antigen-binding portion thereof. The antibodies, or antibody portions, of the invention are useful for detecting hTNFα and for inhibiting hTNFα activity, e.g., in a human subject suffering from a disorder in which hTNFα activity is detrimental. Nucleic acids, vectors and host cells for expressing the recombinant human antibodies of the invention, and methods of synthesizing the recombinant human antibodies, are also encompassed by the invention.

摘要翻译： 公开了特异性结合人肿瘤坏死因子α（hTNFα）的人抗体，优选重组人抗体。 这些抗体对hTNFalpha（例如，Kd = 10-8M或更低）具有高亲和力，hTNFalpha解离的缓慢速率（例如，Koff = 10-3秒-1或更低），并在体外和体内中和hTNFalpha活性 。 本发明的抗体可以是全长抗体或其抗原结合部分。 本发明的抗体或抗体部分可用于检测hTNFα和抑制hTNFα活性，例如在患有hTNFα活性有害的病症的人类受试者中。 用于表达本发明的重组人抗体的核酸，载体和宿主细胞以及合成重组人抗体的方法也包括在本发明中。

公开(公告)号：US06258562B1

公开(公告)日：2001-07-10

申请号：US09125098

申请日：1999-03-16

申请人： Jochen G. Salfeld ,  Deborah J. Allen ,  Hendricus R. J. M. Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

发明人： Jochen G. Salfeld ,  Deborah J. Allen ,  Hendricus R. J. M. Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

IPC分类号： C07M2100

CPC分类号： C07K16/241 ,  A61K38/00 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/565 ,  Y02A50/412 ,  Y10S424/81

摘要： Human antibodies, preferably recombinant human antibodies, that specifically bind to human tumor necrosis factor &agr;(hTNF&agr;) are disclosed. These antibodies have high affinity for hTNF&agr; (e.g., Kd=10−8 M or less), a slow off rate for hTNF&agr; dissociation (e.g., Koff=10−3sec−1 or less) and neutralize hTNF&agr; activity in vitro and in vivo. An antibody of the invention can be a full-length antibody or an antigen-binding portion thereof. The antibodies, or antibody portions, of the invention are useful for detecting hTNF&agr; and for inhibiting hTNF&agr; activity, e.g., in a human subject suffering from a disorder in which hTNF&agr; activity is detrimental. Nucleic acids, vectors and host cells for expressing the recombinant human antibodies of the invention, and methods of synthesizing the recombinant human antibodies, are also encompassed by the invention.

摘要翻译： 公开了特异性结合人肿瘤坏死因子α（hTNFα）的人抗体，优选重组人抗体。 这些抗体对hTNFα（例如，Kd = 10-8M或更低）具有高亲和力，hTNFalpha解离的缓慢速率（例如Koff = 10-3sec-1或更低）并且在体外和体内中和hTNFα活性。 本发明的抗体可以是全长抗体或其抗原结合部分。 本发明的抗体或抗体部分可用于检测hTNFα和抑制hTNFα活性，例如在患有hTNFα活性有害的病症的人类受试者中。 用于表达本发明的重组人抗体的核酸，载体和宿主细胞以及合成重组人抗体的方法也包括在本发明中。

公开(公告)号：US08197813B2

公开(公告)日：2012-06-12

申请号：US12401469

申请日：2009-03-10

申请人： Jochen G. Salfeld ,  Deborah J. Allen ,  Hendricus R. J. M. Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

发明人： Jochen G. Salfeld ,  Deborah J. Allen ,  Hendricus R. J. M. Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

IPC分类号： A61K39/395 ,  C07K16/24

CPC分类号： C07K16/241 ,  A61K38/00 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  Y02A50/412 ,  Y10S424/80 ,  Y10S424/81 ,  Y10S530/868

摘要： Human antibodies, preferably recombinant human antibodies, that specifically bind to human tumor necrosis factor α (hTNFα) are disclosed. These antibodies have high affinity for hTNFα (e.g., Kd=10−8 M or less), a slow off rate for hTNFα dissociation (e.g., Koff=10−3 sec−1 or less) and neutralize hTNFα activity in vitro and in vivo. An antibody of the invention can be a full-length antibody or an antigen-binding portion thereof. The antibodies, or antibody portions, of the invention are useful for detecting hTNFα and for inhibiting hTNFα activity, e.g., in a human subject suffering from a disorder in which hTNFα activity is detrimental. Nucleic acids, vectors and host cells for expressing the recombinant human antibodies of the invention, and methods of synthesizing the recombinant human antibodies, are also encompassed by the invention.

摘要翻译： 公开了特异性结合人肿瘤坏死因子α（hTNFα）的人抗体，优选重组人抗体。 这些抗体对hTNFα具有高亲和力（例如，Kd = 10-8M或更低），hTNFα解离的缓慢速率（例如，Koff = 10-3秒-1或更低）并且在体外和体内中和hTNFα活性 。 本发明的抗体可以是全长抗体或其抗原结合部分。 本发明的抗体或抗体部分可用于检测hTNFα和抑制hTNFα活性，例如在患有hTNFα活性有害的病症的人类受试者中。 用于表达本发明的重组人抗体的核酸，载体和宿主细胞以及合成重组人抗体的方法也包括在本发明中。

公开(公告)号：US08372401B2

公开(公告)日：2013-02-12

申请号：US13466892

申请日：2012-05-08

申请人： Jochen G. Salfeld ,  Deborah J. Allen ,  Hendricus R. J. M. Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

发明人： Jochen G. Salfeld ,  Deborah J. Allen ,  Hendricus R. J. M. Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

IPC分类号： A61K39/395 ,  C07K16/00

CPC分类号： C07K16/241 ,  A61K38/00 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  Y02A50/412 ,  Y10S424/80 ,  Y10S424/81 ,  Y10S530/868

摘要： Human antibodies, preferably recombinant human antibodies, that specifically bind to human tumor necrosis factor α (hTNFα) are disclosed. These antibodies have high affinity for hTNFα (e.g., Kd=10−8 M or less), a slow off rate for hTNFα dissociation (e.g., Koff=10−3 sec−1 or less) and neutralize hTNFα activity in vitro and in vivo. An antibody of the invention can be a full-length antibody or an antigen-binding portion thereof. The antibodies, or antibody portions, of the invention are useful for detecting hTNFα and for inhibiting hTNFα activity, e.g., in a human subject suffering from a disorder in which hTNFα activity is detrimental. Nucleic acids, vectors and host cells for expressing the recombinant human antibodies of the invention, and methods of synthesizing the recombinant human antibodies, are also encompassed by the invention.

摘要翻译： 公开了特异性结合人肿瘤坏死因子α（hTNFα）的人抗体，优选重组人抗体。 这些抗体对hTNFα具有高亲和力（例如，Kd = 10-8M或更低），hTNFα解离的缓慢速率（例如，Koff = 10-3秒-1或更低），并在体外和体内中和hTNFα活性 。 本发明的抗体可以是全长抗体或其抗原结合部分。 本发明的抗体或抗体部分可用于检测hTNFα和抑制hTNFα活性，例如在患有hTNFα活性有害的病症的人类受试者中。 用于表达本发明的重组人抗体的核酸，载体和宿主细胞以及合成重组人抗体的方法也包括在本发明中。

公开(公告)号：US06509015B1

公开(公告)日：2003-01-21

申请号：US09540018

申请日：2000-03-31

申请人： Jochen G. Salfeld ,  Deborah J. Allen ,  Hendricus R. J. M. Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

发明人： Jochen G. Salfeld ,  Deborah J. Allen ,  Hendricus R. J. M. Hoogenboom ,  Zehra Kaymakcalan ,  Boris Labkovsky ,  John A. Mankovich ,  Brian T. McGuinness ,  Andrew J. Roberts ,  Paul Sakorafas ,  David Schoenhaut ,  Tristan J. Vaughan ,  Michael White ,  Alison J. Wilton

IPC分类号： A61K39395

CPC分类号： C07K16/241 ,  A61K38/00 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  Y02A50/412 ,  Y10S424/80 ,  Y10S424/81 ,  Y10S530/868

摘要： Human antibodies, preferably recombinant human antibodies, that specifically bind to human tumor necrosis factor &agr; (hTNF&agr;) are disclosed. These antibodies have high affinity for hTNF&agr; (e.g., Kd=10−8 M or less), a slow off rate for hTNF&agr; dissociation (e.g., Koff=10−3 sec−1 or less) and neutralize hTNF&agr; activity in vitro and in vivo. An antibody of the invention can be a full-length antibody or an antigen-binding portion thereof. The antibodies, or antibody portions, of the invention are useful for detecting hTNF&agr; and for inhibiting hTNF&agr; activity, e.g., in a human subject suffering from a disorder in which hTNF&agr; activity is detrimental. Nucleic acids, vectors and host cells for expressing the recombinant human antibodies of the invention, and methods of synthesizing the recombinant human antibodies, are also encompassed by the invention.

摘要翻译： 公开了特异性结合人肿瘤坏死因子α（hTNFα）的人抗体，优选重组人抗体。 这些抗体对hTNFalpha（例如，Kd = 10-8M或更低）具有高亲和力，hTNFalpha解离的缓慢速率（例如，Koff = 10-3秒-1或更低），并在体外和体内中和hTNFalpha活性 。 本发明的抗体可以是全长抗体或其抗原结合部分。 本发明的抗体或抗体部分可用于检测hTNFα和抑制hTNFα活性，例如在患有hTNFα活性有害的病症的人类受试者中。 用于表达本发明的重组人抗体的核酸，载体和宿主细胞以及合成重组人抗体的方法也包括在本发明中。