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4 条记录 (耗时 0.048 秒)

    DNA encoding plant chitinases
    1.
    发明授权
    DNA encoding plant chitinases 失效
    编码植物几丁质酶的DNA

    公开(公告)号：US5880328A

    公开(公告)日：1999-03-09

    申请号：US455736

    申请日：1995-05-31

    申请人： John A. Ryals ,  Eric R. Ward ,  George B. Payne ,  Mary B. Moyer ,  Frederich Meins, Jr.

    发明人： John A. Ryals ,  Eric R. Ward ,  George B. Payne ,  Mary B. Moyer ,  Frederich Meins, Jr.

    IPC分类号： C07D285/14 ,  C07K14/415 ,  C12N9/02 ,  C12N9/08 ,  C12N9/24 ,  C12N9/36 ,  C12N15/29 ,  C12N15/56 ,  C12N15/62 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N9/2462 ,  C07D285/14 ,  C07K14/415 ,  C12N15/625 ,  C12N15/8238 ,  C12N15/8239 ,  C12N15/8274 ,  C12N15/8282 ,  C12N15/8286 ,  C12N9/0065 ,  C12N9/0073 ,  C12N9/2442 ,  C12Y114/13001 ,  C12Y302/01014 ,  C12Y302/01039 ,  C12Y302/01058 ,  C12Y302/01073 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/55 ,  C07K2319/61

    摘要： The present invention provides chemically regulatable DNA sequences capable of regulating transcription of an associated DNA sequence in plants or plant tissues, chimeric constructions containing such sequences, vectors containing such sequences and chimeric constructions, and transgenic plants and plant tissues containing these chimeric constructions. In one aspect, the chemically regulatable DNA sequences of the invention are derived from the 5' region of genes encoding pathogenisis-related (PR) proteins. The present invention also provides anti-pathogenic sequences derived from novel cDNAs coding for PR proteins which can be genetically engineered and transformed into plants to confer enhanced resistance to disease. Also provided is a method for the exogenous regulation of gene expression in plants, which comprises obtaining a plant incapable of regulating at least one gene or gene family, or at least one heterologous gene, due to the deactivation of at least one endogenous signal transduction cascade which regulates the gene in the plant, and applying a chemical regulator to the plant at a time when expression of the gene is desired. A novel signal peptide sequence and corresponding DNA coding sequence is also provided. Further provided are assays for the identification and isolation of additional chemically regulatable DNA sequences and cDNAs encoding PR proteins and assays for identifying chemicals capable of exogenously regulating the chemically regulatable DNA sequences of the invention.

    摘要翻译： 本发明提供能够调节植物或植物组织中相关DNA序列转录的化学可调节DNA序列，含有此类序列的嵌合构建体，含有此类序列的载体和嵌合构建体，以及含有这些嵌合构建体的转基因植物和植物组织。 在一个方面，本发明的化学可调节DNA序列衍生自编码发病相关（PR）蛋白的基因的5'区域。 本发明还提供衍生自编码PR蛋白的新cDNA的抗病原性序列，其可以被遗传工程化并转化成植物以赋予增强的抗病性。 还提供了外源调节植物中基因表达的方法，其包括获得不能调节至少一个基因或基因家族或至少一个异源基因的植物，因为至少一种内源性信号转导级联失活 其调节植物中的基因，并且在需要表达基因的时候将化学调节剂施用于植物。 还提供了新的信号肽序列和相应的DNA编码序列。 进一步提供用于鉴定和分离另外的化学可调节DNA序列和编码PR蛋白的cDNA和用于鉴定能够外源调节本发明的化学可调节DNA序列的化学品的测定。

    DNA encoding .beta.-1,3-glucanases
    4.
    发明授权
    DNA encoding .beta.-1,3-glucanases 失效
    编码β-1,3-葡聚糖酶的DNA

    公开(公告)号：US5847258A

    公开(公告)日：1998-12-08

    申请号：US457364

    申请日：1995-05-31

    申请人： John A. Ryals ,  Mary B. Moyer ,  George B. Payne ,  Eric R. Ward

    发明人： John A. Ryals ,  Mary B. Moyer ,  George B. Payne ,  Eric R. Ward

    IPC分类号： C07D285/14 ,  C07K14/415 ,  C12N9/02 ,  C12N9/08 ,  C12N9/24 ,  C12N9/36 ,  C12N15/29 ,  C12N15/56 ,  C12N15/62 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N9/2462 ,  C07D285/14 ,  C07K14/415 ,  C12N15/625 ,  C12N15/8238 ,  C12N15/8239 ,  C12N15/8274 ,  C12N15/8282 ,  C12N15/8286 ,  C12N9/0065 ,  C12N9/0073 ,  C12N9/2442 ,  C12Y114/13001 ,  C12Y302/01014 ,  C12Y302/01039 ,  C12Y302/01058 ,  C12Y302/01073 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/55 ,  C07K2319/61

    摘要： The present invention provides chemically regulatable DNA sequences capable of regulating transcription of an associated DNA sequence in plants or plant tissues, chimeric constructions containing such sequences, vectors containing such sequences and chimeric constructions, and transgenic plants and plant tissues containing these chimeric constructions. In one aspect, the chemically regulatable DNA sequences of the invention are derived from the 5' region of genes encoding pathogenisis-related (PR) proteins. The present invention also provides anti-pathogenic sequences derived from novel cDNAs coding for PR proteins which can be genetically engineered and transformed into plants to confer enhanced resistance to disease. Also provided is a method for the exogenous regulation of gene expression in plants, which comprises obtaining a plant incapable of regulating at least one gene or gene family, or at least one heterologous gene, due to the deactivation of at least one endogenous signal transduction cascade which regulates the gene in the plant, and applying a chemical regulator to the plant at a time when expression of the gene is desired. A novel signal peptide sequence and corresponding DNA coding sequence is also provided. Further provided are assays for the identification and isolation of additional chemically regulatable DNA sequences and cDNAs encoding PR proteins and assays for identifying chemicals capable of exogenously regulating the chemically regulatable DNA sequences of the invention.

    摘要翻译： 本发明提供能够调节植物或植物组织中相关DNA序列转录的化学可调节DNA序列，含有此类序列的嵌合构建体，含有此类序列的载体和嵌合构建体，以及含有这些嵌合构建体的转基因植物和植物组织。 在一个方面，本发明的化学可调节DNA序列衍生自编码发病相关（PR）蛋白的基因的5'区域。 本发明还提供衍生自编码PR蛋白的新cDNA的抗病原性序列，其可以被遗传工程化并转化成植物以赋予增强的抗病性。 还提供了外源调节植物中基因表达的方法，其包括获得不能调节至少一个基因或基因家族或至少一个异源基因的植物，因为至少一种内源性信号转导级联失活 其调节植物中的基因，并且在需要表达基因的时候将化学调节剂施用于植物。 还提供了新的信号肽序列和相应的DNA编码序列。 进一步提供用于鉴定和分离另外的化学可调节DNA序列和编码PR蛋白的cDNA和用于鉴定能够外源调节本发明的化学可调节DNA序列的化学品的测定。