公开(公告)号：US20130146815A1

公开(公告)日：2013-06-13

申请号：US13607337

申请日：2012-09-07

申请人： John Hagen Pryce BAYLEY ,  Matthew Holden ,  Andrew John Heron ,  David Needham

发明人： John Hagen Pryce BAYLEY ,  Matthew Holden ,  Andrew John Heron ,  David Needham

IPC分类号： G01N33/68

CPC分类号： G01N33/6872 ,  G01N33/5432 ,  G01N2333/31 ,  Y10T436/12

摘要： A method of forming bilayers of amphipathic molecules uses droplets of aqueous solution in a hydrophobic medium such as oil. A layer of amphipathic molecules such as a lipid is formed around the surfaces of the droplets. This may be achieved by providing the lipid in the oil and leaving the droplets for a time sufficient to form the layer. The droplets are brought into contact with one another so that a bilayer of the amphipathic molecules is formed as an interface between the contacting droplets. The bilayers may be used for a wide range of studies. The technique has numerous advantages including providing a long lifetime for the bilayers, allowing study of small volumes and allowing the construction of chains and networks of droplets with bilayers in between to study complex systems.

摘要翻译： 形成两亲分子双层的方法使用疏水介质如油中的水溶液滴。 在液滴的表面周围形成一层两亲性分子，例如脂质。 这可以通过在油中提供脂质并将液滴留下足以形成该层的时间来实现。 使液滴彼此接触，使得两亲分子的双层形成为接触液滴之间的界面。 双层可用于广泛的研究。 该技术具有许多优点，包括为双层提供长寿命，允许研究小体积，并允许在双层之间建立具有双层的液滴链和网络以研究复杂系统。

公开(公告)号：US08691586B2

公开(公告)日：2014-04-08

申请号：US13607337

申请日：2012-09-07

申请人： John Hagen Pryce Bayley ,  Matthew Holden ,  Andrew John Heron ,  David Needham

发明人： John Hagen Pryce Bayley ,  Matthew Holden ,  Andrew John Heron ,  David Needham

IPC分类号： G01N33/68

CPC分类号： G01N33/6872 ,  G01N33/5432 ,  G01N2333/31 ,  Y10T436/12

摘要： A method of forming bilayers of amphipathic molecules uses droplets of aqueous solution in a hydrophobic medium such as oil. A layer of amphipathic molecules such as a lipid is formed around the surfaces of the droplets. This may be achieved by providing the lipid in the oil and leaving the droplets for a time sufficient to form the layer. The droplets are brought into contact with one another so that a bilayer of the amphipathic molecules is formed as an interface between the contacting droplets. The bilayers may be used for a wide range of studies. The technique has numerous advantages including providing a long lifetime for the bilayers, allowing study of small volumes and allowing the construction of chains and networks of droplets with bilayers in between to study complex systems.

摘要翻译： 形成两亲分子双层的方法使用疏水介质如油中的水溶液滴。 在液滴的表面周围形成一层两亲性分子，例如脂质。 这可以通过在油中提供脂质并将液滴留下足以形成该层的时间来实现。 使液滴彼此接触，使得两亲分子的双层形成为接触液滴之间的界面。 双层可用于广泛的研究。 该技术具有许多优点，包括为双层提供长寿命，允许研究小体积，并允许在双层之间建立链条和液滴网络以研究复杂系统。

公开(公告)号：US20100032627A1

公开(公告)日：2010-02-11

申请号：US12375103

申请日：2007-07-26

申请人： John Hagen Pryce Bayley ,  Matthew Holden ,  Andrew John Heron ,  David Needham

发明人： John Hagen Pryce Bayley ,  Matthew Holden ,  Andrew John Heron ,  David Needham

IPC分类号： G01N27/00

CPC分类号： G01N33/6872 ,  G01N33/5432 ,  G01N2333/31 ,  Y10T436/12

摘要： A method of forming bilayers of amphipathic molecules uses droplets of aqueous solution in a hydrophobic medium such as oil. A layer of amphipathic molecules such as a lipid is formed around the surfaces of the droplets. This may be achieved by providing the lipid in the oil and leaving the droplets for a time sufficient to form the layer. The droplets are brought into contact with one another so that a bilayer of the amphipathic molecules is formed as an interface between the contacting droplets. The bilayers may be used for a wide range of studies. The technique has numerous advantages including providing a long lifetime for the bilayers, allowing study of small volumes and allowing the construction of chains and networks of droplets with bilayers in between to study complex systems.

摘要翻译： 形成两亲分子双层的方法使用疏水介质如油中的水溶液滴。 在液滴的表面周围形成一层两亲性分子，例如脂质。 这可以通过在油中提供脂质并将液滴留下足以形成该层的时间来实现。 使液滴彼此接触，使得两亲分子的双层形成为接触液滴之间的界面。 双层可用于广泛的研究。 该技术具有许多优点，包括为双层提供长寿命，允许研究小体积，并允许在双层之间建立链条和液滴网络以研究复杂系统。

公开(公告)号：US08268627B2

公开(公告)日：2012-09-18

申请号：US12375103

申请日：2007-07-26

申请人： John Hagen Pryce Bayley ,  Matthew Holden ,  Andrew John Heron ,  David Needham

发明人： John Hagen Pryce Bayley ,  Matthew Holden ,  Andrew John Heron ,  David Needham

IPC分类号： G01N33/92

CPC分类号： G01N33/6872 ,  G01N33/5432 ,  G01N2333/31 ,  Y10T436/12

摘要： A method of forming bilayers of amphipathic molecules uses droplets of aqueous solution in a hydrophobic medium such as oil. A layer of amphipathic molecules such as a lipid is formed around the surfaces of the droplets. This may be achieved by providing the lipid in the oil and leaving the droplets for a time sufficient to form the layer. The droplets are brought into contact with one another so that a bilayer of the amphipathic molecules is formed as an interface between the contacting droplets. The bilayers may be used for a wide range of studies. The technique has numerous advantages including providing a long lifetime for the bilayers, allowing study of small volumes and allowing the construction of chains and networks of droplets with bilayers in between to study complex systems.

摘要翻译： 形成两亲分子双层的方法使用疏水介质如油中的水溶液滴。 在液滴的表面周围形成一层两亲性分子，例如脂质。 这可以通过在油中提供脂质并将液滴留下足以形成该层的时间来实现。 使液滴彼此接触，使得两亲分子的双层形成为接触液滴之间的界面。 双层可用于广泛的研究。 该技术具有许多优点，包括为双层提供长寿命，允许研究小体积，并允许在双层之间建立链条和液滴网络以研究复杂系统。