公开(公告)号：US06403354B1

公开(公告)日：2002-06-11

申请号：US09766055

申请日：2001-01-19

申请人： Shuang-yong Xu ,  James Samuelson ,  John Pelletier ,  Marion Sibley ,  Geoffrey G. Wilson

发明人： Shuang-yong Xu ,  James Samuelson ,  John Pelletier ,  Marion Sibley ,  Geoffrey G. Wilson

IPC分类号： C12N922

CPC分类号： C12N9/22 ,  C12N9/1007

摘要： The present invention relates to recombinant DNA which encodes the BstYI restriction endonuclease as well as BstYI methyltransferase, expression of BstYI restriction endonuclease and M.BstYI in E. coli cells containing the recombinant DNA. It also relates to methods for purification of the recombinant BstYI restriction endonuclease and BstYI methyltransferase.

摘要翻译： 本发明涉及编码BstYI限制性内切核酸酶以及BstYI甲基转移酶，BstYI限制性内切核酸酶和M.BstYI在含有重组DNA的大肠杆菌细胞中的表达的重组DNA。 它还涉及重组BstYI限制性内切核酸酶和BstYI甲基转移酶的纯化方法。

公开(公告)号：US3998028A

公开(公告)日：1976-12-21

申请号：US573829

申请日：1975-05-02

申请人： John Pelletier ,  Eugene H. Soucy

发明人： John Pelletier ,  Eugene H. Soucy

IPC分类号： E04B1/94 ,  E04C3/30

CPC分类号： E04B1/944

摘要： A clip assembly for attaching a member, such as a partition or duct, to a support covered with a layer of a fireproofing material while maintaining the integrity of the fireproofing layer, or attaching wallboard to a beam or column, has a pair of clip sub-assemblies adjustably connectible together to fit different size supports. Each clip sub-assembly is provided with a plate portion defining a planar support engaging surface substantially in the form of the outline of a right triangle and arranged particularly for gripping a flange of an I-beam and the like, while end walls perpendicular to the plate portions facilitate attachment of wallboard to the clip assembly for furring, and the like. Base portions, one of which has a slot and the other a plurality of spaced openings mateable to the slot, are connected to the plate portions and permit the adjustable connection of the clip sub-assemblies to various size supports.

摘要翻译： 用于将诸如隔板或管道的构件附接到覆盖有防火材料层的支撑件的同时保持防火层的完整性或将墙板附接到梁或柱的夹子组件具有一对夹子 - 组件可调节地连接在一起以适应不同尺寸的支撑。 每个夹子子组件设置有限定基本上呈直角三角形轮廓形式的平面支撑接合表面的板部分，特别用于夹紧I型梁等的凸缘，同时垂直于 板部分便于将墙板附接到夹子组件以进行毛边等。 基座部分中的一个具有狭槽，而另一个具有与槽相配的多个间隔的开口连接到板部分，并且允许夹子子组件可调节地连接到各种尺寸的支撑件。

公开(公告)号：US06764843B2

公开(公告)日：2004-07-20

申请号：US09966997

申请日：2001-09-28

申请人： Shuang-yong Xu ,  Andrew Dore ,  Adam Hume ,  John Pelletier ,  Jing Zhou

发明人： Shuang-yong Xu ,  Andrew Dore ,  Adam Hume ,  John Pelletier ,  Jing Zhou

IPC分类号： C12N922

CPC分类号： C07K14/32 ,  C12N9/1007 ,  C12N9/22

摘要： The present invention relates to recombinant DNA that encodes the BsmBI restriction endonuclease as well as BsmBI methyltransferase, expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli cells containing the recombinant DNA. It also relates to a method for purification of the recombinant BsmBI restriction endonuclease.

摘要翻译： 本发明涉及编码BsmBI限制性内切核酸酶以及BsmBI甲基转移酶，BsmBI限制性内切核酸酶和BsmBI甲基化酶在含有重组DNA的大肠杆菌细胞中的表达的重组DNA。 它还涉及重组BsmBI限制性内切核酸酶的纯化方法。

公开(公告)号：US20130047450A1

公开(公告)日：2013-02-28

申请号：US13528661

申请日：2012-06-20

申请人： John Pelletier

发明人： John Pelletier

IPC分类号： G01C5/00

CPC分类号： G01C15/105

摘要： A tool for projecting a light beam along a vertical surface for the purpose of determining any deviation of that surface from the true vertical, but wherein the light beam is spaced horizontally away from the vertical surface to avoid interference from projections formed in the vertical surface. The tool comprises a light projecting component and a separate, hand held target component upon which projected light impinges. The light projecting component may comprise a linear measurement scale enabling the user to determine the displacement distance of the light projector from the vertical surface. The target component comprises a corresponding linear measurement scale. When light is emitted vertically and strikes the target, measurement readings from both measurement scales are read to determine any discrepancy of the top of the vertical surface from vertical registry with a lower point along the vertical surface at which the light projector is located.

摘要翻译： 用于沿垂直表面投射光束的工具，用于确定该表面与真实垂直线的任何偏差，但是其中光束与垂直表面水平隔开，以避免在垂直表面中形成的突起的干涉。 该工具包括投光部件和分开的手持目标部件，投影光在该目标部件上撞击。 光投射部件可以包括线性测量标尺，使得用户能够确定光投射器与垂直表面的位移距离。 目标分量包括相应的线性测量标尺。 当光垂直发射并撞击目标时，读取来自两个测量尺度的测量读数，以确定垂直表面的顶部与垂直注册表之间的差异，以及沿着垂直表面放置的投影仪所在的较低点。