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公开(公告)号:US06452067B1
公开(公告)日:2002-09-17
申请号:US09156210
申请日:1998-09-17
申请人: John R. Bedbrook , Neal Gutterson , Paul W. Oeller
发明人: John R. Bedbrook , Neal Gutterson , Paul W. Oeller
IPC分类号: C12N1582
CPC分类号: C12N15/8218
摘要: This invention provides methods for identifying plant cells that exhibit post-transcriptional gene silencing (PTGS) of a chosen gene. The methods involve the use of suppression-sensitive reporter genes that, when introduced into plant cells, are expressed at a lower level in cells that exhibit PTGS than in cells that are not silenced for the particular gene.
摘要翻译: 本发明提供鉴定显示所选基因的转录后基因沉默(PTGS)的植物细胞的方法。 所述方法涉及使用抑制敏感的报道基因,当引入植物细胞时,在表现出PTGS的细胞中,比在特定基因未沉默的细胞中低表达。