Methods to assay for post-transcriptional suppression of gene expression
    5.
    发明授权
    Methods to assay for post-transcriptional suppression of gene expression 失效
    测定基因表达转录后抑制的方法

    公开(公告)号:US06452067B1

    公开(公告)日:2002-09-17

    申请号:US09156210

    申请日:1998-09-17

    IPC分类号: C12N1582

    CPC分类号: C12N15/8218

    摘要: This invention provides methods for identifying plant cells that exhibit post-transcriptional gene silencing (PTGS) of a chosen gene. The methods involve the use of suppression-sensitive reporter genes that, when introduced into plant cells, are expressed at a lower level in cells that exhibit PTGS than in cells that are not silenced for the particular gene.

    摘要翻译: 本发明提供鉴定显示所选基因的转录后基因沉默(PTGS)的植物细胞的方法。 所述方法涉及使用抑制敏感的报道基因,当引入植物细胞时,在表现出PTGS的细胞中,比在特定基因未沉默的细胞中低表达。