公开(公告)号：US06534312B1

公开(公告)日：2003-03-18

申请号：US09340798

申请日：1999-06-28

申请人： John W. Shiver ,  Mary Ellen Davies ,  Daniel C. Freed ,  Margaret A. Liu ,  Helen C. Perry

发明人： John W. Shiver ,  Mary Ellen Davies ,  Daniel C. Freed ,  Margaret A. Liu ,  Helen C. Perry

IPC分类号： C12N506

CPC分类号： C07K14/005 ,  A61K2039/51 ,  C07K2319/00 ,  C12N15/67 ,  C12N2740/16122

摘要： Synthetic polynucleotides comprising a DNA sequence encoding a peptide or protein are provided. The DNA sequence of the synthetic polynucleotides comprise codons optimized for expression in a nonhomologous host. The invention is exemplified by synthetic DNA molecules encoding HIV env as well as modifications of HIV env. The codons of the synthetic molecules include the projected host cell's preferred codons. The synthetic molecules provide preferred forms of foreign genetic material. The synthetic molecules may be used as a polynucleotide vaccine which provides immunoprophylaxis against HIV infection through neutralizing antibody and cell-mediated immunity. This invention provides polynucleotides which, when directly introduced into a vertebrate in vivo, including mammals such as primates and humans, induces the expression of encoded proteins within the animal.

公开(公告)号：US06696291B2

公开(公告)日：2004-02-24

申请号：US09974702

申请日：2001-10-09

申请人： John W. Shiver ,  Daniel C. Freed ,  Mary Ellen Davies ,  Margaret A. Liu ,  Helen C. Perry

发明人： John W. Shiver ,  Daniel C. Freed ,  Mary Ellen Davies ,  Margaret A. Liu ,  Helen C. Perry

IPC分类号： C12N506

CPC分类号： C07K14/005 ,  A61K39/00 ,  C07K2319/02 ,  C12N2740/16222 ,  Y02A50/412

摘要： Synthetic DNA molecules encoding HIV gag and modifications of HIV gag are provided. The codons of the synthetic molecules are codons preferred by the projected host cell. The synthetic molecules may be used as a polynucleotide vaccine which provides effective immunoprophylaxis against HIV infection through stimulation of neutralizing antibody and cell-mediated immunity.