公开(公告)号：US6074835A

公开(公告)日：2000-06-13

申请号：US837058

申请日：1997-04-11

申请人： Johnathan Braun ,  Stephan R. Targan ,  Mark Eggena

发明人： Johnathan Braun ,  Stephan R. Targan ,  Mark Eggena

IPC分类号： G01N33/68 ,  G01N33/564

CPC分类号： G01N33/6875 ,  G01N2800/065

摘要： The present invention provides a method of diagnosing ulcerative colitis (UC) in a subject suspected of having inflammatory bowel disease by by obtaining a sample from the individual; contacting the sample with human histone H1, or pANCA-reactive fragment thereof, under conditions suitable to form a complex of human histone H1, or pANCA-reactive fragment thereof, and antibody to human histone H1; and detecting the presence or absence of the complex, where the presence of the complex indicates that the individual has UC. The invention also provides related methods of diagnosing a perinuclear anti-neutrophil cytoplasmic antibody positive (pANCA-positive) clinical subtype of UC in a patient with UC. In addition, the invention further provides methods of determining susceptibility to UC in an individual. The invention further provides methods of inducing tolerance in a pANCA-positive patient with UC by administering an effective dose of histone H1, or tolerogenic fragment thereof, to the pANCA-positive patient with UC. Compositions of histone H1, or fragment thereof, combined with a tolerogizing molecule also are provided.

摘要翻译： 本发明提供一种通过从个体获得样品来诊断疑似患有炎症性肠病的受试者的溃疡性结肠炎（UC）的方法; 在适于形成人组蛋白H1或其pANCA反应性片段的复合物和人组蛋白H1的抗体的条件下，将样品与人组蛋白H1或其pANCA反应性片段接触; 并检测复合物的存在或不存在，其中复合物的存在表明个体具有UC。 本发明还提供了在UC患者中诊断UC的核周抗中性粒细胞胞质抗体阳性（pANCA阳性）临床亚型的相关方法。 此外，本发明还提供了确定个体对UC的易感性的方法。 本发明进一步提供了通过向具有UC的pANCA阳性患者施用有效剂量的组蛋白H1或其耐受性片段来诱导具有UC的pANCA阳性患者的耐受性的方法。 还提供组蛋白H1或其片段的组合物与耐受性分子组合。