公开(公告)号：US20110287534A1

公开(公告)日：2011-11-24

申请号：US13099963

申请日：2011-05-03

申请人： Jonathan Rowley ,  Patrick Newsom

发明人： Jonathan Rowley ,  Patrick Newsom

IPC分类号： C12N5/071 ,  C12M3/00

CPC分类号： A01N1/0268

摘要： An apparatus and processes for aseptically dispensing live mammalian cells into sterile, flexible bags in a non-sterile atmosphere. The method includes the steps of: providing the cells suspended in a liquid; providing a plurality of sterile flexible bags fluidly connected to a main line by a plurality of branch lines of sterile flexible tubing; evacuating air from the flexible bags by applying a vacuum to the open end of the main line; preventing fluid flow through all branch lines except that of one bag to be filled; dispensing a desired volume of cell suspension into the open end of the main line; and introducing sufficient sterile purging gas under pressure into open end of the main line to drive into the bag any of the dispensed volume remaining in the main line or branch line of filled bag. Cells can be cryogenically preserved in the filled bags.

摘要翻译： 用于在非无菌气氛中将活的哺乳动物细胞无菌分配到无菌的柔性袋中的装置和方法。 该方法包括以下步骤：提供悬浮在液体中的细胞; 提供多个无菌柔性袋，其通过多条无菌柔性管的分支管线流体地连接到主线; 通过向主线的开口端施加真空从空气中排出空气; 防止流体通过所有分支管线，除了一个待填充的袋子; 将所需体积的细胞悬浮液分配到主线的开口端; 并将足够的无菌吹扫气体在压力下引入主管线的开口端以驱动残留在填充袋的主线或分支管线中的任何分配体积。 细胞可以被低温保存在填充的袋子中。