公开(公告)号：US06810713B2

公开(公告)日：2004-11-02

申请号：US10195320

申请日：2002-07-16

申请人： Jong Hoon Hahn ,  Kwanscop Lim ,  Kihoon Na ,  Suhyeon Kim ,  Je-Kyun Park

发明人： Jong Hoon Hahn ,  Kwanscop Lim ,  Kihoon Na ,  Suhyeon Kim ,  Je-Kyun Park

IPC分类号： G01N100

CPC分类号： G01N30/32 ,  F04B43/043 ,  F04B43/12 ,  G01N2030/326 ,  Y10T436/2575

摘要： Various kinds of fluid can be handled and delivered accurately in infinitesimal amounts through microchannels formed in an elastic polymeric substrate by periodically squeezing selective portions of the microchannel using a rotor to apply an external force on the elastic substrate. The delivery rate of the fluid is in the range from pL/sec to mL/sec determined by the rotation speed of the rotor which presses the microchannels and the dimensions of the microchannel. Regardless of physical properties of the fluid to be delivered, various kinds of fluids can be accurately delivered through the microchannels in the desired amount.

摘要翻译： 可以通过使用转子周期性地挤压微通道的选择性部分来在弹性基底上施加外力，可以以无限量的量以极小的量精确地处理和传送各种流体，通过形成在弹性聚合物基底中的微通道。 流体的输送速率在由按压微通道的转子的旋转速度和微通道的尺寸确定的pL / sec至mL / sec的范围内。 不管要输送的流体的物理性质如何，可以通过微通道以期望的量准确地输送各种流体。

公开(公告)号：US20080192254A1

公开(公告)日：2008-08-14

申请号：US11872384

申请日：2007-10-15

申请人： Suhyeon Kim ,  Jintae Kim

发明人： Suhyeon Kim ,  Jintae Kim

IPC分类号： G01N21/64

CPC分类号： G01N21/645

摘要： A system and a method for detecting fluorescence in a microfluidic chip having a microchamber and measuring the amount of the fluorescence are disclosed. In the system and method, an optical device detects fluorescence generated by a fluorescent material. The position of the outer circumference of a microfluidic chip is identified based on the change of the amount of the detected fluorescence. Then, the position of a microchannel is calculated based on the position of the outer circumference of the microfluidic chip and the distance between the outer circumference and the microchamber. Hence, the amount of the fluorescence only generated from the microchamber of the microfluidic chip is automatically calculated. Further, a plurality of samples can be analyzed at the same time, thereby reducing analysis time and cost.

摘要翻译： 公开了一种用于检测具有微室并且测量荧光量的微流体芯片中的荧光的系统和方法。 在该系统和方法中，光学装置检测由荧光材料产生的荧光。 基于检测到的荧光的量的变化来识别微流体芯片的外周的位置。 然后，基于微流体芯片的外周的位置和外周与微室之间的距离来计算微通道的位置。 因此，仅从微流控芯片的微室产生的荧光量被自动计算。 此外，可以同时分析多个样本，从而减少分析时间和成本。

公开(公告)号：US20080297792A1

公开(公告)日：2008-12-04

申请号：US11972896

申请日：2008-01-11

申请人： Suhyeon Kim ,  Jin-tae Kim

发明人： Suhyeon Kim ,  Jin-tae Kim

IPC分类号： G01N21/64

CPC分类号： G01N21/645 ,  B01L3/502715 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/4406 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2201/062

摘要： A fluorescence detecting module for detecting fluorescence in a microchamber and a fluorescence detecting system. The fluorescence detecting module includes a light source irradiating excitation light a collimating lens condensing excitation light irradiated, a dichroic mirror selectively transmitting or reflecting the light according to a wavelength thereof, an objective lens condensing excitation light selected to be irradiated on a sample in a microchamber and condensing fluorescence generated in the microchamber, a focusing lens focusing fluorescence selected by the dichroic mirror, and a fluorescence detecting element detecting fluorescence focused. The fluorescence detecting system for a microfluid chip in which microchambers are arranged, includes a frame, at least one fluorescence detecting module, a holder supporting the fluorescence detecting module, a driver allowing the holder to make a reciprocating motion along a direction in which the microchambers are arranged, and a guide supporting the holder to be moved and guiding the movement.

摘要翻译： 用于检测微室中的荧光的荧光检测模块和荧光检测系统。 荧光检测模块包括照射激发光的光源，照射的聚光激发光的准直透镜，根据其波长选择性地透射或反射光的二向色镜，将被选择照射在微型室中的样品上的激发光聚焦的物镜 并且聚焦在微室中产生的荧光，聚焦透镜，聚焦由分色镜选择的荧光，以及检测荧光的荧光检测元件。 微流体芯片布置的微流体芯片的荧光检测系统包括框架，至少一个荧光检测模块，支撑荧光检测模块的保持器，允许保持器沿微孔室方向往复运动的驱动器 以及支撑待移动的保持器并引导运动的引导件。

公开(公告)号：US07859670B2

公开(公告)日：2010-12-28

申请号：US11872384

申请日：2007-10-15

申请人： Suhyeon Kim ,  Jintae Kim

发明人： Suhyeon Kim ,  Jintae Kim

IPC分类号： G01N21/25

CPC分类号： G01N21/645

摘要： A system and a method for detecting fluorescence in a microfluidic chip having a microchamber and measuring the amount of the fluorescence are disclosed. In the system and method, an optical device detects fluorescence generated by a fluorescent material. The position of the outer circumference of a microfluidic chip is identified based on the change of the amount of the detected fluorescence. Then, the position of a microchannel is calculated based on the position of the outer circumference of the microfluidic chip and the distance between the outer circumference and the microchamber. Hence, the amount of the fluorescence only generated from the microchamber of the microfluidic chip is automatically calculated. Further, a plurality of samples can be analyzed at the same time, thereby reducing analysis time and cost.

摘要翻译： 公开了一种用于检测具有微室并且测量荧光量的微流体芯片中的荧光的系统和方法。 在该系统和方法中，光学装置检测由荧光材料产生的荧光。 基于检测到的荧光的量的变化来识别微流体芯片的外周的位置。 然后，基于微流体芯片的外周的位置和外周与微室之间的距离来计算微通道的位置。 因此，仅从微流控芯片的微室产生的荧光量被自动计算。 此外，可以同时分析多个样本，从而减少分析时间和成本。

公开(公告)号：US20090139578A1

公开(公告)日：2009-06-04

申请号：US12182545

申请日：2008-07-30

申请人： Suhyeon Kim ,  Jeonggun Lee ,  Beom-seok Lee

发明人： Suhyeon Kim ,  Jeonggun Lee ,  Beom-seok Lee

IPC分类号： F16K17/36

CPC分类号： B01L3/502715 ,  B01L3/502753 ,  B01L2200/025 ,  B01L2300/02 ,  B01L2300/0627 ,  B01L2300/0806 ,  B01L2300/168 ,  B01L2400/0409 ,  Y10T137/0391 ,  Y10T137/0753

摘要： Provided are a centrifugal force based platform formed to be rotatable and including a home mark having a retro-reflective property of light, and a centrifugal force based microfluidic system including the platform. The method of determining a home position of the centrifugal force based platform includes: rotating the platform formed and including a home mark having a retro-reflective property of light; emitting light from a light-emitting unit to the platform; and detecting the emitted light, which is retro-reflected by the home mark, in a light-receiving unit, and then determining the home position of the platform based on the detected light.

摘要翻译： 提供了一种基于离心力的平台，其形成为可旋转的，并且包括具有光的反射性能的归位标记，以及包括该平台的基于离心力的微流体系统。 确定基于离心力的平台的起始位置的方法包括：旋转所形成的平台并且包括具有光的回射性能的原点标记; 将发光单元发射到平台; 并且在光接收单元中检测由归属标记反射的发射光，然后基于检测到的光来确定平台的原始位置。

公开(公告)号：US08242433B2

公开(公告)日：2012-08-14

申请号：US12182545

申请日：2008-07-30

申请人： Suhyeon Kim ,  Jeonggun Lee ,  Beom-seok Lee

发明人： Suhyeon Kim ,  Jeonggun Lee ,  Beom-seok Lee

IPC分类号： G01D5/34

CPC分类号： B01L3/502715 ,  B01L3/502753 ,  B01L2200/025 ,  B01L2300/02 ,  B01L2300/0627 ,  B01L2300/0806 ,  B01L2300/168 ,  B01L2400/0409 ,  Y10T137/0391 ,  Y10T137/0753

摘要： Provided are a centrifugal force based platform formed to be rotatable and including a home mark having a retro-reflective property of light, and a centrifugal force based microfluidic system including the platform. The method of determining a home position of the centrifugal force based platform includes: rotating the platform formed and including a home mark having a retro-reflective property of light; emitting light from a light-emitting unit to the platform; and detecting the emitted light, which is retro-reflected by the home mark, in a light-receiving unit, and then determining the home position of the platform based on the detected light.

摘要翻译： 提供了一种基于离心力的平台，其形成为可旋转的，并且包括具有光的反射性能的归位标记，以及包括该平台的基于离心力的微流体系统。 确定基于离心力的平台的起始位置的方法包括：旋转所形成的平台并且包括具有光的回射性能的原点标记; 将发光单元发射到平台; 并且在光接收单元中检测由归属标记反射的发射光，然后基于检测到的光来确定平台的原始位置。