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    Method for isolating a nucleic acid using particulate matter and a composition therefor
    1.
    发明授权
    Method for isolating a nucleic acid using particulate matter and a composition therefor 有权
    使用颗粒物分离核酸的方法及其组合物

    公开(公告)号：US08530639B2

    公开(公告)日：2013-09-10

    申请号：US12448134

    申请日：2007-12-11

    申请人： Jong-Hoon Kim ,  Min Kim ,  Hae-Joon Park ,  Han Oh Park

    发明人： Jong-Hoon Kim ,  Min Kim ,  Hae-Joon Park ,  Han Oh Park

    IPC分类号： C07H21/00

    CPC分类号： C07H21/00 ,  C12N15/1003

    摘要： A method for isolating a nucleic acid from a biological sample includes applying particulate matter to promote co-aggregation and co-precipitation of insoluble aggregate by directly adding to the biological sample, adding to the biological sample in admixture with a cell lysis buffer, adding to the biological sample treated with a cell lysis buffer, adding to cell lysates in admixture with a buffer for forming denatured protein aggregate; or adding to cell lysates comprising the formed denatured protein aggregate. The particulate matter is selected from the group consisting of a material formed from an element of Ag, Fe, Ti, Al, Sn, Si, Cu, Mo, Ni, W or Zn, an oxide, a carbide, a nitride, a boride and a silicide thereof, and a mixture thereof, a polymer selected from PMMA (Poly Methyl MethAcrylate), polyethylene or polyurethane; and a mixture thereof. The insoluble aggregate comprises denatured protein aggregate and cell debris.

    摘要翻译： 从生物样品中分离核酸的方法包括通过直接添加到生物样品中来加入颗粒物质以促进不溶性聚集体的共聚和共沉淀，将生物样品与细胞裂解缓冲液混合，加入到 用细胞裂解缓冲液处理生物样品，加入细胞裂解物与缓冲液混合以形成变性蛋白质聚集体; 或加入包含形成的变性蛋白质聚集体的细胞裂解物。 颗粒物质选自由Ag，Fe，Ti，Al，Sn，Si，Cu，Mo，Ni，W或Zn的元素形成的材料，氧化物，碳化物，氮化物，硼化物 及其硅化物及其混合物，选自PMMA（聚甲基丙烯酸甲酯），聚乙烯或聚氨酯的聚合物; 及其混合物。 不溶性聚集体包含变性蛋白质聚集体和细胞碎片。

    METHOD FOR ISOLATING A NUCLEIC ACID USING PARTICULATE MATTER AND A COMPOSITION THEREFOR
    2.
    发明申请
    METHOD FOR ISOLATING A NUCLEIC ACID USING PARTICULATE MATTER AND A COMPOSITION THEREFOR 有权
    使用颗粒物分解核酸的方法及其组合物

    公开(公告)号：US20100197903A1

    公开(公告)日：2010-08-05

    申请号：US12448134

    申请日：2007-12-11

    申请人： Jong-Hoon Kim ,  Min Kim ,  Hae-Joon Park ,  Han Oh Park

    发明人： Jong-Hoon Kim ,  Min Kim ,  Hae-Joon Park ,  Han Oh Park

    IPC分类号： C07H21/00 ,  B01J20/26 ,  C09K3/00

    CPC分类号： C07H21/00 ,  C12N15/1003

    摘要： Disclosed are a method for isolating a nucleic acid using particulate matter and a composition therefor. The method comprises essentially the steps of adding a lysis buffer and a neutralization buffer to a biological sample sequentially, and centrifuging cell lysates for at least 10 minutes to separate a solution comprising the nucleic acid and insoluble aggregate comprising denatured protein aggregate and cell debris. The particulate matter is added to and participates in co-aggregation and co-precipitation of denatured protein aggregate and cell debris, and co-aggregates and co-precipitates denatured protein aggregate and cell debris within a much shortened time, thereby to shorten the time and to increase the yield for the isolation of a nucleic acid, compared with conventional methods.

    摘要翻译： 公开了使用颗粒物质及其组合物分离核酸的方法。 该方法基本上包括以下步骤：依次向生物样品中加入裂解缓冲液和中和缓冲液，并离心细胞裂解物至少10分钟以分离包含核酸的溶液和包含变性蛋白质聚集体和细胞碎片的不溶性骨架。 添加颗粒物并参与变性蛋白质聚集体和细胞碎片的共聚和共沉淀，并在更短的时间内共同聚集和共沉淀变性蛋白质聚集体和细胞碎片，从而缩短时间和 以提高分离核酸的产量，与常规方法相比。

    Sponge holder
    7.
    外观设计
    Sponge holder 有权

    公开(公告)号：USD903370S1

    公开(公告)日：2020-12-01

    申请号：US29686718

    申请日：2019-04-06

    申请人： Min Kim

    设计人： Min Kim

    Billing device and processing method
    8.
    发明授权
    Billing device and processing method 有权
    计费装置和处理方法

    公开(公告)号：US08756126B2

    公开(公告)日：2014-06-17

    申请号：US12538037

    申请日：2009-08-07

    申请人： Byoung-Cheol Choi ,  Jae-Sung Kim ,  Seog-Cheon Jeon ,  Min Kim ,  Yoo-Hoon Won

    发明人： Byoung-Cheol Choi ,  Jae-Sung Kim ,  Seog-Cheon Jeon ,  Min Kim ,  Yoo-Hoon Won

    IPC分类号： G06Q20/14 ,  G06Q10/10 ,  G06Q30/04

    CPC分类号： G06Q30/04 ,  G06Q10/107 ,  G06Q20/325

    摘要： A billing device and processing method is disclosed. In one embodiment, the method includes i) collecting a billing log according to performing a biz template at the business logic processing unit, ii) verifying validity of the collected billing log, iii) performing a standardizing process on the verified billing log, iv) generating billing data by applying a predetermined billing policy according to the standardized billing log, v) generating a billing file (MDR) to transmit to a billing legacy system of a mobile communication system using the generated billing data and vi) transmitting the generated billing file (MDR) to the billing legacy system.

    摘要翻译： 公开了计费装置和处理方法。 在一个实施例中，该方法包括：i）根据业务逻辑处理单元执行biz模板收集记帐日志; ii）验证所收集的记帐日志的有效性; iii）对已验证的记账日志执行标准化处理; iv） 通过根据标准化记账日志应用预定的计费政策来生成计费数据，v）生成使用生成的计费数据向移动通信系统的计费遗留系统发送的计费文件（MDR），以及vi）发送生成的计费文件 （MDR）到计费遗留系统。

    Open mobile business supporting system and method
    9.
    发明授权
    Open mobile business supporting system and method 有权
    开放移动业务支撑系统和方法

    公开(公告)号：US08230040B2

    公开(公告)日：2012-07-24

    申请号：US12114690

    申请日：2008-05-02

    申请人： Byoung-Cheol Choi ,  Jae-Sung Kim ,  Seog-Cheon Jeon ,  Min Kim ,  Yoo-Hoon Won

    发明人： Byoung-Cheol Choi ,  Jae-Sung Kim ,  Seog-Cheon Jeon ,  Min Kim ,  Yoo-Hoon Won

    IPC分类号： G06F15/16

    CPC分类号： G06Q30/04 ,  G06Q10/107 ,  G06Q20/325

    摘要： An open mobile business supporting method and system are disclosed for effectively supporting various business models desired by a contents/service provider by providing single interface that can integrally use various resources of a mobile communication system. Low-level functions, which can be grouped as a unit function and performed in a mobile communication system, are selected among service logics of a CP/SP and defined as biz logic. The biz logic is performed according to a request of CP/SP by cooperating with a mobile communication system and various legacy systems. The result of performing the biz logic is returned to the CP/SP.

    摘要翻译： 公开了一种开放的移动业务支持方法和系统，用于通过提供可以整体地使用移动通信系统的各种资源的单个接口来有效地支持内容/服务提供商所期望的各种业务模型。 可以分组为单元功能并在移动通信系统中执行的低级功能在CP / SP的服务逻辑中选择并定义为biz逻辑。 根据CP / SP的请求，通过与移动通信系统和各种传统系统协作来执行biz逻辑。 执行biz逻辑的结果返回给CP / SP。

    Business logic device and processing method
    10.
    发明授权
    Business logic device and processing method 有权
    业务逻辑器件和处理方法

    公开(公告)号：US08073932B2

    公开(公告)日：2011-12-06

    申请号：US12537940

    申请日：2009-08-07

    申请人： Byoung-Cheol Choi ,  Jae-Sung Kim ,  Seog-Cheon Jeon ,  Min Kim ,  Yoo-Hoon Won

    发明人： Byoung-Cheol Choi ,  Jae-Sung Kim ,  Seog-Cheon Jeon ,  Min Kim ,  Yoo-Hoon Won

    IPC分类号： G06F15/16

    CPC分类号： G06Q30/04 ,  G06Q10/107 ,  G06Q20/325

    摘要： A business logic device and processing method is disclosed. In one embodiment, the method includes i) receiving a request message for performing a biz template from the open interface processing unit, ii) analyzing the received biz template performing request message and performing the biz template by cooperating with a legacy cooperation processing unit and iii) composing a message for a result of performing the stored biz template and transmitting the composed message to the open interface processing unit.

    摘要翻译： 公开了业务逻辑设备和处理方法。 在一个实施例中，该方法包括：i）从开放接口处理单元接收用于执行biz模板的请求消息，ii）分析所接收的biz模板执行请求消息并通过与遗留协作处理单元和iii协作来执行biz模板 ）构成用于执行所存储的biz模板并将合成消息发送到开放接口处理单元的结果的消息。