公开(公告)号：US20080070853A1

公开(公告)日：2008-03-20

申请号：US10549048

申请日：2004-01-13

申请人： Josef Michl ,  Jesko Koehnke ,  Matthew R. Pincus

发明人： Josef Michl ,  Jesko Koehnke ,  Matthew R. Pincus

IPC分类号： A61K48/00 ,  C12N15/63

CPC分类号： C07K14/4746 ,  A61K38/00

摘要： The present invention provides peptides corresponding to all or a portion of amino acid residues 12-26 of human p53 protein, which peptides are lethal to malignant or transformed cells when fused to a membrane-penetrating leader sequence. The subject peptides are thus useful in treating neoplastic disease in an animal, preferably a human. Also provided are pharmaceutical compositions comprising the subject peptides admixed with a pharmaceutical acceptable carrier. Methods of treating neoplastic disease in a patient by administering a subject peptide fused at its carboxy terminal end to a membrane penetrating leader sequence are also provided. The present invention also provides replication incompetent Adenovirus (AdV) vectors comprising a promoter sequence operably linked to a nucleotide sequence encoding a subject peptide. Methods of selectively killing cancer cells in a subject by administering a therapeutically effective amount of a subject AdV vector are also provided by the present invention.

摘要翻译： 本发明提供对应于人p53蛋白的全部或部分氨基酸残基12-26的肽，当与透膜前导序列融合时，该肽对恶性或转化细胞致死。 因此，本发明的肽可用于治疗动物，优选人类的肿瘤性疾病。 还提供了包含与药学上可接受的载体混合的受试肽的药物组合物。 还提供了通过将其羧基末端融合的受试肽施用于穿透前导序列的方法来治疗患者的肿瘤疾病。 本发明还提供了复制无能力的腺病毒（Adv）载体，其包含可操作地连接到编码目标肽的核苷酸序列的启动子序列。 通过施用治疗有效量的受试者AdV载体选择性地杀死受试者的癌细胞的方法也由本发明提供。

公开(公告)号：US07883888B2

公开(公告)日：2011-02-08

申请号：US10549048

申请日：2004-01-13

申请人： Josef Michl ,  Jesko Koehnke ,  Matthew R. Pincus

发明人： Josef Michl ,  Jesko Koehnke ,  Matthew R. Pincus

IPC分类号： C12N15/00

CPC分类号： C07K14/4746 ,  A61K38/00

摘要： The present invention provides peptides corresponding to all or a portion of amino acid residues 12-26 of human p53 protein, which peptides are lethal to malignant or transformed cells when fused to a membrane-penetrating leader sequence. The subject peptides are thus useful in treating neoplastic disease in an animal, preferably a human. Also provided are pharmaceutical compositions comprising the subject peptides admixed with a pharmaceutical acceptable carrier. Methods of treating neoplastic disease in a patient by administering a subject peptide fused at its carboxy terminal end to a membrane penetrating leader sequence are also provided. The present invention also provides replication incompetent Adenovirus (AdV) vectors comprising a promoter sequence operably linked to a nucleotide sequence encoding a subject peptide. Methods of selectively killing cancer cells in a subject by administering a therapeutically effective amount of a subject AdV vector are also provided by the present invention.

摘要翻译： 本发明提供对应于人p53蛋白的全部或部分氨基酸残基12-26的肽，当与透膜前导序列融合时，该肽对恶性或转化细胞致死。 因此，本发明的肽可用于治疗动物，优选人类的肿瘤性疾病。 还提供了包含与药学上可接受的载体混合的受试肽的药物组合物。 还提供了通过将其羧基末端融合的受试肽施用于穿透前导序列的方法来治疗患者的肿瘤疾病。 本发明还提供了复制无能力的腺病毒（Adv）载体，其包含可操作地连接到编码目标肽的核苷酸序列的启动子序列。 通过施用治疗有效量的受试者AdV载体选择性地杀死受试者的癌细胞的方法也由本发明提供。

公开(公告)号：US20110124011A1

公开(公告)日：2011-05-26

申请号：US13054658

申请日：2009-08-04

申请人： Rainer Niedenthal ,  Astrid Jakobs ,  Jesko Koehnke

发明人： Rainer Niedenthal ,  Astrid Jakobs ,  Jesko Koehnke

IPC分类号： G01N33/53 ,  C12N15/63

CPC分类号： C12Q1/25

摘要： The present invention relates to a method for determining SUMOylation and utilizing said SUMOylation patterns for identifying specific interaction between different binding partners. In another aspect, the present invention relates to systems allowing the determination of SUMOylation and for determining specific interaction between binding partners. Furthermore, the present invention relates to vectors and proteins relating to SUMOylation.

摘要翻译： 本发明涉及一种用于确定SUMO化并利用所述SUMO化模式用于鉴定不同结合配偶体之间的特异性相互作用的方法。 在另一方面，本发明涉及允许确定SUMO化和确定结合配偶体之间的特异性相互作用的系统。 此外，本发明涉及与SUMO化有关的载体和蛋白质。

公开(公告)号：US08551717B2

公开(公告)日：2013-10-08

申请号：US13054658

申请日：2009-08-04

申请人： Rainer Niedenthal ,  Astrid Jakobs ,  Jesko Koehnke

发明人： Rainer Niedenthal ,  Astrid Jakobs ,  Jesko Koehnke

IPC分类号： G01N31/00 ,  G01N33/53

CPC分类号： C12Q1/25

摘要： The present invention relates to a method for determining SUMOylation and utilizing said SUMOylation patterns for identifying specific interaction between different binding partners. In another aspect, the present invention relates to systems allowing the determination of SUMOylation and for determining specific interaction between binding partners. Furthermore, the present invention relates to vectors and proteins relating to SUMOylation.

摘要翻译： 本发明涉及一种用于确定SUMO化并利用所述SUMO化模式用于鉴定不同结合配偶体之间的特异性相互作用的方法。 在另一方面，本发明涉及允许确定SUMO化和确定结合配偶体之间的特异性相互作用的系统。 此外，本发明涉及与SUMO化有关的载体和蛋白质。