Thermostable DNA polymerases and methods of making same

    公开(公告)号:US20060035360A1

    公开(公告)日:2006-02-16

    申请号:US11242730

    申请日:2005-10-04

    申请人: Joseph Farchaus

    发明人: Joseph Farchaus

    CPC分类号: C12N9/1252

    摘要: The present invention relates to methods and compositions for providing purified thermostable enzymes, particularly thermostable DNA polymerases, that are free of exogenous detergents. The present invention also provides methods for providing such purified thermostable DNA polymerases to assays in an active form by adding one or more detergents. The present invention further provides compositions and kits comprising purified thermostable DNA polymerases for use in a variety of applications, including amplification and sequencing of nucleic acids.