公开(公告)号：US20120109533A1

公开(公告)日：2012-05-03

申请号：US13309038

申请日：2011-12-01

申请人： Joseph KWON ,  Chiyoul PARK ,  Gookpil ROH ,  Taehoon LEE ,  Jongsoon CHOI

发明人： Joseph KWON ,  Chiyoul PARK ,  Gookpil ROH ,  Taehoon LEE ,  Jongsoon CHOI

IPC分类号： G06F19/00

CPC分类号： H01J49/0036 ,  G01N33/6848 ,  G01N2030/8831 ,  H01J49/0031

摘要： The present invention relates to a method of analyzing a protein or proteins comprising the steps of: (A) pre-treating a mixture containing at least one protein to obtain peptides; (B) obtaining information about retention times and mass values of the obtained peptides by performing data-independent analysis; (C) searching a first database on the basis of the information obtained in step (B) to quantify and qualify a target protein or proteins; (D) extracting information about the quantified and qualified target protein or proteins; (E) obtaining information about retention times and mass values by performing data-dependent analysis from the extracted information of step (D); (F) searching a second database on the basis of the information obtained in step (E) to further quantify and qualify the target protein or proteins; and (G) comparing the search results of step (C) and (F) to verify the quantification and qualification.

摘要翻译： 本发明涉及分析蛋白质或蛋白质的方法，包括以下步骤：（A）预处理含有至少一种蛋白质的混合物以获得肽; （B）通过进行数据无关分析获得获得的肽的保留时间和质量值的信息; （C）基于在步骤（B）中获得的信息来搜索第一数据库以定量和鉴定靶蛋白质; （D）提取关于定量和合格的靶蛋白或蛋白质的信息; （E）通过从所提取的步骤（D）的信息执行数据相关分析，获得关于保留时间和质量值的信息; （F）基于在步骤（E）中获得的信息来搜索第二数据库以进一步量化和鉴定目标蛋白质或蛋白质; 和（G）比较步骤（C）和（F）的搜索结果以验证量化和鉴定。