公开(公告)号：US20110091930A1

公开(公告)日：2011-04-21

申请号：US12866970

申请日：2009-02-13

申请人： Joseph P. Vacanti ,  Howard I. Pryor ,  Craig M. Neville ,  Ira Spool

发明人： Joseph P. Vacanti ,  Howard I. Pryor ,  Craig M. Neville ,  Ira Spool

IPC分类号： C12Q1/20 ,  C12M3/00 ,  C12N5/071

CPC分类号： G01N33/5014 ,  C12M25/04 ,  C12M35/08 ,  G01N33/5067

摘要： A well-based flow system for cell culture is described which provides for flow of culture containing compounds for drug screening to be exposed to cells seeded on a membrane. The flow of medium may be planar or radial and means are provided for the removal of waste media through fluid outlets in fluid communication with the assay well plates through conduits. Methods of using the system for cell culture and drug toxicity screening are also provided including coculturing cells such as hepatocytes, stem cells, fibroblasts and smooth muscle cells and selectively exposing cells to test compounds.

摘要翻译： 描述了用于细胞培养的基于基础的流动系统，其提供含有用于药物筛选的化合物的培养物流，以暴露于接种在膜上的细胞。 介质的流动可以是平面的或径向的，并且提供了用于通过导管与测定孔板流体连通的流体出口去除废弃介质的装置。 还提供了使用该系统用于细胞培养和药物毒性筛选的方法，包括共培养细胞如肝细胞，干细胞，成纤维细胞和平滑肌细胞，并选择性地将细胞暴露于测试化合物。