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1.发明申请Artificial protein, method for absolute quantification of proteins and uses thereof 审中-公开人造蛋白，蛋白质的绝对定量方法及其用途公开(公告)号：US20090137050A1
公开(公告)日：2009-05-28
申请号：US11916292
申请日：2005-11-18
申请人： Julie M. Pratt , Robert Beynon , Simon Gaskell
发明人： Julie M. Pratt , Robert Beynon , Simon Gaskell
摘要： The invention provides an artificial protein for quantitative analysis of the proteome of a sample, cell or organism, comprising at least two consecutive peptides linked by a cleavage sequence for separating the peptides; a singular marker on one or more peptide for determination of the absolute amount of this fragment; and N-terminal and C-terminal extensions for protection of the peptides; wherein each peptide represents one single protein of the sample, cell or organism and each peptide is in a defined stoichiometry. The invention further provides a collection of peptides, a vector and a kit comprising the artificial protein and a method for quantitative analysis of the proteome.
摘要翻译： 本发明提供用于定量分析样品，细胞或生物体的蛋白质组的人造蛋白质，其包含通过用于分离肽的切割序列连接的至少两个连续肽; 用于确定该片段绝对量的一种或多种肽上的单一标记; 和用于保护肽的N-末端和C-末端延伸; 其中每个肽表示样品，细胞或生物体的一个单一蛋白质，并且每个肽都在限定的化学计量学中。 本发明进一步提供肽，载体和包含人造蛋白的试剂盒和蛋白质组的定量分析方法的集合。
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公开(公告)号：US08399402B2
公开(公告)日：2013-03-19
申请号：US12599960
申请日：2008-04-15
申请人： Robert Beynon , Simon Gaskell , Julie Pratt , Claire Eyers
发明人： Robert Beynon , Simon Gaskell , Julie Pratt , Claire Eyers
IPC分类号： A61K38/00
CPC分类号： G01N33/6851 , C07K5/1008 , C07K7/06 , C07K7/08 , C07K14/001 , G01N2458/15 , G01N2496/00
摘要： The present invention provides a polypeptide as standard for peptide analysis by mass spectrometry comprising at least 16 peptides selected from the group consisting of the peptides of SEQ ID NO: 1 to SEQ ID NO: 22 or variants thereof, together with an artificial protein comprising the polypeptide, a vector comprising a nucleic acid encoding the polypeptide, a kit for proteome analysis, a selection of peptides for calibration an devaluation of mass spectrometers and chromatographs for proteome analysis and uses thereof.
摘要翻译： 本发明提供了通过质谱法进行肽分析的标准多肽，其包含至少16种选自SEQ ID NO：1至SEQ ID NO：22的肽或其变体的肽，以及包含 多肽，包含编码多肽的核酸的载体，用于蛋白质组分析的试剂盒，用于校准质谱仪的贬值的肽的选择和用于蛋白质组分析的色谱仪及其用途。
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公开(公告)号：US20100197889A1
公开(公告)日：2010-08-05
申请号：US12599960
申请日：2008-04-15
申请人： Robert Beynon , Simon Gaskell , Julie Pratt , Claire Eyers
发明人： Robert Beynon , Simon Gaskell , Julie Pratt , Claire Eyers
CPC分类号： G01N33/6851 , C07K5/1008 , C07K7/06 , C07K7/08 , C07K14/001 , G01N2458/15 , G01N2496/00
摘要： The present invention provides a polypeptide as standard for peptide analysis by mass spectrometry comprising at least 16 peptides selected from the group consisting of the peptides of SEQ ID NO: 1 to SEQ ID NO: 22 or variants thereof, together with an artificial protein comprising the polypeptide, a vector comprising a nucleic acid encoding the polypeptide, a kit for proteome analysis, a selection of peptides for calibration an devaluation of mass spectrometers and chromatographs for proteome analyses and uses thereof.
摘要翻译： 本发明提供了通过质谱法进行肽分析的标准多肽，其包含至少16种选自SEQ ID NO：1至SEQ ID NO：22的肽或其变体的肽，以及包含 多肽，包含编码多肽的核酸的载体，用于蛋白质组分析的试剂盒，用于校准质谱仪的贬值的肽的选择和用于蛋白质组分析的色谱仪及其用途。
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