公开(公告)号：US11821027B2

公开(公告)日：2023-11-21

申请号：US16476856

申请日：2018-01-10

申请人： Juno Therapeutics, Inc.

发明人： Mark L. Bonyhadi ,  David G. Kugler ,  Timothy G. Johnstone ,  Ronald James Hause, Jr. ,  Lucas J. Thompson ,  Ryan P. Larson

IPC分类号： C12Q1/6827 ,  G16B30/10 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6886 ,  G16B20/20 ,  G16B40/10 ,  G16B20/10

CPC分类号： C12Q1/6827 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6886 ,  G16B20/10 ,  G16B20/20 ,  G16B30/10 ,  G16B40/10 ,  C12Q2600/142 ,  C12Q2600/154 ,  C12Q2600/156

摘要： Provided herein are methods of identifying genomic region(s) predictive of an outcome of treatment with a cell therapy and/or of a phenotype of function of the cells. In some embodiments, the methods include epigenetic and/or epigenomic analyses of the cells in connection with methods for preparing engineered cells for cell therapy and/or predicting response to a cell therapy, e.g., engineered cells for cell therapy. In some embodiments, the methods include steps to assess, characterize and analyze changes or modifications in an epigenetic property of gene region or regions, such as chromatin accessibility, nucleosome occupancy, histone modification, spatial chromosomal conformation, transcription factor occupancy and/or DNA methylation. In some embodiments, the epigenetic and/or epigenomic analysis includes determining the epigenetic properties of a cell, e.g., an engineered cell for cell therapy.