公开(公告)号：US11796534B2

公开(公告)日：2023-10-24

申请号：US16604547

申请日：2018-04-13

申请人： Juno Therapeutics, Inc.

发明人： Paul Ken Kodama ,  Tom Kowski ,  Mima Mujacic ,  Kenneth Mayo Prentice

IPC分类号： G01N33/53 ,  C12Q1/34 ,  G01N33/50

CPC分类号： G01N33/5308 ,  C12Q1/34 ,  C12Y305/01052 ,  G01N33/502 ,  G01N2400/10 ,  G01N2570/00

摘要： Provided herein are methods for assessing cell surface glycans, e.g., N-glycans, by assessing a sample of released surface glycans, and determining the presence, absence, or level of glycans present in the sample. Also provided are methods of assaying and/or evaluating a cell composition by assessing the cell surface glycan profile of the cell composition and comparing the profile to a reference sample. Methods for manufacturing and/or culturing a plurality of cell compositions having consistent surface glycan expression with low variability are also provided.