公开(公告)号：US09329180B2

公开(公告)日：2016-05-03

申请号：US14057999

申请日：2013-10-18

Applicant: KAOHSIUNG MEDICAL UNIVERSITY

Inventor： Tian-Lu Cheng ,  Steven R. Roffler ,  Kuo-Hsiang Chuang ,  Ssu-Jung Lu

IPC: G01N33/53 ,  G01N33/554 ,  G01N33/68 ,  G01N33/94 ,  C07K16/44

CPC classification number: G01N33/554 ,  C07K16/44 ,  C07K2317/56 ,  C07K2319/03 ,  C12N2503/02 ,  C12N2510/04 ,  G01N33/5308 ,  G01N33/6857 ,  G01N33/94 ,  G01N33/9493 ,  G01N2430/60 ,  G01N2440/28

Abstract: The present invention provides a method for preparing an isolated eukaryotic cell which presents an anti-polyethylene glycol (PEG) antibody on a cell membrane. The present invention also provides a method for a quantitative analysis of a polyethylene glycol (PEG) by said anti-PEG antibody expressing cell. The cell-based quantitative analysis of the present prevention could sensitively quantify free PEG and PEG-modified macromolecules (proteins, nanoparticles and liposomes) as sensitive as nano-gram level.

Abstract translation: 本发明提供了一种在细胞膜上提供抗聚乙二醇（PEG）抗体的分离的真核细胞的制备方法。 本发明还提供了通过所述抗-PEG抗体表达细胞定量分析聚乙二醇（PEG）的方法。 本发明预防的基于细胞的定量分析可以灵敏地定量游离的PEG和PEG修饰的大分子（蛋白质，纳米颗粒和脂质体）作为纳克级水平敏感。