公开(公告)号：US3674644A

公开(公告)日：1972-07-04

申请号：US3674644D

申请日：1969-12-12

申请人： KIKKOMAN SHOYU CO LTD

发明人： YOKOTSUKA TAMOTSU ,  HASHIMOTO HIKOTAKA ,  IWAASA TAKASHI

IPC分类号： C12N9/58 ,  C12D13/10

CPC分类号： C12N9/58 ,  Y10S435/933

摘要： New acidstable and thermostable protease which is active at pH 1.5-6.5; an optimum pH being 2.0-3.0; an optimum temperature being 60* C. at pH 2.5, 75* C. at 3.5 and 75*-80* C. at 4.5. The protease has a strong liquefaction power and makes free a very small amount of amino acids, e.g., amino state N: total N is less than 18, when a substrate of defatted soybean is decomposed. The protease is useful for the food industry, fermentation industry and animal feed industry. The protease is produced by culturing Penicillium duponti ATCC 20186 in a solid or liquid medium at a temperature of, preferably, 40*-50* C. and at a pH of 4.0-7.0.

摘要翻译： 新的酸稳定和热稳定的蛋白酶，其活性在pH 1.5-6.5; 最适pH为2.0-3.0; 最适温度为60℃，pH 2.5，75℃，3.5和75°-80℃，4.5。