公开(公告)号：US12228496B2

公开(公告)日：2025-02-18

申请号：US17083299

申请日：2020-10-29

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Myung-Suk Chun ,  Sun Mi Lee ,  Jae Hun Kim ,  Chansung Park

IPC: G01N15/14 ,  B01L3/00 ,  G01N21/64

Abstract: Provided is an apparatus for cell particle sorting based on microfluidic-chip flow, by using a design in which Dean flow focusing occurring in a spiral channel and hydrodynamic filtration are coupled. The apparatus comprises a first substrate including a spiral channel having an inner surface and an outer surface based on a radius of curvature, a sample solution inlet, a medium inlet, and a spiral inner-outlet and a spiral outer-outlet both for discharging the particles, and a second substrate including a main channel in which the sample solution discharged from the first substrate and passing through an inter-substrate way flows and a cut-off width WC is set, a side channel allowing a medium introduced into the medium inlet to flow to focus the sample solution on a sidewall of the main channel, a plurality of branch channels connected to the sidewall of main channel and configured to receive the particles from the main channel, a main channel outlet, and at least one branch channel outlet.