公开(公告)号：US20130251784A1

公开(公告)日：2013-09-26

申请号：US13774247

申请日：2013-02-22

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Kwang Meyung KIM ,  Ick Chan KWON ,  Kuiwon CHOI ,  Heebeom KOO ,  Sang-min LEE ,  Inchan YOUN

IPC: A61K47/26 ,  A61K47/34

CPC classification number: A61K47/26 ,  A61K47/34 ,  A61K47/549 ,  A61K47/555 ,  A61K47/6911 ,  A61K49/0032 ,  A61K49/0052

Abstract: The present disclosure relates to a method for in vivo targeting of a nanoparticle via bioorthogonal copper-free click chemistry, more particularly to a method for in vivo targeting of a nanoparticle, including: injecting a precursor capable of being metabolically engineered in vivo when injected into a living system and having a first bioorthogonal functional group into the living system; and injecting a nanoparticle having a second bioorthogonal functional group which can perform a bioorthogonal copper-free click reaction with the first bioorthogonal functional group attached thereto into the living system.In accordance with the present disclosure, accumulation of nanoparticles at a target site in a living system can be increased remarkably and the biodistribution of the nanoparticles can be controlled since the nanoparticles bound to a cell surface are taken up into the cell with time.

Abstract translation: 本公开内容涉及一种通过生物正交无铜点击化学体内靶向纳米颗粒的方法，更具体地涉及纳米颗粒的体内靶向方法，包括：注射能够在注射入体内时被代谢工程化的前体 生活系统，并具有第一生物正交功能组进入生活系统; 并且将具有第二生物正交官能团的纳米颗粒注射到与生物体系附着的第一生物正交官能团进行生物正交无铜点击反应。 根据本公开，可以显着增加生物体系中靶位点上的纳米颗粒的积累，并且可以控制纳米颗粒的生物分布，因为结合到细胞表面的纳米颗粒随时间被吸收到细胞中。