公开(公告)号：US20200239944A1

公开(公告)日：2020-07-30

申请号：US16628346

申请日：2018-07-03

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Kyung KIM ,  Junsun KIM

IPC: C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6853

Abstract: In the present invention, when amplifying a nucleic acid by incorporating at least one primer among a forward primer and reverse primer and/or a probe in an upper critical solution temperature (UCST) particle, or when amplifying a nucleic acid by incorporating at least one primer among the forward primer and reverse primer and/or a probe in a UCST particle and fixing to a hydrogel fine particle, same the primer or probe comprised in the UCST particle can be discharged within a certain temperature range. Accordingly, the formation of primer dimers can be prevented while also achieving excellent PCR amplification efficiency.